Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation
Introduction: Bioremediation has been shown to be an effective strategy for removing toxic pollutants from the environment, particularly organic chemicals such as petroleum hydrocarbons. This paper investigates the changes in toxicity of petroleum-contaminated soil as a result of microbial remediati...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2023.1141562/full |
| _version_ | 1827996513046888448 |
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| author | Xiaoyun Chai Mutian Wang Xiaowen Fu Wen Zhang Yujie Huang Kieran J. Germaine Jianing Wang |
| author_facet | Xiaoyun Chai Mutian Wang Xiaowen Fu Wen Zhang Yujie Huang Kieran J. Germaine Jianing Wang |
| author_sort | Xiaoyun Chai |
| collection | DOAJ |
| description | Introduction: Bioremediation has been shown to be an effective strategy for removing toxic pollutants from the environment, particularly organic chemicals such as petroleum hydrocarbons. This paper investigates the changes in toxicity of petroleum-contaminated soil as a result of microbial remediation processes.Methods: Changes in the ecotoxicity of the contaminated soil were examined using a plant, earthworm, enzyme activity and luminescent bacteria toxicity tests.Results: The results showed that bioremediation could effectively degrade petroleum hydrocarbon (C10–C40) pollutants. After 42 days of remediation, the petroleum hydrocarbon (C10–C40) content of Group A (bioaugmented polluted wetland soil) decreased from 1.66 g/kg to 1.00 g/kg, and the degradation rate was 40.6%. The petroleum hydrocarbon (C10–C40) content of Group B (bioaugmented polluted farmland soil decreased from 4.00 g/kg to 1.94 g/kg, and the degradation rate was 51.6%. During the microbial remediation progress, the ecological toxicity of petroleum-contaminated soil first increased and then decreased. The photosynthetic pigment content index in the higher plant toxicity test, the earthworm survival index and the soil catalase activity all showed good agreement with the relative luminescence index of extracted DCM/DMSO in the luminescent bacterial toxicity test. The soil toxicity decreased significantly after remediation. Specifically, the photosynthetic pigment content of wheat were inhibited in the soil during the whole process (remediation for 42 days), and decreased to the minimum on remediation day 21. The 7-day and 14-day survival rate of earthworms in Group A and Group B gradually decreased in the soil remediation process, and then gradually increased, survival rate at the end of remediation was higher than at the beginning. Soil catalase activity was significantly negatively correlated with petroleum hydrocarbon (C10–C40) content (−0.988, −0.989). The ecological toxicity of contaminated soil reached to the maximum on the 21st day of remediation, relative luminosity of luminescent bacteria in dichloromethane/dimethyl sulfoxide extracts from Group A and Group B were 26.3% and 16.3%, respectively.Conclusion: Bioremediation could effectively degrade petroleum hydrocarbon (C10–C40) pollutants. Wheat photosynthetic pigment content, earthworm survival rate, soil catalase activity and relative luminescence of luminescent bacteria can better indicate the ecological toxicity of petroleum-contaminated soil in bioremediation process. |
| first_indexed | 2024-04-10T05:13:03Z |
| format | Article |
| id | doaj.art-c44507648fcd4d478589ccd5de224590 |
| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-04-10T05:13:03Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Environmental Science |
| spelling | doaj.art-c44507648fcd4d478589ccd5de2245902023-03-09T06:40:49ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2023-03-011110.3389/fenvs.2023.11415621141562Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediationXiaoyun Chai0Mutian Wang1Xiaowen Fu2Wen Zhang3Yujie Huang4Kieran J. Germaine5Jianing Wang6Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaEnviroCORE, Dargan Research Centre, South East Technological University, Carlow Campus, Carlow, IrelandShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaEnviroCORE, Dargan Research Centre, South East Technological University, Carlow Campus, Carlow, IrelandShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaIntroduction: Bioremediation has been shown to be an effective strategy for removing toxic pollutants from the environment, particularly organic chemicals such as petroleum hydrocarbons. This paper investigates the changes in toxicity of petroleum-contaminated soil as a result of microbial remediation processes.Methods: Changes in the ecotoxicity of the contaminated soil were examined using a plant, earthworm, enzyme activity and luminescent bacteria toxicity tests.Results: The results showed that bioremediation could effectively degrade petroleum hydrocarbon (C10–C40) pollutants. After 42 days of remediation, the petroleum hydrocarbon (C10–C40) content of Group A (bioaugmented polluted wetland soil) decreased from 1.66 g/kg to 1.00 g/kg, and the degradation rate was 40.6%. The petroleum hydrocarbon (C10–C40) content of Group B (bioaugmented polluted farmland soil decreased from 4.00 g/kg to 1.94 g/kg, and the degradation rate was 51.6%. During the microbial remediation progress, the ecological toxicity of petroleum-contaminated soil first increased and then decreased. The photosynthetic pigment content index in the higher plant toxicity test, the earthworm survival index and the soil catalase activity all showed good agreement with the relative luminescence index of extracted DCM/DMSO in the luminescent bacterial toxicity test. The soil toxicity decreased significantly after remediation. Specifically, the photosynthetic pigment content of wheat were inhibited in the soil during the whole process (remediation for 42 days), and decreased to the minimum on remediation day 21. The 7-day and 14-day survival rate of earthworms in Group A and Group B gradually decreased in the soil remediation process, and then gradually increased, survival rate at the end of remediation was higher than at the beginning. Soil catalase activity was significantly negatively correlated with petroleum hydrocarbon (C10–C40) content (−0.988, −0.989). The ecological toxicity of contaminated soil reached to the maximum on the 21st day of remediation, relative luminosity of luminescent bacteria in dichloromethane/dimethyl sulfoxide extracts from Group A and Group B were 26.3% and 16.3%, respectively.Conclusion: Bioremediation could effectively degrade petroleum hydrocarbon (C10–C40) pollutants. Wheat photosynthetic pigment content, earthworm survival rate, soil catalase activity and relative luminescence of luminescent bacteria can better indicate the ecological toxicity of petroleum-contaminated soil in bioremediation process.https://www.frontiersin.org/articles/10.3389/fenvs.2023.1141562/fullsoilpetroleumbioremediationecotoxicitysoil enzyme activityluminescent bacteria |
| spellingShingle | Xiaoyun Chai Mutian Wang Xiaowen Fu Wen Zhang Yujie Huang Kieran J. Germaine Jianing Wang Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation Frontiers in Environmental Science soil petroleum bioremediation ecotoxicity soil enzyme activity luminescent bacteria |
| title | Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation |
| title_full | Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation |
| title_fullStr | Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation |
| title_full_unstemmed | Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation |
| title_short | Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation |
| title_sort | shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation |
| topic | soil petroleum bioremediation ecotoxicity soil enzyme activity luminescent bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2023.1141562/full |
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