Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles
Summary: Drosophila flight muscles are highly enriched with mitochondria and have emerged as a powerful genetic system for studying how oxidative phosphorylation (OXPHOS) complexes are assembled. Here, we describe a series of protocols for analyzing the integrity of OXPHOS complexes in Drosophila vi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721007279 |
| _version_ | 1819099576795136000 |
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| author | Anjaneyulu Murari Edward Owusu-Ansah |
| author_facet | Anjaneyulu Murari Edward Owusu-Ansah |
| author_sort | Anjaneyulu Murari |
| collection | DOAJ |
| description | Summary: Drosophila flight muscles are highly enriched with mitochondria and have emerged as a powerful genetic system for studying how oxidative phosphorylation (OXPHOS) complexes are assembled. Here, we describe a series of protocols for analyzing the integrity of OXPHOS complexes in Drosophila via blue native polyacrylamide gel electrophoresis (BN PAGE). We have also included protocols for the additional steps that are typically performed after OXPHOS complexes are separated by BN PAGE, such as Coomassie staining, silver staining, and in-gel OXPHOS activities.For complete details on the use and execution of this protocol, please refer to Murari et al. (2020). |
| first_indexed | 2024-12-22T00:49:05Z |
| format | Article |
| id | doaj.art-c4459a679fab457c935b3b9ece534873 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T00:49:05Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c4459a679fab457c935b3b9ece5348732022-12-21T18:44:29ZengElsevierSTAR Protocols2666-16672021-12-0124101021Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight musclesAnjaneyulu Murari0Edward Owusu-Ansah1Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA; Corresponding authorDepartment of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA; Corresponding authorSummary: Drosophila flight muscles are highly enriched with mitochondria and have emerged as a powerful genetic system for studying how oxidative phosphorylation (OXPHOS) complexes are assembled. Here, we describe a series of protocols for analyzing the integrity of OXPHOS complexes in Drosophila via blue native polyacrylamide gel electrophoresis (BN PAGE). We have also included protocols for the additional steps that are typically performed after OXPHOS complexes are separated by BN PAGE, such as Coomassie staining, silver staining, and in-gel OXPHOS activities.For complete details on the use and execution of this protocol, please refer to Murari et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721007279Cell BiologyGeneticsMetabolismModel OrganismsProtein Biochemistry |
| spellingShingle | Anjaneyulu Murari Edward Owusu-Ansah Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles STAR Protocols Cell Biology Genetics Metabolism Model Organisms Protein Biochemistry |
| title | Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles |
| title_full | Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles |
| title_fullStr | Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles |
| title_full_unstemmed | Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles |
| title_short | Analyzing the integrity of oxidative phosphorylation complexes in Drosophila flight muscles |
| title_sort | analyzing the integrity of oxidative phosphorylation complexes in drosophila flight muscles |
| topic | Cell Biology Genetics Metabolism Model Organisms Protein Biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721007279 |
| work_keys_str_mv | AT anjaneyulumurari analyzingtheintegrityofoxidativephosphorylationcomplexesindrosophilaflightmuscles AT edwardowusuansah analyzingtheintegrityofoxidativephosphorylationcomplexesindrosophilaflightmuscles |