Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps
High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexi...
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MDPI AG
2020-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/4/442 |
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| author | Moeto Nagai Keita Kato Satoshi Soga Tuhin Subhra Santra Takayuki Shibata |
| author_facet | Moeto Nagai Keita Kato Satoshi Soga Tuhin Subhra Santra Takayuki Shibata |
| author_sort | Moeto Nagai |
| collection | DOAJ |
| description | High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. The flow was bidirectionally controlled by changing the DC bias. A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The application of DC-biased AC voltage (100 kHz, 10 V<sub>pp</sub>, and V<sub>DC</sub>: −4 V) provided a sufficient electroosmotic flow outside the nozzle array. This integration method of nozzle and pumps is anticipated to be a standard integration method. The operating conditions of DC-biased AC electrokinetic pumps in a biological buffer was clarified and found useful for cell manipulation. |
| first_indexed | 2024-03-10T20:17:42Z |
| format | Article |
| id | doaj.art-c445a745e8cf40e899764d7771b55ed0 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T20:17:42Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| series | Micromachines |
| spelling | doaj.art-c445a745e8cf40e899764d7771b55ed02023-11-19T22:25:49ZengMDPI AGMicromachines2072-666X2020-04-0111444210.3390/mi11040442Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic PumpsMoeto Nagai0Keita Kato1Satoshi Soga2Tuhin Subhra Santra3Takayuki Shibata4Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, JapanDepartment of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, JapanDepartment of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, JapanDepartment of Engineering Design, Indian Institute of Technology Madras, Tamil Nadu 600036, IndiaDepartment of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, JapanHigh throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. The flow was bidirectionally controlled by changing the DC bias. A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The application of DC-biased AC voltage (100 kHz, 10 V<sub>pp</sub>, and V<sub>DC</sub>: −4 V) provided a sufficient electroosmotic flow outside the nozzle array. This integration method of nozzle and pumps is anticipated to be a standard integration method. The operating conditions of DC-biased AC electrokinetic pumps in a biological buffer was clarified and found useful for cell manipulation.https://www.mdpi.com/2072-666X/11/4/442micronozzle-arrayparallel cell manipulationbidirectional electrokinetic pumpDC biased AC electrokinetic flow |
| spellingShingle | Moeto Nagai Keita Kato Satoshi Soga Tuhin Subhra Santra Takayuki Shibata Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps Micromachines micronozzle-array parallel cell manipulation bidirectional electrokinetic pump DC biased AC electrokinetic flow |
| title | Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps |
| title_full | Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps |
| title_fullStr | Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps |
| title_full_unstemmed | Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps |
| title_short | Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps |
| title_sort | scalable parallel manipulation of single cells using micronozzle array integrated with bidirectional electrokinetic pumps |
| topic | micronozzle-array parallel cell manipulation bidirectional electrokinetic pump DC biased AC electrokinetic flow |
| url | https://www.mdpi.com/2072-666X/11/4/442 |
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