Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification
The rapid purification of biomaterials such as DNA, RNA, and antibodies has attracted extensive attention, and research interest has increased further with the COVID-19 pandemic. In particular, core–shell-structured superparamagnetic nanoparticles have been continuously studied for their application...
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MDPI AG
2021-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/12/3422 |
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| author | Jin Soon Han Gye Seok An |
| author_facet | Jin Soon Han Gye Seok An |
| author_sort | Jin Soon Han |
| collection | DOAJ |
| description | The rapid purification of biomaterials such as DNA, RNA, and antibodies has attracted extensive attention, and research interest has increased further with the COVID-19 pandemic. In particular, core–shell-structured superparamagnetic nanoparticles have been continuously studied for their application as biopurification materials. It has been reported that Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> nanoparticles are one of the most promising candidates for separating nucleic acids via a simple and rapid process. This study proposed a fabrication method for dual-layered Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> nanoparticles, in which the density of the SiO<sub>2</sub> shell was controlled using an intermediate surfactant during the SiO<sub>2</sub> coating. After the fabrication of dual-layered Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> nanoparticles, structural, morphological, and magnetic analyses were conducted. The results showed that the Fe<sub>3</sub>O<sub>4</sub> nanoparticles were surrounded by a dense layer 15.6~27.9 nm thick and a porous layer 24.2~44.4 nm thick, and had superparamagnetic properties with high saturated magnetization at room temperature (86.9 emu/g). Then, the optimal conditions for the biopurification material were suggested based on analysis of the selective separation of plasmid DNA. |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:26:42Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-c445b26918334f5399b5b29d8cab97212023-11-23T09:52:23ZengMDPI AGNanomaterials2079-49912021-12-011112342210.3390/nano11123422Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA PurificationJin Soon Han0Gye Seok An1Division of Materials Science and Engineering, Hanyang University, Seoul 04763, KoreaDepartment of Advanced Material Engineering, Kyonggi University, Suwon 16227, KoreaThe rapid purification of biomaterials such as DNA, RNA, and antibodies has attracted extensive attention, and research interest has increased further with the COVID-19 pandemic. In particular, core–shell-structured superparamagnetic nanoparticles have been continuously studied for their application as biopurification materials. It has been reported that Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> nanoparticles are one of the most promising candidates for separating nucleic acids via a simple and rapid process. This study proposed a fabrication method for dual-layered Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> nanoparticles, in which the density of the SiO<sub>2</sub> shell was controlled using an intermediate surfactant during the SiO<sub>2</sub> coating. After the fabrication of dual-layered Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> nanoparticles, structural, morphological, and magnetic analyses were conducted. The results showed that the Fe<sub>3</sub>O<sub>4</sub> nanoparticles were surrounded by a dense layer 15.6~27.9 nm thick and a porous layer 24.2~44.4 nm thick, and had superparamagnetic properties with high saturated magnetization at room temperature (86.9 emu/g). Then, the optimal conditions for the biopurification material were suggested based on analysis of the selective separation of plasmid DNA.https://www.mdpi.com/2079-4991/11/12/3422Fe<sub>3</sub>O<sub>4</sub>nanocompositesmagnetic propertiesSiO<sub>2</sub>plasmid DNA purification |
| spellingShingle | Jin Soon Han Gye Seok An Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification Nanomaterials Fe<sub>3</sub>O<sub>4</sub> nanocomposites magnetic properties SiO<sub>2</sub> plasmid DNA purification |
| title | Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification |
| title_full | Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification |
| title_fullStr | Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification |
| title_full_unstemmed | Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification |
| title_short | Preparation of Dual-Layered Core–Shell Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Nanoparticles and Their Properties of Plasmid DNA Purification |
| title_sort | preparation of dual layered core shell fe sub 3 sub o sub 4 sub sio sub 2 sub nanoparticles and their properties of plasmid dna purification |
| topic | Fe<sub>3</sub>O<sub>4</sub> nanocomposites magnetic properties SiO<sub>2</sub> plasmid DNA purification |
| url | https://www.mdpi.com/2079-4991/11/12/3422 |
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