Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it>
<p>Abstract</p> <p>Background</p> <p><it>Beauveria bassiana </it>is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. Although reported to be non-toxic to vertebrates, the potential allerge...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-01-01
|
| Series: | Clinical and Molecular Allergy |
| Online Access: | http://www.clinicalmolecularallergy.com/content/3/1/1 |
| _version_ | 1818834740146339840 |
|---|---|
| author | Keyhani Nemat O Huang Shih-Wen Westwood Greg S |
| author_facet | Keyhani Nemat O Huang Shih-Wen Westwood Greg S |
| author_sort | Keyhani Nemat O |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Beauveria bassiana </it>is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. Although reported to be non-toxic to vertebrates, the potential allergenicity of <it>Beauveria </it>species has not been widely studied.</p> <p>Methods</p> <p>IgE-reactivity studies were performed using sera from patients displaying mould hypersensitivity by immunoblot and immunoblot inhibition. Skin reactivity to <it>B. bassiana </it>extracts was measured using intradermal skin testing.</p> <p>Results</p> <p>Immunoblots of fungal extracts with pooled as well as individual sera showed a distribution of IgE reactive proteins present in <it>B. bassiana </it>crude extracts. Proteinase K digestion of extracts resulted in loss of IgE reactive epitopes, whereas EndoH and PNGaseF (glycosidase) treatments resulted in minor changes in IgE reactive banding patterns as determined by Western blots. Immunoblot inhibitions experiments showed complete loss of IgE-binding using self protein, and partial inhibition using extracts from common allergenic fungi including; <it>Alternaria alternata</it>, <it>Aspergillus fumigatus</it>, <it>Cladosporium herbarum</it>, <it>Candida albicans</it>, <it>Epicoccum purpurascens</it>, and <it>Penicillium notatum</it>. Several proteins including a strongly reactive band with an approximate molecular mass of 35 kDa was uninhibited by any of the tested extracts, and may represent <it>B. bassiana </it>specific allergens. Intradermal skin testing confirmed the <it>in vitro </it>results, demonstrating allergenic reactions in a number of individuals, including those who have had occupational exposure to <it>B. bassiana</it>.</p> <p>Conclusions</p> <p><it>Beauveria bassiana </it>possesses numerous IgE reactive proteins, some of which are cross-reactive among allergens from other fungi. A strongly reactive potential <it>B. bassiana </it>specific allergen (35 kDa) was identified. Intradermal skin testing confirmed the allergenic potential of <it>B. bassiana</it>.</p> |
| first_indexed | 2024-12-19T02:39:37Z |
| format | Article |
| id | doaj.art-c445ddc72c1542299a8bc745e3a80678 |
| institution | Directory Open Access Journal |
| issn | 1476-7961 |
| language | English |
| last_indexed | 2024-12-19T02:39:37Z |
| publishDate | 2005-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical and Molecular Allergy |
| spelling | doaj.art-c445ddc72c1542299a8bc745e3a806782022-12-21T20:39:10ZengBMCClinical and Molecular Allergy1476-79612005-01-0131110.1186/1476-7961-3-1Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it>Keyhani Nemat OHuang Shih-WenWestwood Greg S<p>Abstract</p> <p>Background</p> <p><it>Beauveria bassiana </it>is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. Although reported to be non-toxic to vertebrates, the potential allergenicity of <it>Beauveria </it>species has not been widely studied.</p> <p>Methods</p> <p>IgE-reactivity studies were performed using sera from patients displaying mould hypersensitivity by immunoblot and immunoblot inhibition. Skin reactivity to <it>B. bassiana </it>extracts was measured using intradermal skin testing.</p> <p>Results</p> <p>Immunoblots of fungal extracts with pooled as well as individual sera showed a distribution of IgE reactive proteins present in <it>B. bassiana </it>crude extracts. Proteinase K digestion of extracts resulted in loss of IgE reactive epitopes, whereas EndoH and PNGaseF (glycosidase) treatments resulted in minor changes in IgE reactive banding patterns as determined by Western blots. Immunoblot inhibitions experiments showed complete loss of IgE-binding using self protein, and partial inhibition using extracts from common allergenic fungi including; <it>Alternaria alternata</it>, <it>Aspergillus fumigatus</it>, <it>Cladosporium herbarum</it>, <it>Candida albicans</it>, <it>Epicoccum purpurascens</it>, and <it>Penicillium notatum</it>. Several proteins including a strongly reactive band with an approximate molecular mass of 35 kDa was uninhibited by any of the tested extracts, and may represent <it>B. bassiana </it>specific allergens. Intradermal skin testing confirmed the <it>in vitro </it>results, demonstrating allergenic reactions in a number of individuals, including those who have had occupational exposure to <it>B. bassiana</it>.</p> <p>Conclusions</p> <p><it>Beauveria bassiana </it>possesses numerous IgE reactive proteins, some of which are cross-reactive among allergens from other fungi. A strongly reactive potential <it>B. bassiana </it>specific allergen (35 kDa) was identified. Intradermal skin testing confirmed the allergenic potential of <it>B. bassiana</it>.</p>http://www.clinicalmolecularallergy.com/content/3/1/1 |
| spellingShingle | Keyhani Nemat O Huang Shih-Wen Westwood Greg S Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it> Clinical and Molecular Allergy |
| title | Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it> |
| title_full | Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it> |
| title_fullStr | Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it> |
| title_full_unstemmed | Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it> |
| title_short | Allergens of the entomopathogenic fungus <it>Beauveria bassiana</it> |
| title_sort | allergens of the entomopathogenic fungus it beauveria bassiana it |
| url | http://www.clinicalmolecularallergy.com/content/3/1/1 |
| work_keys_str_mv | AT keyhaninemato allergensoftheentomopathogenicfungusitbeauveriabassianait AT huangshihwen allergensoftheentomopathogenicfungusitbeauveriabassianait AT westwoodgregs allergensoftheentomopathogenicfungusitbeauveriabassianait |