Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis
Abstract A disruption in the expression of gga-miR-365-3p was confirmed in the Mycoplasma gallisepticum (MG)-infected Chicken primary alveolar type II epithelial (CP-II) cells based on previous sequencing results, but the role it plays in the infection was unclear. In the present study, we demonstra...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-12-01
|
| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-022-01117-x |
| _version_ | 1811179209507209216 |
|---|---|
| author | Yingjie Wang Yun Han Lulu Wang Mengyun Zou Yingfei Sun Huanling Sun Qiao Guo Xiuli Peng |
| author_facet | Yingjie Wang Yun Han Lulu Wang Mengyun Zou Yingfei Sun Huanling Sun Qiao Guo Xiuli Peng |
| author_sort | Yingjie Wang |
| collection | DOAJ |
| description | Abstract A disruption in the expression of gga-miR-365-3p was confirmed in the Mycoplasma gallisepticum (MG)-infected Chicken primary alveolar type II epithelial (CP-II) cells based on previous sequencing results, but the role it plays in the infection was unclear. In the present study, we demonstrate that MG evaded cellular host immunity via a gga-miR-365-3p/SOCS5-JAK/STATs negative feedback loop. Specifically, we found that at the initial stage of MG infection in cells, gga-miR-365-3p was rapidly increased and activated the JAK/STAT signaling pathway by inhibiting SOCS5, which induced the secretion of inflammatory factors and triggered immune response against MG infection. Over time, though, the infection progressed, MG gradually destroyed the immune defences of CP-II cells. In late stages of infection, MG escaped host immunity by reducing intracellular gga-miR-365-3p and inhibiting the JAK/STAT pathway to suppress the secretion of inflammatory factors and promote MG adhesion or invasion. These results revealed the game between MG and host cell interactions, providing a new perspective to gain insight into the pathogenic mechanisms of MG or other pathogens. Meanwhile, they also contributed to novel thoughts on the prevention and control of MG and other pathogenic infections, shedding light on the immune modulating response triggered by pathogen invasion and their molecular targeting. Graphic Abstract |
| first_indexed | 2024-04-11T06:31:01Z |
| format | Article |
| id | doaj.art-c44611c89fb04cd48ff603f7a20d929a |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-04-11T06:31:01Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-c44611c89fb04cd48ff603f7a20d929a2022-12-22T04:40:05ZengBMCVeterinary Research1297-97162022-12-0153111410.1186/s13567-022-01117-xMycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axisYingjie Wang0Yun Han1Lulu Wang2Mengyun Zou3Yingfei Sun4Huanling Sun5Qiao Guo6Xiuli Peng7Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityAbstract A disruption in the expression of gga-miR-365-3p was confirmed in the Mycoplasma gallisepticum (MG)-infected Chicken primary alveolar type II epithelial (CP-II) cells based on previous sequencing results, but the role it plays in the infection was unclear. In the present study, we demonstrate that MG evaded cellular host immunity via a gga-miR-365-3p/SOCS5-JAK/STATs negative feedback loop. Specifically, we found that at the initial stage of MG infection in cells, gga-miR-365-3p was rapidly increased and activated the JAK/STAT signaling pathway by inhibiting SOCS5, which induced the secretion of inflammatory factors and triggered immune response against MG infection. Over time, though, the infection progressed, MG gradually destroyed the immune defences of CP-II cells. In late stages of infection, MG escaped host immunity by reducing intracellular gga-miR-365-3p and inhibiting the JAK/STAT pathway to suppress the secretion of inflammatory factors and promote MG adhesion or invasion. These results revealed the game between MG and host cell interactions, providing a new perspective to gain insight into the pathogenic mechanisms of MG or other pathogens. Meanwhile, they also contributed to novel thoughts on the prevention and control of MG and other pathogenic infections, shedding light on the immune modulating response triggered by pathogen invasion and their molecular targeting. Graphic Abstracthttps://doi.org/10.1186/s13567-022-01117-xMycoplasma gallisepticumGga-miR-365-3pSOCS5immune escapeJAK/STAT |
| spellingShingle | Yingjie Wang Yun Han Lulu Wang Mengyun Zou Yingfei Sun Huanling Sun Qiao Guo Xiuli Peng Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis Veterinary Research Mycoplasma gallisepticum Gga-miR-365-3p SOCS5 immune escape JAK/STAT |
| title | Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis |
| title_full | Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis |
| title_fullStr | Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis |
| title_full_unstemmed | Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis |
| title_short | Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis |
| title_sort | mycoplasma gallisepticum escapes the host immune response via gga mir 365 3p socs5 stats axis |
| topic | Mycoplasma gallisepticum Gga-miR-365-3p SOCS5 immune escape JAK/STAT |
| url | https://doi.org/10.1186/s13567-022-01117-x |
| work_keys_str_mv | AT yingjiewang mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT yunhan mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT luluwang mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT mengyunzou mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT yingfeisun mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT huanlingsun mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT qiaoguo mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis AT xiulipeng mycoplasmagallisepticumescapesthehostimmuneresponseviaggamir3653psocs5statsaxis |