The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.

The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection....

Full description

Bibliographic Details
Main Authors: Lance R Fredericks, Mark D Lee, Cooper R Roslund, Angela M Crabtree, Peter B Allen, Paul A Rowley
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0230767
_version_ 1819112118796943360
author Lance R Fredericks
Mark D Lee
Cooper R Roslund
Angela M Crabtree
Peter B Allen
Paul A Rowley
author_facet Lance R Fredericks
Mark D Lee
Cooper R Roslund
Angela M Crabtree
Peter B Allen
Paul A Rowley
author_sort Lance R Fredericks
collection DOAJ
description The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrain G. mellonella larvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection.
first_indexed 2024-12-22T04:08:26Z
format Article
id doaj.art-c446d3e95ee649a7ad046bec36a484b4
institution Directory Open Access Journal
issn 1932-6203
language English
last_indexed 2024-12-22T04:08:26Z
publishDate 2020-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj.art-c446d3e95ee649a7ad046bec36a484b42022-12-21T18:39:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01157e023076710.1371/journal.pone.0230767The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.Lance R FredericksMark D LeeCooper R RoslundAngela M CrabtreePeter B AllenPaul A RowleyThe injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrain G. mellonella larvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection.https://doi.org/10.1371/journal.pone.0230767
spellingShingle Lance R Fredericks
Mark D Lee
Cooper R Roslund
Angela M Crabtree
Peter B Allen
Paul A Rowley
The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
PLoS ONE
title The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
title_full The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
title_fullStr The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
title_full_unstemmed The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
title_short The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
title_sort design and implementation of restraint devices for the injection of pathogenic microorganisms into galleria mellonella
url https://doi.org/10.1371/journal.pone.0230767
work_keys_str_mv AT lancerfredericks thedesignandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT markdlee thedesignandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT cooperrroslund thedesignandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT angelamcrabtree thedesignandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT peterballen thedesignandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT paularowley thedesignandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT lancerfredericks designandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT markdlee designandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT cooperrroslund designandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT angelamcrabtree designandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT peterballen designandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella
AT paularowley designandimplementationofrestraintdevicesfortheinjectionofpathogenicmicroorganismsintogalleriamellonella