The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.
The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection....
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0230767 |
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author | Lance R Fredericks Mark D Lee Cooper R Roslund Angela M Crabtree Peter B Allen Paul A Rowley |
author_facet | Lance R Fredericks Mark D Lee Cooper R Roslund Angela M Crabtree Peter B Allen Paul A Rowley |
author_sort | Lance R Fredericks |
collection | DOAJ |
description | The injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrain G. mellonella larvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection. |
first_indexed | 2024-12-22T04:08:26Z |
format | Article |
id | doaj.art-c446d3e95ee649a7ad046bec36a484b4 |
institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-12-22T04:08:26Z |
publishDate | 2020-01-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS ONE |
spelling | doaj.art-c446d3e95ee649a7ad046bec36a484b42022-12-21T18:39:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01157e023076710.1371/journal.pone.0230767The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella.Lance R FredericksMark D LeeCooper R RoslundAngela M CrabtreePeter B AllenPaul A RowleyThe injection of laboratory animals with pathogenic microorganisms poses a significant safety risk because of the potential for injury by accidental needlestick. This is especially true for researchers using invertebrate models of disease due to the required precision and accuracy of the injection. The restraint of the greater wax moth larvae (Galleria mellonella) is often achieved by grasping a larva firmly between finger and thumb. Needle resistant gloves or forceps can be used to reduce the risk of a needlestick but can result in animal injury, a loss of throughput, and inconsistencies in experimental data. Restraint devices are commonly used for the manipulation of small mammals, and in this manuscript, we describe the construction of two devices that can be used to entrap and restrain G. mellonella larvae prior to injection with pathogenic microbes. These devices reduce the manual handling of larvae and provide an engineering control to protect against accidental needlestick injury while maintaining a high rate of injection.https://doi.org/10.1371/journal.pone.0230767 |
spellingShingle | Lance R Fredericks Mark D Lee Cooper R Roslund Angela M Crabtree Peter B Allen Paul A Rowley The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella. PLoS ONE |
title | The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella. |
title_full | The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella. |
title_fullStr | The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella. |
title_full_unstemmed | The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella. |
title_short | The design and implementation of restraint devices for the injection of pathogenic microorganisms into Galleria mellonella. |
title_sort | design and implementation of restraint devices for the injection of pathogenic microorganisms into galleria mellonella |
url | https://doi.org/10.1371/journal.pone.0230767 |
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