Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran
Background: Ventilator-associated pneumonia (VAP) is a common nosocomial infection among hospitalized patients who have undergone intubation and mechanical ventilation for more than 48 hours. Patients admitted to the intensive care unit (ICU) are at risk of developing life-threatening VAP due to spe...
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2018-10-01
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| Series: | Novelty in Biomedicine |
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| Online Access: | http://journals.sbmu.ac.ir/nbm/article/view/23198 |
| _version_ | 1819036336857808896 |
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| author | Seyed Hamid Borsi Maryam Haddadzadeh Shoushtari Hanieh Raji Faramarz Ghalavand |
| author_facet | Seyed Hamid Borsi Maryam Haddadzadeh Shoushtari Hanieh Raji Faramarz Ghalavand |
| author_sort | Seyed Hamid Borsi |
| collection | DOAJ |
| description | Background: Ventilator-associated pneumonia (VAP) is a common nosocomial infection among hospitalized patients who have undergone intubation and mechanical ventilation for more than 48 hours. Patients admitted to the intensive care unit (ICU) are at risk of developing life-threatening VAP due to specific conditions, especially with Gram-negative pathogens with advanced drug resistance. Hereby, the control of these agents and its monitoring is of particular importance. In this study, the pattern of antibiotic resistance of Gram-negative bacteria isolated from tracheal culture of patients with VAP investigated in ICU of Imam Khomeini Hospital of Ahwaz.
Materials and Methods: In this cross-sectional study, tracheal samples were collected during April 2016 to April 2017 from patients who were on mechanical ventilation in ICU of Imam Khomeini Hospital in Ahwaz, Khuzestan province, southwest of Iran. After isolation, bacterial strains were identified using biochemical tests. Then, antimicrobial resistance pattern of these isolates investigated using standard disc diffusion according to clinical and laboratory standards institute 2016 (CLSI 2016) guidelines.
Results: A total of 111 bacterial isolates were identified which were as following; Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Stenotrophomonas maltophilia, and Serratia marcescens, with prevalence of 54%, 19.8%, 14.4%, 6.4%, 4.5%, and 0.9%, respectively. Antibiotic susceptibility test of isolates showed that almost all isolates had high resistance to treatment antibiotics and were multi-drug resistance (MDR). The A. baumannii isolates were resistant to ciprofloxacin and piperacillin-tazobactam, but ampicillin-tazobactam had a good effect.
Conclusion: The results of this study showed that patients admitted to ICU due to their conditions of treatment are more likely to develop VAP by Gram-negative pathogens. The empirical treatment of VAP due to predominant bacterial causes and emerging drug resistance has become more challenging. It requires to use of multidrug regimens for routine clinical practice. It should be noted that in order to appropriate antimicrobial therapy, precise and correct diagnosis is very important. |
| first_indexed | 2024-12-21T08:03:55Z |
| format | Article |
| id | doaj.art-c44726c310a4449b974f27cd7a5f2f4b |
| institution | Directory Open Access Journal |
| issn | 2345-3346 2345-3907 |
| language | English |
| last_indexed | 2024-12-21T08:03:55Z |
| publishDate | 2018-10-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
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| series | Novelty in Biomedicine |
| spelling | doaj.art-c44726c310a4449b974f27cd7a5f2f4b2022-12-21T19:10:51ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-33462345-39072018-10-016416717310.22037/nbm.v6i4.2319811052Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, IranSeyed Hamid Borsi0Maryam Haddadzadeh Shoushtari1Hanieh Raji2Faramarz Ghalavand3Air pollution and Respiratory Disease Research Center, Joundishapour University of Medical Sciences, Ahvaz, IranAir pollution and Respiratory Disease Research Center, Joundishapour University of Medical Sciences, Ahvaz, IranAir pollution and Respiratory Disease Research Center, Joundishapour University of Medical Sciences, Ahvaz, IranAir pollution and Respiratory Disease Research Center, Joundishapour University of Medical Sciences, Ahvaz, IranBackground: Ventilator-associated pneumonia (VAP) is a common nosocomial infection among hospitalized patients who have undergone intubation and mechanical ventilation for more than 48 hours. Patients admitted to the intensive care unit (ICU) are at risk of developing life-threatening VAP due to specific conditions, especially with Gram-negative pathogens with advanced drug resistance. Hereby, the control of these agents and its monitoring is of particular importance. In this study, the pattern of antibiotic resistance of Gram-negative bacteria isolated from tracheal culture of patients with VAP investigated in ICU of Imam Khomeini Hospital of Ahwaz. Materials and Methods: In this cross-sectional study, tracheal samples were collected during April 2016 to April 2017 from patients who were on mechanical ventilation in ICU of Imam Khomeini Hospital in Ahwaz, Khuzestan province, southwest of Iran. After isolation, bacterial strains were identified using biochemical tests. Then, antimicrobial resistance pattern of these isolates investigated using standard disc diffusion according to clinical and laboratory standards institute 2016 (CLSI 2016) guidelines. Results: A total of 111 bacterial isolates were identified which were as following; Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Stenotrophomonas maltophilia, and Serratia marcescens, with prevalence of 54%, 19.8%, 14.4%, 6.4%, 4.5%, and 0.9%, respectively. Antibiotic susceptibility test of isolates showed that almost all isolates had high resistance to treatment antibiotics and were multi-drug resistance (MDR). The A. baumannii isolates were resistant to ciprofloxacin and piperacillin-tazobactam, but ampicillin-tazobactam had a good effect. Conclusion: The results of this study showed that patients admitted to ICU due to their conditions of treatment are more likely to develop VAP by Gram-negative pathogens. The empirical treatment of VAP due to predominant bacterial causes and emerging drug resistance has become more challenging. It requires to use of multidrug regimens for routine clinical practice. It should be noted that in order to appropriate antimicrobial therapy, precise and correct diagnosis is very important.http://journals.sbmu.ac.ir/nbm/article/view/23198Acinetobacter baumannii, Antibiotic resistance, Enterobacteria, ICU, ventilator-associated pneumonia |
| spellingShingle | Seyed Hamid Borsi Maryam Haddadzadeh Shoushtari Hanieh Raji Faramarz Ghalavand Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran Novelty in Biomedicine Acinetobacter baumannii, Antibiotic resistance, Enterobacteria, ICU, ventilator-associated pneumonia |
| title | Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran |
| title_full | Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran |
| title_fullStr | Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran |
| title_full_unstemmed | Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran |
| title_short | Prevalence of Gram-negative Bacteria Isolated from Patients with Ventilator-Associated Pneumonia in Intensive Care Units of Imam Khomeini Hospital, Ahwaz, Iran |
| title_sort | prevalence of gram negative bacteria isolated from patients with ventilator associated pneumonia in intensive care units of imam khomeini hospital ahwaz iran |
| topic | Acinetobacter baumannii, Antibiotic resistance, Enterobacteria, ICU, ventilator-associated pneumonia |
| url | http://journals.sbmu.ac.ir/nbm/article/view/23198 |
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