Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens)
In Germany, the wild ancestor (Apium graveolens L. ssp. graveolens) of celery and celeriac is threatened by genetic erosion. Seventy-eight potentially suitable genetic reserve sites representing differing ecogeographic units were assessed with regard to the conservation status of the populations. At...
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| Format: | Article |
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MDPI AG
2018-08-01
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| Series: | Agriculture |
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| Online Access: | http://www.mdpi.com/2077-0472/8/9/129 |
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| author | Lothar Frese Maria Bönisch Marion Nachtigall Uta Schirmak |
| author_facet | Lothar Frese Maria Bönisch Marion Nachtigall Uta Schirmak |
| author_sort | Lothar Frese |
| collection | DOAJ |
| description | In Germany, the wild ancestor (Apium graveolens L. ssp. graveolens) of celery and celeriac is threatened by genetic erosion. Seventy-eight potentially suitable genetic reserve sites representing differing ecogeographic units were assessed with regard to the conservation status of the populations. At 27 of the 78 sites, 30 individual plants were sampled and genetically analyzed with 16 polymorphic microsatellite makers. The Discriminant Analysis of Principal Components (DAPC) was applied to identify clusters of genetically similar individuals. In most cases (25 out of 27 occurrences) individuals clustered into groups according to their sampling site. Next to three clearly separated occurrences (AgG, AgUW, AgFEH) two large groups of inland and Baltic Sea coast occurrences, respectively, were recognized. Occurrences from the coastal part of the distribution area were interspersed into the group of inland occurrences and vice versa. The genetic distribution pattern is therefore complex. The complementary compositional genetic differentiation Δj was calculated to identify the Most Appropriate Wild Populations (MAWP) for the establishment of genetic reserves. Altogether 15 sites are recommended to form a genetic reserve network. This organisational structure appears suitable for promoting the in situ conservation of intraspecific genetic diversity and the species’ adaptability. As seed samples of each MAWP will be stored in a genebank, the network would likewise contribute to the long-term ex situ conservation of genetic resources for plant breeding. |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-12-16T17:01:27Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj.art-c447cd733a964e44a4de8ab176f36d232022-12-21T22:23:43ZengMDPI AGAgriculture2077-04722018-08-018912910.3390/agriculture8090129agriculture8090129Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens)Lothar Frese0Maria Bönisch1Marion Nachtigall2Uta Schirmak3Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Erwin-Baur-Str. 27, 06484 Quedlinburg, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Erwin-Baur-Str. 27, 06484 Quedlinburg, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Erwin-Baur-Str. 27, 06484 Quedlinburg, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Erwin-Baur-Str. 27, 06484 Quedlinburg, GermanyIn Germany, the wild ancestor (Apium graveolens L. ssp. graveolens) of celery and celeriac is threatened by genetic erosion. Seventy-eight potentially suitable genetic reserve sites representing differing ecogeographic units were assessed with regard to the conservation status of the populations. At 27 of the 78 sites, 30 individual plants were sampled and genetically analyzed with 16 polymorphic microsatellite makers. The Discriminant Analysis of Principal Components (DAPC) was applied to identify clusters of genetically similar individuals. In most cases (25 out of 27 occurrences) individuals clustered into groups according to their sampling site. Next to three clearly separated occurrences (AgG, AgUW, AgFEH) two large groups of inland and Baltic Sea coast occurrences, respectively, were recognized. Occurrences from the coastal part of the distribution area were interspersed into the group of inland occurrences and vice versa. The genetic distribution pattern is therefore complex. The complementary compositional genetic differentiation Δj was calculated to identify the Most Appropriate Wild Populations (MAWP) for the establishment of genetic reserves. Altogether 15 sites are recommended to form a genetic reserve network. This organisational structure appears suitable for promoting the in situ conservation of intraspecific genetic diversity and the species’ adaptability. As seed samples of each MAWP will be stored in a genebank, the network would likewise contribute to the long-term ex situ conservation of genetic resources for plant breeding.http://www.mdpi.com/2077-0472/8/9/129Apium graveolensgenetic resourcescrop wild relativein situ conservationmicrosatellite markergenetic distancegenetic differentiationgenetic reserve |
| spellingShingle | Lothar Frese Maria Bönisch Marion Nachtigall Uta Schirmak Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens) Agriculture Apium graveolens genetic resources crop wild relative in situ conservation microsatellite marker genetic distance genetic differentiation genetic reserve |
| title | Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens) |
| title_full | Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens) |
| title_fullStr | Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens) |
| title_full_unstemmed | Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens) |
| title_short | Patterns of Genetic Diversity and Implications for In Situ Conservation of Wild Celery (Apium graveolens L. ssp. graveolens) |
| title_sort | patterns of genetic diversity and implications for in situ conservation of wild celery apium graveolens l ssp graveolens |
| topic | Apium graveolens genetic resources crop wild relative in situ conservation microsatellite marker genetic distance genetic differentiation genetic reserve |
| url | http://www.mdpi.com/2077-0472/8/9/129 |
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