Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia
A new Eimeria species is described from a red-capped parrot (Purpureicephalus spurius). Sporulated oocysts (n = 31) were spherical to subspherical, with a rough bilayered oocyst wall 0.8 μm thick. Oocysts measured 24.0 × 22.8 (20.4–26.4 × 18.3–25.9) μm, oocyst length/width ratio, 1.10. Oocyst residu...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224416300037 |
| _version_ | 1819268375494262784 |
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| author | Rongchang Yang Belinda Brice Una Ryan |
| author_facet | Rongchang Yang Belinda Brice Una Ryan |
| author_sort | Rongchang Yang |
| collection | DOAJ |
| description | A new Eimeria species is described from a red-capped parrot (Purpureicephalus spurius). Sporulated oocysts (n = 31) were spherical to subspherical, with a rough bilayered oocyst wall 0.8 μm thick. Oocysts measured 24.0 × 22.8 (20.4–26.4 × 18.3–25.9) μm, oocyst length/width ratio, 1.10. Oocyst residuum, polar granule and micropyle were absent. Sporocysts are elongate-ovoid, 11.0 × 7.3 (12.7–9.2 × 7.9–6.6) μm, sporocyst length/width ratio, 1.51 (1.33–1.71). The thin convex Stieda body and indistinct substieda bodies were present and the sporocyst residuum was composed of numerous small granules less than 1.0 μm in diameter dispersed randomly. Each sporocyst contained 2 sausage-shaped sporozoites in head-to-tail arrangement. The sporozoite nuclei were located centrally surrounded by refractile bodies. Molecular analysis was conducted at two loci; the 18S ribosomal RNA gene and the cytochrome c oxidase subunit I gene. At the18S locus, the new isolate shared 99.0% genetic similarity with Eimeria dispersa and Eimeria innocua from the turkey. At the cytochrome c oxidase subunit I gene locus, this new isolate was most closely related to E. dispersa and E. innocua, presented 99.0% and 98.0% genetic similarity, respectively. This new isolate and E. dispersa grouped together in the same clade. Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria purpureicephali n. sp. after its host, the red-capped parrot (Purpureicephalus spurius). |
| first_indexed | 2024-12-23T21:32:04Z |
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| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-12-23T21:32:04Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
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| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-c44b1969321b4240bdb6aac0281fc9902022-12-21T17:30:26ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442016-04-0151343910.1016/j.ijppaw.2016.01.003Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western AustraliaRongchang Yang0Belinda Brice1Una Ryan2School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, AustraliaKanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, AustraliaSchool of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, AustraliaA new Eimeria species is described from a red-capped parrot (Purpureicephalus spurius). Sporulated oocysts (n = 31) were spherical to subspherical, with a rough bilayered oocyst wall 0.8 μm thick. Oocysts measured 24.0 × 22.8 (20.4–26.4 × 18.3–25.9) μm, oocyst length/width ratio, 1.10. Oocyst residuum, polar granule and micropyle were absent. Sporocysts are elongate-ovoid, 11.0 × 7.3 (12.7–9.2 × 7.9–6.6) μm, sporocyst length/width ratio, 1.51 (1.33–1.71). The thin convex Stieda body and indistinct substieda bodies were present and the sporocyst residuum was composed of numerous small granules less than 1.0 μm in diameter dispersed randomly. Each sporocyst contained 2 sausage-shaped sporozoites in head-to-tail arrangement. The sporozoite nuclei were located centrally surrounded by refractile bodies. Molecular analysis was conducted at two loci; the 18S ribosomal RNA gene and the cytochrome c oxidase subunit I gene. At the18S locus, the new isolate shared 99.0% genetic similarity with Eimeria dispersa and Eimeria innocua from the turkey. At the cytochrome c oxidase subunit I gene locus, this new isolate was most closely related to E. dispersa and E. innocua, presented 99.0% and 98.0% genetic similarity, respectively. This new isolate and E. dispersa grouped together in the same clade. Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria purpureicephali n. sp. after its host, the red-capped parrot (Purpureicephalus spurius).http://www.sciencedirect.com/science/article/pii/S221322441630003718S rRNAE. purpureicephali n. sp.MorphologyGenetic characterizationCytochrome c oxidase subunit I (COI) genePhylogeny |
| spellingShingle | Rongchang Yang Belinda Brice Una Ryan Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia International Journal for Parasitology: Parasites and Wildlife 18S rRNA E. purpureicephali n. sp. Morphology Genetic characterization Cytochrome c oxidase subunit I (COI) gene Phylogeny |
| title | Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia |
| title_full | Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia |
| title_fullStr | Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia |
| title_full_unstemmed | Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia |
| title_short | Morphological and molecular characterization of Eimeria purpureicephali n. sp. (Apicomplexa:Eimeriidae) in a red-capped parrot (Purpureicephalus spurius, Kuhl, 1820) in Western Australia |
| title_sort | morphological and molecular characterization of eimeria purpureicephali n sp apicomplexa eimeriidae in a red capped parrot purpureicephalus spurius kuhl 1820 in western australia |
| topic | 18S rRNA E. purpureicephali n. sp. Morphology Genetic characterization Cytochrome c oxidase subunit I (COI) gene Phylogeny |
| url | http://www.sciencedirect.com/science/article/pii/S2213224416300037 |
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