Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study
Summary: Aim: This study aimed to verify the efficacy of disinfection procedures to reduce Acinetobacter baumannii blaOXA-23 bacterial load in needleless connectors that had been experimentally contaminated. Methods: Two-way intermediate extender's hub and needle-free valve were contaminated w...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | Infection Prevention in Practice |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590088923000616 |
| _version_ | 1797300386807152640 |
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| author | C. Biazus-Dalcin T.C.M. Sincero C.P. Zamparette D.C. Tartari S. de Souza T.L. Silva A. Tomazoni P.K. Rocha |
| author_facet | C. Biazus-Dalcin T.C.M. Sincero C.P. Zamparette D.C. Tartari S. de Souza T.L. Silva A. Tomazoni P.K. Rocha |
| author_sort | C. Biazus-Dalcin |
| collection | DOAJ |
| description | Summary: Aim: This study aimed to verify the efficacy of disinfection procedures to reduce Acinetobacter baumannii blaOXA-23 bacterial load in needleless connectors that had been experimentally contaminated. Methods: Two-way intermediate extender's hub and needle-free valve were contaminated with Acinetobacter baumannii blaOXA-23. To disinfect them, the following procedures were carried out: sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% isopropyl alcohol single-use cap, with eight times friction for 10 s, followed by 5 s drying time. The statistical tests Kruskal–Wallis and post-hoc Conover were performed using MedCalc®. Results: A total of 82 experiments were conducted. All tested disinfection procedures were efficacious in reducing the A. baumannii blaOXA-23 load. The 70% IPA single-use cap was found to be the best method for disinfecting the two-way intermediate extender's hub (87.28%), while all the methods were efficacious for the disinfection of the needle-free valve (more than 90%). During the inoculation period, A. baumannii blaOXA-23 showed less adherence to the needle-free valve during the inoculation period, probably due to the device's design. Conclusion: The three tested disinfection procedures using sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% IPA single-use cap were found to be efficacious in reducing the bacterial load of A. baumanni blaOXA-23 in needleless connectors. Proper disinfection of needleless connectors is a crucial nursing practice to prevent bloodstream infections, as it significantly reduces the bacterial load present in the device. |
| first_indexed | 2024-03-07T23:05:27Z |
| format | Article |
| id | doaj.art-c44c9aefbe224c389d0c620a7636747d |
| institution | Directory Open Access Journal |
| issn | 2590-0889 |
| language | English |
| last_indexed | 2024-03-07T23:05:27Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Infection Prevention in Practice |
| spelling | doaj.art-c44c9aefbe224c389d0c620a7636747d2024-02-22T04:53:17ZengElsevierInfection Prevention in Practice2590-08892024-03-0161100328Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro studyC. Biazus-Dalcin0T.C.M. Sincero1C.P. Zamparette2D.C. Tartari3S. de Souza4T.L. Silva5A. Tomazoni6P.K. Rocha7School of Health Sciences, University of Dundee, Dundee, UK; Corresponding author. Address: School of Health Sciences, University of Dundee, 11 Airlie Place, Dundee, DD1 4HJ, UK. Tel.: +44 1382 385842.Department of Clinical Analyses, Health Sciences Centre, Universidade Federal de Santa Catarina (UFSC), Florianópolis, BrazilDepartment of Clinical Analyses, Health Sciences Centre, Universidade Federal de Santa Catarina (UFSC), Florianópolis, BrazilThe State of Santa Catarina, Florianópolis, BrazilNursing Department, Health Sciences Centre, Universidade Federal de Santa Catarina (UFSC), Florianópolis, BrazilNursing Department, Health Sciences Centre, Universidade Federal de Santa Catarina (UFSC), Florianópolis, BrazilHospital de Clínicas of the Universidade Federal do Paraná (UFPR), Curitiba, BrazilNursing Department, Health Sciences Centre, Universidade Federal de Santa Catarina (UFSC), Florianópolis, BrazilSummary: Aim: This study aimed to verify the efficacy of disinfection procedures to reduce Acinetobacter baumannii blaOXA-23 bacterial load in needleless connectors that had been experimentally contaminated. Methods: Two-way intermediate extender's hub and needle-free valve were contaminated with Acinetobacter baumannii blaOXA-23. To disinfect them, the following procedures were carried out: sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% isopropyl alcohol single-use cap, with eight times friction for 10 s, followed by 5 s drying time. The statistical tests Kruskal–Wallis and post-hoc Conover were performed using MedCalc®. Results: A total of 82 experiments were conducted. All tested disinfection procedures were efficacious in reducing the A. baumannii blaOXA-23 load. The 70% IPA single-use cap was found to be the best method for disinfecting the two-way intermediate extender's hub (87.28%), while all the methods were efficacious for the disinfection of the needle-free valve (more than 90%). During the inoculation period, A. baumannii blaOXA-23 showed less adherence to the needle-free valve during the inoculation period, probably due to the device's design. Conclusion: The three tested disinfection procedures using sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% IPA single-use cap were found to be efficacious in reducing the bacterial load of A. baumanni blaOXA-23 in needleless connectors. Proper disinfection of needleless connectors is a crucial nursing practice to prevent bloodstream infections, as it significantly reduces the bacterial load present in the device.http://www.sciencedirect.com/science/article/pii/S2590088923000616AcinetobacterBacterial loadCathetersCatheter-related infectionsDisinfection |
| spellingShingle | C. Biazus-Dalcin T.C.M. Sincero C.P. Zamparette D.C. Tartari S. de Souza T.L. Silva A. Tomazoni P.K. Rocha Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study Infection Prevention in Practice Acinetobacter Bacterial load Catheters Catheter-related infections Disinfection |
| title | Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study |
| title_full | Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study |
| title_fullStr | Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study |
| title_full_unstemmed | Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study |
| title_short | Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study |
| title_sort | efficacy of disinfection procedures to reduce acinetobacter baumanii blaoxa 23 contamination rate of needleless connectors an in vitro study |
| topic | Acinetobacter Bacterial load Catheters Catheter-related infections Disinfection |
| url | http://www.sciencedirect.com/science/article/pii/S2590088923000616 |
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