Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology
Taohong Siwu Decoction (TSD), a classical gynecological prescription that was firstly reported 600 years ago, has been widely used in the adjuvant treatment of breast cancer (BRCA) in China. However, the mechanism of action of TSD in treating BRCA has remained unclear. Here, high-throughput sequenci...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Computational and Structural Biotechnology Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037022002586 |
| _version_ | 1797978241825243136 |
|---|---|
| author | Yu Gui Yifei Dai Yumei Wang Shengrong Li Lei Xiang Yuqin Tang Xue Tan Tianli Pei Xilinqiqige Bao Dong Wang |
| author_facet | Yu Gui Yifei Dai Yumei Wang Shengrong Li Lei Xiang Yuqin Tang Xue Tan Tianli Pei Xilinqiqige Bao Dong Wang |
| author_sort | Yu Gui |
| collection | DOAJ |
| description | Taohong Siwu Decoction (TSD), a classical gynecological prescription that was firstly reported 600 years ago, has been widely used in the adjuvant treatment of breast cancer (BRCA) in China. However, the mechanism of action of TSD in treating BRCA has remained unclear. Here, high-throughput sequencing-based high-throughput screening (HTS2) technology was used to reveal the molecular mechanism of TSD, combination with bioinformatics and systems pharmacology in this study. Firstly, our results showed that TSD exerts an anticancer effect on BRCA cells by inhibiting cell proliferation, migration and inducing apoptosis as well as cell-cycle arrest. And our results from HTS2 suggested that herbs of TSD could significantly inhibit KRAS pathway and pathway in cancer, and activate apoptosis pathway, p53 pathway and hypoxia pathway, which may lead to the anticancer function of TSD. Further, we found that TSD clearly regulates MYC, BIRC5, EGF, and PIK3R1 genes, which play an important role in the development and progression of tumor and have significant correlation with overall survival in BRCA patients. By molecular docking, we discovered that Pentagalloylglucose, a compound derived from TSD, might directly bind to and inhibit the function of BRD4, which is a reported transcriptional activator of MYC gene, and thus repress the expression of MYC. Taken together, this study explores the mechanism of TSD in anti-BRCA by combining HTS2 technology, bioinformatics analysis and systems pharmacology. |
| first_indexed | 2024-04-11T05:19:52Z |
| format | Article |
| id | doaj.art-c44ea3de66fa438a84b2380bec92c2a2 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-04-11T05:19:52Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-c44ea3de66fa438a84b2380bec92c2a22022-12-24T04:53:08ZengElsevierComputational and Structural Biotechnology Journal2001-03702022-01-012034613472Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technologyYu Gui0Yifei Dai1Yumei Wang2Shengrong Li3Lei Xiang4Yuqin Tang5Xue Tan6Tianli Pei7Xilinqiqige Bao8Dong Wang9State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaDepartment of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaMedical Innovation Center for Nationalities, Inner Mongolia Medical University, Hohhot City 010110, China; Corresponding authors at: State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China (D. Wang).State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Corresponding authors at: State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China (D. Wang).Taohong Siwu Decoction (TSD), a classical gynecological prescription that was firstly reported 600 years ago, has been widely used in the adjuvant treatment of breast cancer (BRCA) in China. However, the mechanism of action of TSD in treating BRCA has remained unclear. Here, high-throughput sequencing-based high-throughput screening (HTS2) technology was used to reveal the molecular mechanism of TSD, combination with bioinformatics and systems pharmacology in this study. Firstly, our results showed that TSD exerts an anticancer effect on BRCA cells by inhibiting cell proliferation, migration and inducing apoptosis as well as cell-cycle arrest. And our results from HTS2 suggested that herbs of TSD could significantly inhibit KRAS pathway and pathway in cancer, and activate apoptosis pathway, p53 pathway and hypoxia pathway, which may lead to the anticancer function of TSD. Further, we found that TSD clearly regulates MYC, BIRC5, EGF, and PIK3R1 genes, which play an important role in the development and progression of tumor and have significant correlation with overall survival in BRCA patients. By molecular docking, we discovered that Pentagalloylglucose, a compound derived from TSD, might directly bind to and inhibit the function of BRD4, which is a reported transcriptional activator of MYC gene, and thus repress the expression of MYC. Taken together, this study explores the mechanism of TSD in anti-BRCA by combining HTS2 technology, bioinformatics analysis and systems pharmacology.http://www.sciencedirect.com/science/article/pii/S2001037022002586Taohong Siwu DecoctionBreast cancerHTS2 technologyBioinformaticsSystems pharmacology |
| spellingShingle | Yu Gui Yifei Dai Yumei Wang Shengrong Li Lei Xiang Yuqin Tang Xue Tan Tianli Pei Xilinqiqige Bao Dong Wang Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology Computational and Structural Biotechnology Journal Taohong Siwu Decoction Breast cancer HTS2 technology Bioinformatics Systems pharmacology |
| title | Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology |
| title_full | Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology |
| title_fullStr | Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology |
| title_full_unstemmed | Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology |
| title_short | Taohong Siwu Decoction exerts anticancer effects on breast cancer via regulating MYC, BIRC5, EGF and PIK3R1 revealed by HTS2 technology |
| title_sort | taohong siwu decoction exerts anticancer effects on breast cancer via regulating myc birc5 egf and pik3r1 revealed by hts2 technology |
| topic | Taohong Siwu Decoction Breast cancer HTS2 technology Bioinformatics Systems pharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S2001037022002586 |
| work_keys_str_mv | AT yugui taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT yifeidai taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT yumeiwang taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT shengrongli taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT leixiang taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT yuqintang taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT xuetan taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT tianlipei taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT xilinqiqigebao taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology AT dongwang taohongsiwudecoctionexertsanticancereffectsonbreastcancerviaregulatingmycbirc5egfandpik3r1revealedbyhts2technology |