Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae)
Abstract Introduction Plants are widely used in traditional medicine because they contain a high concentration of antimicrobial agents, serving as the foundation for medicines. The aim of this study was preliminary identification of phytochemicals and assesses the antimicrobial activity of extracts...
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.1170 |
| _version_ | 1797776617766912000 |
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| author | Betelihem Yirdaw Temesgen Kassa |
| author_facet | Betelihem Yirdaw Temesgen Kassa |
| author_sort | Betelihem Yirdaw |
| collection | DOAJ |
| description | Abstract Introduction Plants are widely used in traditional medicine because they contain a high concentration of antimicrobial agents, serving as the foundation for medicines. The aim of this study was preliminary identification of phytochemicals and assesses the antimicrobial activity of extracts of Ferula communis root bark. Methods Plant was collected, and standard qualitative procedures were conducted. The plant samples were extracted with 99.9% methanol and 80% ethanol. To identify phytochemicals found in plants, a preliminary phytochemical analysis was performed. Agar diffusion tests, minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were performed to evaluate antibacterial activity. Result The preliminary phytochemical analysis of the ethanol and methanol extract revealed positive results for flavonoids, coumarins and tannins. Terpenoids and anthraquinones were detected only in the methanol extract. The extract of Ferula communis showed an antibacterial effect on both gram‐negative and gram‐positive bacteria in a concentration‐dependent manner. The average zone of inhibition for gram‐positive bacteria was 11 mm, whereas for gram‐negative bacteria, it was 9 mm. The MIC and MBC values also varied with the type of bacteria. In all bacterial species tested, the mean MBC value was similar to the MIC. Conclusion Different phytochemicals were detected in extracts of the root bark of F. communis and extracts showed antibacterial effects in a concentration‐dependent manner. Therefore, further purification and evaluation of the extracts and antioxidant activity of the plant should be investigated. |
| first_indexed | 2024-03-12T22:52:24Z |
| format | Article |
| id | doaj.art-c45118ab570648bfb1891aa6a064fbf0 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T22:52:24Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-c45118ab570648bfb1891aa6a064fbf02023-07-20T08:09:46ZengWileyVeterinary Medicine and Science2053-10952023-07-01941901190710.1002/vms3.1170Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae)Betelihem Yirdaw0Temesgen Kassa1Assosa Agricultural Research Centre Ethiopian Institute of Agricultural Research AssosaEthiopiaHoleta Agricultural Research Centre Ethiopian Institute of Agricultural Research HoletaEthiopiaAbstract Introduction Plants are widely used in traditional medicine because they contain a high concentration of antimicrobial agents, serving as the foundation for medicines. The aim of this study was preliminary identification of phytochemicals and assesses the antimicrobial activity of extracts of Ferula communis root bark. Methods Plant was collected, and standard qualitative procedures were conducted. The plant samples were extracted with 99.9% methanol and 80% ethanol. To identify phytochemicals found in plants, a preliminary phytochemical analysis was performed. Agar diffusion tests, minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were performed to evaluate antibacterial activity. Result The preliminary phytochemical analysis of the ethanol and methanol extract revealed positive results for flavonoids, coumarins and tannins. Terpenoids and anthraquinones were detected only in the methanol extract. The extract of Ferula communis showed an antibacterial effect on both gram‐negative and gram‐positive bacteria in a concentration‐dependent manner. The average zone of inhibition for gram‐positive bacteria was 11 mm, whereas for gram‐negative bacteria, it was 9 mm. The MIC and MBC values also varied with the type of bacteria. In all bacterial species tested, the mean MBC value was similar to the MIC. Conclusion Different phytochemicals were detected in extracts of the root bark of F. communis and extracts showed antibacterial effects in a concentration‐dependent manner. Therefore, further purification and evaluation of the extracts and antioxidant activity of the plant should be investigated.https://doi.org/10.1002/vms3.1170antimicrobial effectsinhibition zonephytochemicalplant |
| spellingShingle | Betelihem Yirdaw Temesgen Kassa Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae) Veterinary Medicine and Science antimicrobial effects inhibition zone phytochemical plant |
| title | Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae) |
| title_full | Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae) |
| title_fullStr | Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae) |
| title_full_unstemmed | Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae) |
| title_short | Preliminary phytochemical screening and antibacterial effects of root bark of Ferula communis (Apiaceae) |
| title_sort | preliminary phytochemical screening and antibacterial effects of root bark of ferula communis apiaceae |
| topic | antimicrobial effects inhibition zone phytochemical plant |
| url | https://doi.org/10.1002/vms3.1170 |
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