Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus.
Since gene expression approaches constitute a starting point for investigating plant-pathogen systems, we performed a transcriptional analysis to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and Tomato Mosaic Virus (ToMV). Differentially ex...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4012952?pdf=render |
| _version_ | 1818512618765156352 |
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| author | Giuseppe Andolfo Francesca Ferriello Luca Tardella Alberto Ferrarini Loredana Sigillo Luigi Frusciante Maria Raffaella Ercolano |
| author_facet | Giuseppe Andolfo Francesca Ferriello Luca Tardella Alberto Ferrarini Loredana Sigillo Luigi Frusciante Maria Raffaella Ercolano |
| author_sort | Giuseppe Andolfo |
| collection | DOAJ |
| description | Since gene expression approaches constitute a starting point for investigating plant-pathogen systems, we performed a transcriptional analysis to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and Tomato Mosaic Virus (ToMV). Differentially expressed tomato genes upon inoculation with Fol and ToMV were identified at two days post-inoculation. A large overlap was found in differentially expressed genes throughout the two incompatible interactions. However, Gene Ontology enrichment analysis evidenced specific categories in both interactions. Response to ToMV seems more multifaceted, since more than 70 specific categories were enriched versus the 30 detected in Fol interaction. In particular, the virus stimulated the production of an invertase enzyme that is able to redirect the flux of carbohydrates, whereas Fol induced a homeostatic response to prevent the fungus from killing cells. Genomic mapping of transcripts suggested that specific genomic regions are involved in resistance response to pathogen. Coordinated machinery could play an important role in prompting the response, since 60% of pathogen receptor genes (NB-ARC-LRR, RLP, RLK) were differentially regulated during both interactions. Assessment of genomic gene expression patterns could help in building up models of mediated resistance responses. |
| first_indexed | 2024-12-10T23:49:28Z |
| format | Article |
| id | doaj.art-c451f80555964cc6a16791b387b9ae0e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T23:49:28Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c451f80555964cc6a16791b387b9ae0e2022-12-22T01:28:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9496310.1371/journal.pone.0094963Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus.Giuseppe AndolfoFrancesca FerrielloLuca TardellaAlberto FerrariniLoredana SigilloLuigi FruscianteMaria Raffaella ErcolanoSince gene expression approaches constitute a starting point for investigating plant-pathogen systems, we performed a transcriptional analysis to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and Tomato Mosaic Virus (ToMV). Differentially expressed tomato genes upon inoculation with Fol and ToMV were identified at two days post-inoculation. A large overlap was found in differentially expressed genes throughout the two incompatible interactions. However, Gene Ontology enrichment analysis evidenced specific categories in both interactions. Response to ToMV seems more multifaceted, since more than 70 specific categories were enriched versus the 30 detected in Fol interaction. In particular, the virus stimulated the production of an invertase enzyme that is able to redirect the flux of carbohydrates, whereas Fol induced a homeostatic response to prevent the fungus from killing cells. Genomic mapping of transcripts suggested that specific genomic regions are involved in resistance response to pathogen. Coordinated machinery could play an important role in prompting the response, since 60% of pathogen receptor genes (NB-ARC-LRR, RLP, RLK) were differentially regulated during both interactions. Assessment of genomic gene expression patterns could help in building up models of mediated resistance responses.http://europepmc.org/articles/PMC4012952?pdf=render |
| spellingShingle | Giuseppe Andolfo Francesca Ferriello Luca Tardella Alberto Ferrarini Loredana Sigillo Luigi Frusciante Maria Raffaella Ercolano Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus. PLoS ONE |
| title | Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus. |
| title_full | Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus. |
| title_fullStr | Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus. |
| title_full_unstemmed | Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus. |
| title_short | Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus. |
| title_sort | tomato genome wide transcriptional responses to fusarium wilt and tomato mosaic virus |
| url | http://europepmc.org/articles/PMC4012952?pdf=render |
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