Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought
Canola is an important temperate oil crop that can be severely affected by drought. Understanding the physiological and molecular mechanisms involved in canola tolerance to water deficit is essential to obtain drought-tolerant productive cultivars. To investigate the role of antioxidant response and...
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MDPI AG
2022-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/1/125 |
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| author | Hossein Ahmadi Alireza Abbasi Alireza Taleei Valiollah Mohammadi José J. Pueyo |
| author_facet | Hossein Ahmadi Alireza Abbasi Alireza Taleei Valiollah Mohammadi José J. Pueyo |
| author_sort | Hossein Ahmadi |
| collection | DOAJ |
| description | Canola is an important temperate oil crop that can be severely affected by drought. Understanding the physiological and molecular mechanisms involved in canola tolerance to water deficit is essential to obtain drought-tolerant productive cultivars. To investigate the role of antioxidant response and the possible involvement of calcium-dependent protein kinases (CDPKs) in canola tolerance to drought, we analyzed four genotypes with different sensitivity to water stress. Leaf relative water content, canopy temperature, PSII efficiency, electrolyte leakage index and lipid peroxidation were used as indicators to classify the cultivars as drought-tolerant or drought-sensitive. Antioxidant enzymes superoxide dismutase, guaiacol peroxidase and catalase displayed significantly higher activities in drought-tolerant than in drought-sensitive cultivars subjected to water deficit, suggesting that the efficiency of the antioxidant response is essential in canola drought tolerance. The increased expression of genes <i>BnaCDPK6</i> and <i>BnaCDPK14</i> under drought conditions, their differential expression in drought-tolerant and drought-sensitive genotypes, and the presence of multiple cis-acting stress-related elements in their promoter regions suggest that CDPKs are part of the signaling pathways that regulate drought response in canola. We propose the <i>BnaCDPK</i> genes and their regulator elements as potential molecular targets to obtain drought-tolerant productive canola cultivars through breeding or genetic transformation. |
| first_indexed | 2024-03-10T03:06:56Z |
| format | Article |
| id | doaj.art-c452a057fa624fa294efe6031a4c564c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:06:56Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c452a057fa624fa294efe6031a4c564c2023-11-23T12:38:41ZengMDPI AGAgronomy2073-43952022-01-0112112510.3390/agronomy12010125Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to DroughtHossein Ahmadi0Alireza Abbasi1Alireza Taleei2Valiollah Mohammadi3José J. Pueyo4Department of Agronomy and Plant Breeding, University of Tehran, Karaj 77871-31587, IranDepartment of Agronomy and Plant Breeding, University of Tehran, Karaj 77871-31587, IranDepartment of Agronomy and Plant Breeding, University of Tehran, Karaj 77871-31587, IranDepartment of Agronomy and Plant Breeding, University of Tehran, Karaj 77871-31587, IranInstitute of Agricultural Sciences, ICA-CSIC, 28006 Madrid, SpainCanola is an important temperate oil crop that can be severely affected by drought. Understanding the physiological and molecular mechanisms involved in canola tolerance to water deficit is essential to obtain drought-tolerant productive cultivars. To investigate the role of antioxidant response and the possible involvement of calcium-dependent protein kinases (CDPKs) in canola tolerance to drought, we analyzed four genotypes with different sensitivity to water stress. Leaf relative water content, canopy temperature, PSII efficiency, electrolyte leakage index and lipid peroxidation were used as indicators to classify the cultivars as drought-tolerant or drought-sensitive. Antioxidant enzymes superoxide dismutase, guaiacol peroxidase and catalase displayed significantly higher activities in drought-tolerant than in drought-sensitive cultivars subjected to water deficit, suggesting that the efficiency of the antioxidant response is essential in canola drought tolerance. The increased expression of genes <i>BnaCDPK6</i> and <i>BnaCDPK14</i> under drought conditions, their differential expression in drought-tolerant and drought-sensitive genotypes, and the presence of multiple cis-acting stress-related elements in their promoter regions suggest that CDPKs are part of the signaling pathways that regulate drought response in canola. We propose the <i>BnaCDPK</i> genes and their regulator elements as potential molecular targets to obtain drought-tolerant productive canola cultivars through breeding or genetic transformation.https://www.mdpi.com/2073-4395/12/1/125<i>Brassica napus</i>canolarapeseeddroughtcalcium-dependent protein kinases<i>CDPK</i> |
| spellingShingle | Hossein Ahmadi Alireza Abbasi Alireza Taleei Valiollah Mohammadi José J. Pueyo Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought Agronomy <i>Brassica napus</i> canola rapeseed drought calcium-dependent protein kinases <i>CDPK</i> |
| title | Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought |
| title_full | Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought |
| title_fullStr | Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought |
| title_full_unstemmed | Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought |
| title_short | Antioxidant Response and Calcium-Dependent Protein Kinases Involvement in Canola (<i>Brassica napus</i> L.) Tolerance to Drought |
| title_sort | antioxidant response and calcium dependent protein kinases involvement in canola i brassica napus i l tolerance to drought |
| topic | <i>Brassica napus</i> canola rapeseed drought calcium-dependent protein kinases <i>CDPK</i> |
| url | https://www.mdpi.com/2073-4395/12/1/125 |
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