Wort disinfection treatment with electron beam for bioethanol production
ABSTRACT Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort f...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-06-01
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| Series: | Scientia Agricola |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100102&tlng=en |
| _version_ | 1811342613298544640 |
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| author | Rubens Perez Calegari Eric Alberto da Silva Ana Paula Maria da Silva Marcelo Pego Gomes Layna Amorim Mota Valter Arthur Antonio Sampaio Baptista |
| author_facet | Rubens Perez Calegari Eric Alberto da Silva Ana Paula Maria da Silva Marcelo Pego Gomes Layna Amorim Mota Valter Arthur Antonio Sampaio Baptista |
| author_sort | Rubens Perez Calegari |
| collection | DOAJ |
| description | ABSTRACT Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort from sugarcane (Saccharum officinarum L.) molasses and investigate the bioethanol fermentation. Four treatments (T0 – T3) were carried out using ionizing doses of radiation through the electron accelerator: 0 (control), 10, 20, and 40 kGy. Total mesophiles, total bacteria, sucrose, glucose, fructose, phenolics, flavonoids, hydroxymethylfurfural (5-HMF), and Furfural were measured. An alcoholic fermentation assay was performed after the irradiation process. The irradiated treatments showed no inversion of sugars and formation of the inhibitory by-products flavonoids, furfural and 5-HMF, except for the phenolic compounds. The lower dose tested (10 kGy) reduced more than 99.9 % of the total mesophiles and more than 99.99 % of the total bacteria in the substrate. In the fermentation, the irradiated worts presented similar (p > 0.05) yields (92, 93, and 94 %) and ethanol productivity levels (0.89, 0.88, and 0.87 g L–1 h–1, for T1, T2, and T3 respectively). However, all treatments presented higher yields and productivity (p < 0.05) when compared to the control (88 % and 0.85 g L–1 h–1), highlighting the possible use of e-beam in wort fermentation at a lower dose (10 kGy). This allows reduction in losses caused by microbial contamination, besides increasing fermentation yield and productivity with lower energy consumption. |
| first_indexed | 2024-04-13T19:15:02Z |
| format | Article |
| id | doaj.art-c4538b6f40fb4f24b414bdc2f00738eb |
| institution | Directory Open Access Journal |
| issn | 1678-992X |
| language | English |
| last_indexed | 2024-04-13T19:15:02Z |
| publishDate | 2022-06-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Scientia Agricola |
| spelling | doaj.art-c4538b6f40fb4f24b414bdc2f00738eb2022-12-22T02:33:43ZengUniversidade de São PauloScientia Agricola1678-992X2022-06-018010.1590/1678-992x-2021-0260Wort disinfection treatment with electron beam for bioethanol productionRubens Perez Calegarihttps://orcid.org/0000-0002-9929-4736Eric Alberto da Silvahttps://orcid.org/0000-0003-3127-2941Ana Paula Maria da Silvahttps://orcid.org/0000-0002-1486-9853Marcelo Pego Gomeshttps://orcid.org/0000-0002-3051-9714Layna Amorim Motahttps://orcid.org/0000-0002-7684-3958Valter Arthurhttps://orcid.org/0000-0003-3521-9136Antonio Sampaio Baptistahttps://orcid.org/0000-0001-5283-2034ABSTRACT Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort from sugarcane (Saccharum officinarum L.) molasses and investigate the bioethanol fermentation. Four treatments (T0 – T3) were carried out using ionizing doses of radiation through the electron accelerator: 0 (control), 10, 20, and 40 kGy. Total mesophiles, total bacteria, sucrose, glucose, fructose, phenolics, flavonoids, hydroxymethylfurfural (5-HMF), and Furfural were measured. An alcoholic fermentation assay was performed after the irradiation process. The irradiated treatments showed no inversion of sugars and formation of the inhibitory by-products flavonoids, furfural and 5-HMF, except for the phenolic compounds. The lower dose tested (10 kGy) reduced more than 99.9 % of the total mesophiles and more than 99.99 % of the total bacteria in the substrate. In the fermentation, the irradiated worts presented similar (p > 0.05) yields (92, 93, and 94 %) and ethanol productivity levels (0.89, 0.88, and 0.87 g L–1 h–1, for T1, T2, and T3 respectively). However, all treatments presented higher yields and productivity (p < 0.05) when compared to the control (88 % and 0.85 g L–1 h–1), highlighting the possible use of e-beam in wort fermentation at a lower dose (10 kGy). This allows reduction in losses caused by microbial contamination, besides increasing fermentation yield and productivity with lower energy consumption.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100102&tlng=enalcoholic fermentationbiofuelelectron acceleratormicrobial contaminationreduction of contamination |
| spellingShingle | Rubens Perez Calegari Eric Alberto da Silva Ana Paula Maria da Silva Marcelo Pego Gomes Layna Amorim Mota Valter Arthur Antonio Sampaio Baptista Wort disinfection treatment with electron beam for bioethanol production Scientia Agricola alcoholic fermentation biofuel electron accelerator microbial contamination reduction of contamination |
| title | Wort disinfection treatment with electron beam for bioethanol production |
| title_full | Wort disinfection treatment with electron beam for bioethanol production |
| title_fullStr | Wort disinfection treatment with electron beam for bioethanol production |
| title_full_unstemmed | Wort disinfection treatment with electron beam for bioethanol production |
| title_short | Wort disinfection treatment with electron beam for bioethanol production |
| title_sort | wort disinfection treatment with electron beam for bioethanol production |
| topic | alcoholic fermentation biofuel electron accelerator microbial contamination reduction of contamination |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100102&tlng=en |
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