Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum)
Abstract The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was mea...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Laboratory Animal Research |
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| Online Access: | http://link.springer.com/article/10.5625/lar.2017.33.1.32 |
| _version_ | 1811210658161623040 |
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| author | Joo-Hee Choi Sung-Gang Jo Seoung-Ki Jung Woo-Tae Park Keun-Young Kim Yong-Wook Park Jong-Hwan Park |
| author_facet | Joo-Hee Choi Sung-Gang Jo Seoung-Ki Jung Woo-Tae Park Keun-Young Kim Yong-Wook Park Jong-Hwan Park |
| author_sort | Joo-Hee Choi |
| collection | DOAJ |
| description | Abstract The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-α production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNγ. In vivo studies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement. |
| first_indexed | 2024-04-12T04:59:31Z |
| format | Article |
| id | doaj.art-c45dee6022c94836b6fcc72186c49301 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-04-12T04:59:31Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-c45dee6022c94836b6fcc72186c493012022-12-22T03:47:02ZengBMCLaboratory Animal Research2233-76602017-12-01331323910.5625/lar.2017.33.1.32Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum)Joo-Hee Choi0Sung-Gang Jo1Seoung-Ki Jung2Woo-Tae Park3Keun-Young Kim4Yong-Wook Park5Jong-Hwan Park6Laboratory Animal Medicine, College of Veterinary Medicine and Brain Korea 21 PLUS Project Team, Chonnam National UniversityLaboratory Animal Medicine, College of Veterinary Medicine and Brain Korea 21 PLUS Project Team, Chonnam National UniversityInstitute of Biotechnology, Bioresource Inc., Cheomdan ventureInstitute of Biotechnology, Bioresource Inc., Cheomdan ventureInstitute of Biotechnology, Bioresource Inc., Cheomdan ventureDepartment of Rheumatology, Chonnam National University Medical School, Chonnam National University HospitalLaboratory Animal Medicine, College of Veterinary Medicine and Brain Korea 21 PLUS Project Team, Chonnam National UniversityAbstract The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-α production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNγ. In vivo studies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement.http://link.springer.com/article/10.5625/lar.2017.33.1.32Ice plant extract (IPE)lymphocytemacrophagecytokinescell proliferation |
| spellingShingle | Joo-Hee Choi Sung-Gang Jo Seoung-Ki Jung Woo-Tae Park Keun-Young Kim Yong-Wook Park Jong-Hwan Park Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum) Laboratory Animal Research Ice plant extract (IPE) lymphocyte macrophage cytokines cell proliferation |
| title | Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum) |
| title_full | Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum) |
| title_fullStr | Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum) |
| title_full_unstemmed | Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum) |
| title_short | Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum) |
| title_sort | immunomodulatory effects of ethanol extract of germinated ice plant mesembryanthemum crystallinum |
| topic | Ice plant extract (IPE) lymphocyte macrophage cytokines cell proliferation |
| url | http://link.springer.com/article/10.5625/lar.2017.33.1.32 |
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