Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion
Ischemia/reperfusion (I/R) injury is a common pathological mechanism involved in many ocular diseases. I/R is characterized by microvascular dysfunction and neurodegeneration. However, the mechanisms of neurodegeneration induced by I/R remain largely unknown. This study showed that the expression of...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022022824 |
| _version_ | 1811247907833118720 |
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| author | Ting-Ting Sun Xiu-Miao Li Jun-Ya Zhu Wen Yao Tian-Jing Yang Xiang-Rui Meng Jin Yao Qin Jiang |
| author_facet | Ting-Ting Sun Xiu-Miao Li Jun-Ya Zhu Wen Yao Tian-Jing Yang Xiang-Rui Meng Jin Yao Qin Jiang |
| author_sort | Ting-Ting Sun |
| collection | DOAJ |
| description | Ischemia/reperfusion (I/R) injury is a common pathological mechanism involved in many ocular diseases. I/R is characterized by microvascular dysfunction and neurodegeneration. However, the mechanisms of neurodegeneration induced by I/R remain largely unknown. This study showed that the expression of long non-coding RNA-CRNDE was significantly upregulated after retinal ischemia-reperfusion (RIR). LncRNA-CRNDE knockdown alleviated retinal neurodegeneration induced by RIR injury, as shown by decreased reactive gliosis and reduced retinal cells loss. Furthermore, lncRNA-CRNDE knockdown directly regulated Müller cell function and indirectly affected RGC function in vitro. In addition, lncRNA-CRNDE knockdown led to a significant reduction in the release of several cytokines after RIR. This study suggests that lncRNA-CRNDE is a promising therapeutic target for RIR. |
| first_indexed | 2024-04-12T15:18:49Z |
| format | Article |
| id | doaj.art-c45ef7a8ec9645dc9978628839cc549f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T15:18:49Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c45ef7a8ec9645dc9978628839cc549f2022-12-22T03:27:33ZengElsevierHeliyon2405-84402022-10-01810e10994Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusionTing-Ting Sun0Xiu-Miao Li1Jun-Ya Zhu2Wen Yao3Tian-Jing Yang4Xiang-Rui Meng5Jin Yao6Qin Jiang7The Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, ChinaThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, ChinaThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, ChinaThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, ChinaThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, ChinaFaculty of Art and Science, Queens University, Kingston, Ontario, CanadaThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China; Corresponding author.The Affiliated Eye Hospital, Nanjing Medical University, Nanjing, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China; Corresponding author.Ischemia/reperfusion (I/R) injury is a common pathological mechanism involved in many ocular diseases. I/R is characterized by microvascular dysfunction and neurodegeneration. However, the mechanisms of neurodegeneration induced by I/R remain largely unknown. This study showed that the expression of long non-coding RNA-CRNDE was significantly upregulated after retinal ischemia-reperfusion (RIR). LncRNA-CRNDE knockdown alleviated retinal neurodegeneration induced by RIR injury, as shown by decreased reactive gliosis and reduced retinal cells loss. Furthermore, lncRNA-CRNDE knockdown directly regulated Müller cell function and indirectly affected RGC function in vitro. In addition, lncRNA-CRNDE knockdown led to a significant reduction in the release of several cytokines after RIR. This study suggests that lncRNA-CRNDE is a promising therapeutic target for RIR.http://www.sciencedirect.com/science/article/pii/S2405844022022824Retinal ischemia-reperfusion injuryLong non-coding RNARetinal neurodegeneration |
| spellingShingle | Ting-Ting Sun Xiu-Miao Li Jun-Ya Zhu Wen Yao Tian-Jing Yang Xiang-Rui Meng Jin Yao Qin Jiang Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion Heliyon Retinal ischemia-reperfusion injury Long non-coding RNA Retinal neurodegeneration |
| title | Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion |
| title_full | Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion |
| title_fullStr | Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion |
| title_full_unstemmed | Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion |
| title_short | Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion |
| title_sort | regulatory effect of long stranded non coding rna crnde on neurodegeneration during retinal ischemia reperfusion |
| topic | Retinal ischemia-reperfusion injury Long non-coding RNA Retinal neurodegeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022022824 |
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