Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms
Abstract Background To investigate the effect of ficin, a type of proteases, on Candida albicans (C. albicans) biofilm, including forming and pre-formed biofilms. Methods Crystal violet tests together with colony forming unit (CFU) counts were used to detect fungal biofilm biomass. Live/dead stainin...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-08-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-022-02384-y |
| _version_ | 1811215383849336832 |
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| author | Jiantao Yu Fan Wang Yan Shen Fangzheng Yu Lili Qiu Lingjun Zhang Yanhan Chen Qing Yuan Huan Zhang Yan Sun Keke Zhang |
| author_facet | Jiantao Yu Fan Wang Yan Shen Fangzheng Yu Lili Qiu Lingjun Zhang Yanhan Chen Qing Yuan Huan Zhang Yan Sun Keke Zhang |
| author_sort | Jiantao Yu |
| collection | DOAJ |
| description | Abstract Background To investigate the effect of ficin, a type of proteases, on Candida albicans (C. albicans) biofilm, including forming and pre-formed biofilms. Methods Crystal violet tests together with colony forming unit (CFU) counts were used to detect fungal biofilm biomass. Live/dead staining of biofilms observed by confocal laser scanning microscopy was used to monitor fungal activity. Finally, gene expression of C. albicans within biofilms was assessed by qRT-PCR. Results According to our results, biofilm biomass was dramatically reduced by ficin in both biofilm formation and pre-formed biofilms, as revealed by the crystal violet assay and CFU count (p < 0.05). Fungal activity in biofilm formation and pre-formed biofilms was not significantly influenced by ficin according to live/dead staining. Fungal polymorphism and biofilm associated gene expression were influenced by ficin, especially in groups with prominent antibiofilm effects. Conclusions In summary, ficin effectively inhibited C. albicans biofilm formation and detached its preformed biofilm, and it might be used to treat C. albicans biofilm associated problems. |
| first_indexed | 2024-04-12T06:21:45Z |
| format | Article |
| id | doaj.art-c45fb2bdd97143d797f078a228625e28 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-12T06:21:45Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-c45fb2bdd97143d797f078a228625e282022-12-22T03:44:17ZengBMCBMC Oral Health1472-68312022-08-012211810.1186/s12903-022-02384-yInhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilmsJiantao Yu0Fan Wang1Yan Shen2Fangzheng Yu3Lili Qiu4Lingjun Zhang5Yanhan Chen6Qing Yuan7Huan Zhang8Yan Sun9Keke Zhang10School and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversitySchool and Hospital of Stomatology, Wenzhou Medical UniversityAbstract Background To investigate the effect of ficin, a type of proteases, on Candida albicans (C. albicans) biofilm, including forming and pre-formed biofilms. Methods Crystal violet tests together with colony forming unit (CFU) counts were used to detect fungal biofilm biomass. Live/dead staining of biofilms observed by confocal laser scanning microscopy was used to monitor fungal activity. Finally, gene expression of C. albicans within biofilms was assessed by qRT-PCR. Results According to our results, biofilm biomass was dramatically reduced by ficin in both biofilm formation and pre-formed biofilms, as revealed by the crystal violet assay and CFU count (p < 0.05). Fungal activity in biofilm formation and pre-formed biofilms was not significantly influenced by ficin according to live/dead staining. Fungal polymorphism and biofilm associated gene expression were influenced by ficin, especially in groups with prominent antibiofilm effects. Conclusions In summary, ficin effectively inhibited C. albicans biofilm formation and detached its preformed biofilm, and it might be used to treat C. albicans biofilm associated problems.https://doi.org/10.1186/s12903-022-02384-yCandida albicansFicinBiofilm formationPre-formed biofilm |
| spellingShingle | Jiantao Yu Fan Wang Yan Shen Fangzheng Yu Lili Qiu Lingjun Zhang Yanhan Chen Qing Yuan Huan Zhang Yan Sun Keke Zhang Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms BMC Oral Health Candida albicans Ficin Biofilm formation Pre-formed biofilm |
| title | Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms |
| title_full | Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms |
| title_fullStr | Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms |
| title_full_unstemmed | Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms |
| title_short | Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms |
| title_sort | inhibitory effect of ficin on candida albicans biofilm formation and pre formed biofilms |
| topic | Candida albicans Ficin Biofilm formation Pre-formed biofilm |
| url | https://doi.org/10.1186/s12903-022-02384-y |
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