Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress
Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germ-plasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objecti...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2014-01-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/175/188 |
| _version_ | 1797641637496619008 |
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| author | Aminatun Munawarti Taryono Endang Semiarti Sismindari |
| author_facet | Aminatun Munawarti Taryono Endang Semiarti Sismindari |
| author_sort | Aminatun Munawarti |
| collection | DOAJ |
| description | Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germ-plasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on morphological (plant height, stalk diameter, green leaf number) and biochemical (leaf proline content and protein pattern) responses of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two-month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54 |
| first_indexed | 2024-03-11T13:48:33Z |
| format | Article |
| id | doaj.art-c460eed20afa47b4b52716805440ae25 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-11T13:48:33Z |
| publishDate | 2014-01-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-c460eed20afa47b4b52716805440ae252023-11-02T09:40:42ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172014-01-01416166Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought StressAminatun Munawarti0Taryono1Endang Semiarti2Sismindari3Postgraduate Program of Biotechnology, Gadjah Mada University, Yogyakarta, Indonesia, Department of Biology, Faculty of MIPA, Brawijaya University, Malang, IndonesiaDepartment of Agri cultural Production Technology, Faculty of Agriculture, Gadjah Mada University Yogyakarta, IndonesiaFacu l ty of Biology, Gadjah Mada University, Yogyakarta, IndonesiaFaculty of Pharmacy, Gadjah Mada University, Yogyakarta, IndonesiaGlagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germ-plasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on morphological (plant height, stalk diameter, green leaf number) and biochemical (leaf proline content and protein pattern) responses of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two-month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/175/188Drought stressglagahprolineprotein patternSaccharum spontaneum |
| spellingShingle | Aminatun Munawarti Taryono Endang Semiarti Sismindari Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress Journal of Tropical Life Science Drought stress glagah proline protein pattern Saccharum spontaneum |
| title | Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress |
| title_full | Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress |
| title_fullStr | Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress |
| title_full_unstemmed | Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress |
| title_short | Morphological and Biochemical Responses of Glagah ( Saccharum spontaneum L.) Accessions to Drought Stress |
| title_sort | morphological and biochemical responses of glagah saccharum spontaneum l accessions to drought stress |
| topic | Drought stress glagah proline protein pattern Saccharum spontaneum |
| url | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/175/188 |
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