Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research
Abstract Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-86571-x |
| _version_ | 1818842076988571648 |
|---|---|
| author | Łukasz Richter Karolina Księżarczyk Karolina Paszkowska Marta Janczuk-Richter Joanna Niedziółka-Jönsson Jacek Gapiński Marcin Łoś Robert Hołyst Jan Paczesny |
| author_facet | Łukasz Richter Karolina Księżarczyk Karolina Paszkowska Marta Janczuk-Richter Joanna Niedziółka-Jönsson Jacek Gapiński Marcin Łoś Robert Hołyst Jan Paczesny |
| author_sort | Łukasz Richter |
| collection | DOAJ |
| description | Abstract Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology. |
| first_indexed | 2024-12-19T04:36:14Z |
| format | Article |
| id | doaj.art-c466dbe6c3284005b81ea654db2a385c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:36:14Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c466dbe6c3284005b81ea654db2a385c2022-12-21T20:35:45ZengNature PortfolioScientific Reports2045-23222021-04-0111111210.1038/s41598-021-86571-xAdsorption of bacteriophages on polypropylene labware affects the reproducibility of phage researchŁukasz Richter0Karolina Księżarczyk1Karolina Paszkowska2Marta Janczuk-Richter3Joanna Niedziółka-Jönsson4Jacek Gapiński5Marcin Łoś6Robert Hołyst7Jan Paczesny8Institute of Physical Chemistry, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesDepartment of Molecular Biophysics, Adam Mickiewicz University in PoznańDepartment of Molecular Biology, University of GdańskInstitute of Physical Chemistry, Polish Academy of SciencesInstitute of Physical Chemistry, Polish Academy of SciencesAbstract Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.https://doi.org/10.1038/s41598-021-86571-x |
| spellingShingle | Łukasz Richter Karolina Księżarczyk Karolina Paszkowska Marta Janczuk-Richter Joanna Niedziółka-Jönsson Jacek Gapiński Marcin Łoś Robert Hołyst Jan Paczesny Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research Scientific Reports |
| title | Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research |
| title_full | Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research |
| title_fullStr | Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research |
| title_full_unstemmed | Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research |
| title_short | Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research |
| title_sort | adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research |
| url | https://doi.org/10.1038/s41598-021-86571-x |
| work_keys_str_mv | AT łukaszrichter adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT karolinaksiezarczyk adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT karolinapaszkowska adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT martajanczukrichter adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT joannaniedziołkajonsson adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT jacekgapinski adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT marcinłos adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT roberthołyst adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch AT janpaczesny adsorptionofbacteriophagesonpolypropylenelabwareaffectsthereproducibilityofphageresearch |