Diet–Microbiota Interactions Alter Mosquito Development
Gut microbes and diet can both strongly affect the biology of multicellular animals, but it is often difficult to disentangle microbiota–diet interactions due to the complex microbial communities many animals harbor and the nutritionally variable diets they consume. While theoretical and empirical s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.650743/full |
| _version_ | 1818779260149563392 |
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| author | Vincent G. Martinson Vincent G. Martinson Michael R. Strand |
| author_facet | Vincent G. Martinson Vincent G. Martinson Michael R. Strand |
| author_sort | Vincent G. Martinson |
| collection | DOAJ |
| description | Gut microbes and diet can both strongly affect the biology of multicellular animals, but it is often difficult to disentangle microbiota–diet interactions due to the complex microbial communities many animals harbor and the nutritionally variable diets they consume. While theoretical and empirical studies indicate that greater microbiota diversity is beneficial for many animal hosts, there have been few tests performed in aquatic invertebrates. Most mosquito species are aquatic detritivores during their juvenile stages that harbor variable microbiotas and consume diets that range from nutrient rich to nutrient poor. In this study, we produced a gnotobiotic model that allowed us to examine how interactions between specific gut microbes and diets affect the fitness of Aedes aegypti, the yellow fever mosquito. Using a simplified seven-member community of bacteria (ALL7) and various laboratory and natural mosquito diets, we allowed larval mosquitoes to develop under different microbial and dietary conditions and measured the resulting time to adulthood and adult size. Larvae inoculated with the ALL7 or a more complex community developed similarly when fed nutrient-rich rat chow or fish food laboratory diets, whereas larvae inoculated with individual bacterial members of the ALL7 community exhibited few differences in development when fed a rat chow diet but exhibited large differences in performance when fed a fish food diet. In contrast, the ALL7 community largely failed to support the growth of larvae fed field-collected detritus diets unless supplemented with additional protein or yeast. Collectively, our results indicate that mosquito development and fitness are strongly contingent on both diet and microbial community composition. |
| first_indexed | 2024-12-18T11:57:47Z |
| format | Article |
| id | doaj.art-c4677d81a7d34e12904e04680177115a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-18T11:57:47Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c4677d81a7d34e12904e04680177115a2022-12-21T21:09:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.650743650743Diet–Microbiota Interactions Alter Mosquito DevelopmentVincent G. Martinson0Vincent G. Martinson1Michael R. Strand2Department of Entomology, University of Georgia, Athens, GA, United StatesDepartment of Biology, University of New Mexico, Albuquerque, NM, United StatesDepartment of Entomology, University of Georgia, Athens, GA, United StatesGut microbes and diet can both strongly affect the biology of multicellular animals, but it is often difficult to disentangle microbiota–diet interactions due to the complex microbial communities many animals harbor and the nutritionally variable diets they consume. While theoretical and empirical studies indicate that greater microbiota diversity is beneficial for many animal hosts, there have been few tests performed in aquatic invertebrates. Most mosquito species are aquatic detritivores during their juvenile stages that harbor variable microbiotas and consume diets that range from nutrient rich to nutrient poor. In this study, we produced a gnotobiotic model that allowed us to examine how interactions between specific gut microbes and diets affect the fitness of Aedes aegypti, the yellow fever mosquito. Using a simplified seven-member community of bacteria (ALL7) and various laboratory and natural mosquito diets, we allowed larval mosquitoes to develop under different microbial and dietary conditions and measured the resulting time to adulthood and adult size. Larvae inoculated with the ALL7 or a more complex community developed similarly when fed nutrient-rich rat chow or fish food laboratory diets, whereas larvae inoculated with individual bacterial members of the ALL7 community exhibited few differences in development when fed a rat chow diet but exhibited large differences in performance when fed a fish food diet. In contrast, the ALL7 community largely failed to support the growth of larvae fed field-collected detritus diets unless supplemented with additional protein or yeast. Collectively, our results indicate that mosquito development and fitness are strongly contingent on both diet and microbial community composition.https://www.frontiersin.org/articles/10.3389/fmicb.2021.650743/fullAedes aegyptimicrobiota diversityhost–microbiotamicrobe–microbediet–microbe |
| spellingShingle | Vincent G. Martinson Vincent G. Martinson Michael R. Strand Diet–Microbiota Interactions Alter Mosquito Development Frontiers in Microbiology Aedes aegypti microbiota diversity host–microbiota microbe–microbe diet–microbe |
| title | Diet–Microbiota Interactions Alter Mosquito Development |
| title_full | Diet–Microbiota Interactions Alter Mosquito Development |
| title_fullStr | Diet–Microbiota Interactions Alter Mosquito Development |
| title_full_unstemmed | Diet–Microbiota Interactions Alter Mosquito Development |
| title_short | Diet–Microbiota Interactions Alter Mosquito Development |
| title_sort | diet microbiota interactions alter mosquito development |
| topic | Aedes aegypti microbiota diversity host–microbiota microbe–microbe diet–microbe |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.650743/full |
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