miRNA profiling in intrauterine exosomes of pregnant cattle on day 7
Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recip...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1078394/full |
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| author | Yaying Zhai Yaying Zhai Qiaoting Shi Qiuxia Chu Fuying Chen Yajie Feng Zijing Zhang Xinglei Qi Danny Arends Gudrun A. Brockmann Eryao Wang Shijie Lyu Shijie Lyu |
| author_facet | Yaying Zhai Yaying Zhai Qiaoting Shi Qiuxia Chu Fuying Chen Yajie Feng Zijing Zhang Xinglei Qi Danny Arends Gudrun A. Brockmann Eryao Wang Shijie Lyu Shijie Lyu |
| author_sort | Yaying Zhai |
| collection | DOAJ |
| description | Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (p-value < 0.05, |log2(FoldChange)| > 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (PBenjamini-Hochberg < 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation. |
| first_indexed | 2024-04-11T05:49:15Z |
| format | Article |
| id | doaj.art-c468af0cbdc9456681596228fb1b0a92 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T05:49:15Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-c468af0cbdc9456681596228fb1b0a922022-12-22T04:42:08ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-12-01910.3389/fvets.2022.10783941078394miRNA profiling in intrauterine exosomes of pregnant cattle on day 7Yaying Zhai0Yaying Zhai1Qiaoting Shi2Qiuxia Chu3Fuying Chen4Yajie Feng5Zijing Zhang6Xinglei Qi7Danny Arends8Gudrun A. Brockmann9Eryao Wang10Shijie Lyu11Shijie Lyu12Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaCenter of Animal Husbandry Technical Service in Biyang, Zhumadian, ChinaDepartment of Applied Sciences, Northumbria University, Newcastle upon Tyne, United KingdomAlbrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Berlin, GermanyInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaInstitute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, ChinaThe Shennong Laboratory, Zhengzhou, Henan, ChinaIntrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (p-value < 0.05, |log2(FoldChange)| > 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (PBenjamini-Hochberg < 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation.https://www.frontiersin.org/articles/10.3389/fvets.2022.1078394/fullbovineuterine flushingreceptive endometriumapoptosisproliferation |
| spellingShingle | Yaying Zhai Yaying Zhai Qiaoting Shi Qiuxia Chu Fuying Chen Yajie Feng Zijing Zhang Xinglei Qi Danny Arends Gudrun A. Brockmann Eryao Wang Shijie Lyu Shijie Lyu miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 Frontiers in Veterinary Science bovine uterine flushing receptive endometrium apoptosis proliferation |
| title | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
| title_full | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
| title_fullStr | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
| title_full_unstemmed | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
| title_short | miRNA profiling in intrauterine exosomes of pregnant cattle on day 7 |
| title_sort | mirna profiling in intrauterine exosomes of pregnant cattle on day 7 |
| topic | bovine uterine flushing receptive endometrium apoptosis proliferation |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1078394/full |
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