Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids
Objective(s):The inhibitory effects of iron chelators, and FeCl3 chelation on biofilm formation and swarming motility were investigated against an opportunistic human pathogen Pseudomonas aeruginosa. Materials and Methods:The inhibitory activity of 2,2’-bipyridyl, lipoic acid, kojic acid and picolin...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2015-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_4726_bca48e854e39f5fe8906b93594d70418.html |
| _version_ | 1819052624262987776 |
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| author | Kübra Çevik Seyhan Ulusoy |
| author_facet | Kübra Çevik Seyhan Ulusoy |
| author_sort | Kübra Çevik |
| collection | DOAJ |
| description | Objective(s):The inhibitory effects of iron chelators, and FeCl3 chelation on biofilm formation and swarming motility were investigated against an opportunistic human pathogen Pseudomonas aeruginosa. Materials and Methods:The inhibitory activity of 2,2’-bipyridyl, lipoic acid, kojic acid and picolinic acidonbiofilm formation of P. aeruginosa strain PAO1 and three clinical isolates (P. aeruginosa PAK01,P. aeruginosa PAK02 and P. aeruginosa PAK03) were investigated, based on crystal violet assay, and swarming motility test. Results:The kojic, lipoic and picolinic acid inhibited biofilm formation by 5-33% in all tested P. aeruginosa isolates. When chelated iron was added, biofilm inhibition rates were determined to be 39-57%. Among the tested chelators against P. aeruginosa, lipoic acid (84%) and kojic acid (68%) presented the highest inhibition of swarming motility. This is the first study to report the inhibitory effect of lipoic acid on biofilm formation and swarming motility of P. aeruginosa. Conclusion: It is considered that lipoic and picolinic acids can serve as alternatives for the treatment of the P. aeruginosa infections by inhibiting biofilm formation. |
| first_indexed | 2024-12-21T12:22:47Z |
| format | Article |
| id | doaj.art-c46b15f4d91d42c6a4bbc80af6862764 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-21T12:22:47Z |
| publishDate | 2015-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c46b15f4d91d42c6a4bbc80af68627642022-12-21T19:04:16ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-08-011887587634726Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acidsKübra Çevik0Seyhan Ulusoy1Department of Biology, Suleyman Demirel University, 32260 Isparta, TürkiyeDepartment of Biology, Suleyman Demirel University, 32260 Isparta, TürkiyeObjective(s):The inhibitory effects of iron chelators, and FeCl3 chelation on biofilm formation and swarming motility were investigated against an opportunistic human pathogen Pseudomonas aeruginosa. Materials and Methods:The inhibitory activity of 2,2’-bipyridyl, lipoic acid, kojic acid and picolinic acidonbiofilm formation of P. aeruginosa strain PAO1 and three clinical isolates (P. aeruginosa PAK01,P. aeruginosa PAK02 and P. aeruginosa PAK03) were investigated, based on crystal violet assay, and swarming motility test. Results:The kojic, lipoic and picolinic acid inhibited biofilm formation by 5-33% in all tested P. aeruginosa isolates. When chelated iron was added, biofilm inhibition rates were determined to be 39-57%. Among the tested chelators against P. aeruginosa, lipoic acid (84%) and kojic acid (68%) presented the highest inhibition of swarming motility. This is the first study to report the inhibitory effect of lipoic acid on biofilm formation and swarming motility of P. aeruginosa. Conclusion: It is considered that lipoic and picolinic acids can serve as alternatives for the treatment of the P. aeruginosa infections by inhibiting biofilm formation.http://ijbms.mums.ac.ir/pdf_4726_bca48e854e39f5fe8906b93594d70418.htmlAlpha lipoic acidBiofilmKojic acidPicolinic acidPseudomonas aeruginosaSwarming motility |
| spellingShingle | Kübra Çevik Seyhan Ulusoy Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids Iranian Journal of Basic Medical Sciences Alpha lipoic acid Biofilm Kojic acid Picolinic acid Pseudomonas aeruginosa Swarming motility |
| title | Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids |
| title_full | Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids |
| title_fullStr | Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids |
| title_full_unstemmed | Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids |
| title_short | Inhibition of Pseudomonas aeruginosa biofilm formation by 2,2’-bipyridyl, lipoic, kojic and picolinic acids |
| title_sort | inhibition of pseudomonas aeruginosa biofilm formation by 2 2 bipyridyl lipoic kojic and picolinic acids |
| topic | Alpha lipoic acid Biofilm Kojic acid Picolinic acid Pseudomonas aeruginosa Swarming motility |
| url | http://ijbms.mums.ac.ir/pdf_4726_bca48e854e39f5fe8906b93594d70418.html |
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