Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells
Fumonisins (Fums) are types of mycotoxin that widely contaminante feed material crops, and can trigger potential biological toxicities to humans and various animals. However, the toxicity of Fums on porcine blood vessels has not been fully explored. Fumonisin B<sub>1</sub> (FB<sub>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/7/387 |
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| author | Qiaoling Yuan Yancheng Jiang Ying Fan Yingfeng Ma Hongyu Lei Jianming Su |
| author_facet | Qiaoling Yuan Yancheng Jiang Ying Fan Yingfeng Ma Hongyu Lei Jianming Su |
| author_sort | Qiaoling Yuan |
| collection | DOAJ |
| description | Fumonisins (Fums) are types of mycotoxin that widely contaminante feed material crops, and can trigger potential biological toxicities to humans and various animals. However, the toxicity of Fums on porcine blood vessels has not been fully explored. Fumonisin B<sub>1</sub> (FB<sub>1</sub>) is the main component of Fums. Therefore, the aim of this study was to explore the effects of FB<sub>1</sub> on the oxidative stress and tight junctions of the pig iliac endothelial cells (PIECs) in vitro. The results showed that FB<sub>1</sub> reduced the viability of PIECs, increased the contents of lipid peroxidation product malondialdehyde (MDA), decreased the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thioredoxin reductase (TrxR), and decreased the level of glutathione (GSH). In addition, the barrier functions were destroyed, along with the down-regulations on Claudin 1, Occludin and ZO-1 and the increase of paracellular permeability. Thus, this research indicates that FB<sub>1</sub> facilitates oxidative stress and breaks barrier functions to damage pig iliac endothelium cells. |
| first_indexed | 2024-04-11T22:21:20Z |
| format | Article |
| id | doaj.art-c46b6670d0904855bdc7d43e0a313470 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:21:20Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-c46b6670d0904855bdc7d43e0a3134702022-12-22T04:00:07ZengMDPI AGToxins2072-66512019-07-0111738710.3390/toxins11070387toxins11070387Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium CellsQiaoling Yuan0Yancheng Jiang1Ying Fan2Yingfeng Ma3Hongyu Lei4Jianming Su5Department of Basic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaFumonisins (Fums) are types of mycotoxin that widely contaminante feed material crops, and can trigger potential biological toxicities to humans and various animals. However, the toxicity of Fums on porcine blood vessels has not been fully explored. Fumonisin B<sub>1</sub> (FB<sub>1</sub>) is the main component of Fums. Therefore, the aim of this study was to explore the effects of FB<sub>1</sub> on the oxidative stress and tight junctions of the pig iliac endothelial cells (PIECs) in vitro. The results showed that FB<sub>1</sub> reduced the viability of PIECs, increased the contents of lipid peroxidation product malondialdehyde (MDA), decreased the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thioredoxin reductase (TrxR), and decreased the level of glutathione (GSH). In addition, the barrier functions were destroyed, along with the down-regulations on Claudin 1, Occludin and ZO-1 and the increase of paracellular permeability. Thus, this research indicates that FB<sub>1</sub> facilitates oxidative stress and breaks barrier functions to damage pig iliac endothelium cells.https://www.mdpi.com/2072-6651/11/7/387Fumonisin B<sub>1</sub>pig iliac endothelial cellsoxidative stresstight junction |
| spellingShingle | Qiaoling Yuan Yancheng Jiang Ying Fan Yingfeng Ma Hongyu Lei Jianming Su Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells Toxins Fumonisin B<sub>1</sub> pig iliac endothelial cells oxidative stress tight junction |
| title | Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells |
| title_full | Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells |
| title_fullStr | Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells |
| title_full_unstemmed | Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells |
| title_short | Fumonisin B<sub>1</sub> Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells |
| title_sort | fumonisin b sub 1 sub induces oxidative stress and breaks barrier functions in pig iliac endothelium cells |
| topic | Fumonisin B<sub>1</sub> pig iliac endothelial cells oxidative stress tight junction |
| url | https://www.mdpi.com/2072-6651/11/7/387 |
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