Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum)
Abstract The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity. While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption,...
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| Format: | Article |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19663-x |
| _version_ | 1828218089341190144 |
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| author | Eric Amenyogbe Jun Luo Wei-jie Fu Emmanuel Delwin Abarike Zhong-liang Wang Jian-sheng Huang Christian Larbi Ayisi Gang Chen |
| author_facet | Eric Amenyogbe Jun Luo Wei-jie Fu Emmanuel Delwin Abarike Zhong-liang Wang Jian-sheng Huang Christian Larbi Ayisi Gang Chen |
| author_sort | Eric Amenyogbe |
| collection | DOAJ |
| description | Abstract The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity. While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism. However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits. This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp. RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum). Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet. In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL−1 autochthonous strains for ten weeks and a control diet without supplementation. Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control). Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group. Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture. Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference. On the other hand, glucose was significantly (P < 0.05) higher in the group without supplementation. On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference. Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains. Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters. Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments. The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia. |
| first_indexed | 2024-04-12T15:57:31Z |
| format | Article |
| id | doaj.art-c4709dfe58984b48be6709b26ce778a0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T15:57:31Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-c4709dfe58984b48be6709b26ce778a02022-12-22T03:26:18ZengNature PortfolioScientific Reports2045-23222022-10-0112112110.1038/s41598-022-19663-xEffects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum)Eric Amenyogbe0Jun Luo1Wei-jie Fu2Emmanuel Delwin Abarike3Zhong-liang Wang4Jian-sheng Huang5Christian Larbi Ayisi6Gang Chen7Fishery College, Guangdong Ocean UniversityFishery College, Guangdong Ocean UniversityFishery College, Guangdong Ocean UniversityDepartment of Fisheries and Aquatic Resources Management, University for Development Studies, Nyankpala-CampusFishery College, Guangdong Ocean UniversityFishery College, Guangdong Ocean UniversityDepartment of Water Resources and Sustainable Development, University of Environment and Sustainable Development, PMB SOMANYAFishery College, Guangdong Ocean UniversityAbstract The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity. While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism. However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits. This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp. RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum). Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet. In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL−1 autochthonous strains for ten weeks and a control diet without supplementation. Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control). Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group. Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture. Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference. On the other hand, glucose was significantly (P < 0.05) higher in the group without supplementation. On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference. Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains. Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters. Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments. The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.https://doi.org/10.1038/s41598-022-19663-x |
| spellingShingle | Eric Amenyogbe Jun Luo Wei-jie Fu Emmanuel Delwin Abarike Zhong-liang Wang Jian-sheng Huang Christian Larbi Ayisi Gang Chen Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum) Scientific Reports |
| title | Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum) |
| title_full | Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum) |
| title_fullStr | Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum) |
| title_full_unstemmed | Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum) |
| title_short | Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum) |
| title_sort | effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia rachycentron canadum |
| url | https://doi.org/10.1038/s41598-022-19663-x |
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