Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-06-01
|
| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340916301627 |
| _version_ | 1818311545300451328 |
|---|---|
| author | Mohan C. Manjegowda Paridhi Singhal Gupta Anil M. Limaye |
| author_facet | Mohan C. Manjegowda Paridhi Singhal Gupta Anil M. Limaye |
| author_sort | Mohan C. Manjegowda |
| collection | DOAJ |
| description | Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines. Keywords: GPR30, Antiserum, Affinity purified antibody, Western blotting |
| first_indexed | 2024-12-13T08:03:39Z |
| format | Article |
| id | doaj.art-c47229567961411494587df24ecd4596 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-13T08:03:39Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-c47229567961411494587df24ecd45962022-12-21T23:54:22ZengElsevierData in Brief2352-34092016-06-01710151020Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30Mohan C. Manjegowda0Paridhi Singhal Gupta1Anil M. Limaye2Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, IndiaDepartment of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, IndiaCorresponding author.; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, IndiaRabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines. Keywords: GPR30, Antiserum, Affinity purified antibody, Western blottinghttp://www.sciencedirect.com/science/article/pii/S2352340916301627 |
| spellingShingle | Mohan C. Manjegowda Paridhi Singhal Gupta Anil M. Limaye Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 Data in Brief |
| title | Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 |
| title_full | Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 |
| title_fullStr | Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 |
| title_full_unstemmed | Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 |
| title_short | Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 |
| title_sort | validation data of a rabbit antiserum and affinity purified polyclonal antibody against the n terminus of human gpr30 |
| url | http://www.sciencedirect.com/science/article/pii/S2352340916301627 |
| work_keys_str_mv | AT mohancmanjegowda validationdataofarabbitantiserumandaffinitypurifiedpolyclonalantibodyagainstthenterminusofhumangpr30 AT paridhisinghalgupta validationdataofarabbitantiserumandaffinitypurifiedpolyclonalantibodyagainstthenterminusofhumangpr30 AT anilmlimaye validationdataofarabbitantiserumandaffinitypurifiedpolyclonalantibodyagainstthenterminusofhumangpr30 |