Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures
The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequenti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618302457 |
| _version_ | 1818722960247095296 |
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| author | Kang-Hyun Jeong Si-Young Cho Yong-Deog Hong Jin-Oh Chung Kwang-Sik Kim Soon-Mi Shim |
| author_facet | Kang-Hyun Jeong Si-Young Cho Yong-Deog Hong Jin-Oh Chung Kwang-Sik Kim Soon-Mi Shim |
| author_sort | Kang-Hyun Jeong |
| collection | DOAJ |
| description | The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases. |
| first_indexed | 2024-12-17T21:02:55Z |
| format | Article |
| id | doaj.art-c472cda8d669410dbcd997320956f3b4 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T21:02:55Z |
| publishDate | 2018-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-c472cda8d669410dbcd997320956f3b42022-12-21T21:32:41ZengElsevierJournal of Functional Foods1756-46462018-08-01478390Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell culturesKang-Hyun Jeong0Si-Young Cho1Yong-Deog Hong2Jin-Oh Chung3Kwang-Sik Kim4Soon-Mi Shim5Department of Food Science and Biotechnology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of KoreaBasic Research & Innovation Institute, AmorePacific R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of KoreaVital Beautie Research Institute, AmorePacific R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of KoreaDepartment of Food Science and Biotechnology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea; Vital Beautie Research Institute, AmorePacific R&D Center, Yongin-si, Gyeonggi-do 446-729, Republic of KoreaPediatric Infectious Diseases, Johns Hopkins University, 600 N. Wolfe St, Park 256, Baltimore, MD 21287, USADepartment of Food Science and Biotechnology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea; Corresponding author.The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.http://www.sciencedirect.com/science/article/pii/S1756464618302457GCGCGMetaboliteIn vitro bio-mimic model |
| spellingShingle | Kang-Hyun Jeong Si-Young Cho Yong-Deog Hong Jin-Oh Chung Kwang-Sik Kim Soon-Mi Shim Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures Journal of Functional Foods GCG CG Metabolite In vitro bio-mimic model |
| title | Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures |
| title_full | Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures |
| title_fullStr | Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures |
| title_full_unstemmed | Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures |
| title_short | Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures |
| title_sort | transport of gallocatechin gallate and catechin gallate in high temperature processed green tea extract from gastrointestinal tract to brain by an in vitro bio mimic model system coupled with sequential cell cultures |
| topic | GCG CG Metabolite In vitro bio-mimic model |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618302457 |
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