Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study
Background and Objectives: The incidence of fungal infections in immunocompromised patients, especially by Candida species, has increased in recent years. This study was designed to identify Candida species and determine antifungal susceptibility patterns of 595 yeast strains isolated from various c...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2011-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/111 |
| _version_ | 1831696764538716160 |
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| author | P Badiee A Alborzi |
| author_facet | P Badiee A Alborzi |
| author_sort | P Badiee |
| collection | DOAJ |
| description | Background and Objectives: The incidence of fungal infections in immunocompromised patients, especially by Candida species, has increased in recent years. This study was designed to identify Candida species and determine antifungal susceptibility patterns of 595 yeast strains isolated from various clinical specimens.
Material and Methods: Identification of the isolates were determined by the API 20 C AUX kit and antifungal susceptibilities of the species to fluconazole, amphotericin B, ketoconazole, itraconazole, voriconazole, and caspofungin were determined by the agar-based E-test method.
Results: Candida albicans (48%) was the most frequently isolated species, followed by Candida kruzei (16.1%), Candida glabrata (13.5%), Candida kefyr (7.4%), Candida parapsilosis (4.8%), Candida tropicalis (1.7%) and other species (8.5%). Resistance varies depending on the species and the respective antifungal agents. Comparing the MIC90 for all the strains, the lower MIC90 was observed for caspofungin (0.5 μg/ml). The MIC90 for all Candida species were 64 μg/ml for fluconazole, 0.75 μg/ml for amphotericin B, 4 μg/ml for ketoconazole, 4 μg/ml for itraconazole, and 2 μg/ml for voriconazole.
Conclusions: Species definition and determination of antifungal susceptibility patterns are advised for the proper management and treatment of patients at risk for systemic candidiasis. Resistance to antifungal agents is an alarming sign for the emerging common nosocomial fungal infections. |
| first_indexed | 2024-12-20T13:19:12Z |
| format | Article |
| id | doaj.art-c472ec443ef443cb863f3871608907b2 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-20T13:19:12Z |
| publishDate | 2011-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-c472ec443ef443cb863f3871608907b22022-12-21T19:39:26ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472011-12-0134Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year studyP Badiee0A Alborzi1Prof. Alborzi Microbiology Research Center, Mycology Department, Shiraz University of Medical Sciences, Shiraz, Iran.Prof. Alborzi Microbiology Research Center, Mycology Department, Shiraz University of Medical Sciences, Shiraz, Iran.Background and Objectives: The incidence of fungal infections in immunocompromised patients, especially by Candida species, has increased in recent years. This study was designed to identify Candida species and determine antifungal susceptibility patterns of 595 yeast strains isolated from various clinical specimens. Material and Methods: Identification of the isolates were determined by the API 20 C AUX kit and antifungal susceptibilities of the species to fluconazole, amphotericin B, ketoconazole, itraconazole, voriconazole, and caspofungin were determined by the agar-based E-test method. Results: Candida albicans (48%) was the most frequently isolated species, followed by Candida kruzei (16.1%), Candida glabrata (13.5%), Candida kefyr (7.4%), Candida parapsilosis (4.8%), Candida tropicalis (1.7%) and other species (8.5%). Resistance varies depending on the species and the respective antifungal agents. Comparing the MIC90 for all the strains, the lower MIC90 was observed for caspofungin (0.5 μg/ml). The MIC90 for all Candida species were 64 μg/ml for fluconazole, 0.75 μg/ml for amphotericin B, 4 μg/ml for ketoconazole, 4 μg/ml for itraconazole, and 2 μg/ml for voriconazole. Conclusions: Species definition and determination of antifungal susceptibility patterns are advised for the proper management and treatment of patients at risk for systemic candidiasis. Resistance to antifungal agents is an alarming sign for the emerging common nosocomial fungal infections.https://ijm.tums.ac.ir/index.php/ijm/article/view/111Candidaamphotericin Bitraconazolevoriconazoleantifungal susceptibilityE - test |
| spellingShingle | P Badiee A Alborzi Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study Iranian Journal of Microbiology Candida amphotericin B itraconazole voriconazole antifungal susceptibility E - test |
| title | Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study |
| title_full | Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study |
| title_fullStr | Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study |
| title_full_unstemmed | Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study |
| title_short | Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study |
| title_sort | susceptibility of clinical candida species isolates to antifungal agents by e test southern iran a five year study |
| topic | Candida amphotericin B itraconazole voriconazole antifungal susceptibility E - test |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/111 |
| work_keys_str_mv | AT pbadiee susceptibilityofclinicalcandidaspeciesisolatestoantifungalagentsbyetestsoutherniranafiveyearstudy AT aalborzi susceptibilityofclinicalcandidaspeciesisolatestoantifungalagentsbyetestsoutherniranafiveyearstudy |