Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation

Studies were carried out on the fermentative production of γ-linolenic acid (GLA) using seven strains belonging to Mucorales. An oleaginous fungal strain, isolated from the Western Ghats of Kerala produced GLA at a level of 8 % (by mass), when grown in a complex medium containing glucose as the sole...

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Main Authors: Syed U. Ahmed, Sudheer K. Singh, Ashok Pandey, Sanjit Kanjilal, Rachapudi B. N. Prasad
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2006-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/162002
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author Syed U. Ahmed
Sudheer K. Singh
Ashok Pandey
Sanjit Kanjilal
Rachapudi B. N. Prasad
author_facet Syed U. Ahmed
Sudheer K. Singh
Ashok Pandey
Sanjit Kanjilal
Rachapudi B. N. Prasad
author_sort Syed U. Ahmed
collection DOAJ
description Studies were carried out on the fermentative production of γ-linolenic acid (GLA) using seven strains belonging to Mucorales. An oleaginous fungal strain, isolated from the Western Ghats of Kerala produced GLA at a level of 8 % (by mass), when grown in a complex medium containing glucose as the sole carbon source. Effects of different culture conditions were investigated in shake flasks. Maximum dry biomass and total GLA obtained were 48.4 g/L and 636 mg/L, respectively, in the culture cultivated at 30 °C and 200 rpm for 7 days. Among the organic nitrogen sources investigated, yeast extract, and combination of corn steep liquor and baker’s yeast in 1:1 ratio were useful for enhancing the GLA production and the effects were comparable.
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spelling doaj.art-c4737f0bea454bb9871b4e7091c8420f2023-12-03T06:14:17ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062006-01-01442283287Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged FermentationSyed U. Ahmed0Sudheer K. Singh1Ashok Pandey2Sanjit Kanjilal3Rachapudi B. N. Prasad4Biotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, IndiaBiotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, IndiaBiotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, IndiaOils and Fats Division, Indian Institute of Chemical Technology, Hyderabad 500007, IndiaOils and Fats Division, Indian Institute of Chemical Technology, Hyderabad 500007, IndiaStudies were carried out on the fermentative production of γ-linolenic acid (GLA) using seven strains belonging to Mucorales. An oleaginous fungal strain, isolated from the Western Ghats of Kerala produced GLA at a level of 8 % (by mass), when grown in a complex medium containing glucose as the sole carbon source. Effects of different culture conditions were investigated in shake flasks. Maximum dry biomass and total GLA obtained were 48.4 g/L and 636 mg/L, respectively, in the culture cultivated at 30 °C and 200 rpm for 7 days. Among the organic nitrogen sources investigated, yeast extract, and combination of corn steep liquor and baker’s yeast in 1:1 ratio were useful for enhancing the GLA production and the effects were comparable.http://hrcak.srce.hr/file/162002γ-linolenic acidsubmerged fermentationMucor sp.process parameters
spellingShingle Syed U. Ahmed
Sudheer K. Singh
Ashok Pandey
Sanjit Kanjilal
Rachapudi B. N. Prasad
Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
Food Technology and Biotechnology
γ-linolenic acid
submerged fermentation
Mucor sp.
process parameters
title Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
title_full Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
title_fullStr Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
title_full_unstemmed Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
title_short Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation
title_sort effects of various process parameters on the production of γ linolenic acid in submerged fermentation
topic γ-linolenic acid
submerged fermentation
Mucor sp.
process parameters
url http://hrcak.srce.hr/file/162002
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