Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to dev...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1227 |
| _version_ | 1827658269533929472 |
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| author | Benjamin Tuck Elizabeth Watkin Anthony Somers Maria Forsyth Laura L. Machuca |
| author_facet | Benjamin Tuck Elizabeth Watkin Anthony Somers Maria Forsyth Laura L. Machuca |
| author_sort | Benjamin Tuck |
| collection | DOAJ |
| description | Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to develop and apply environmentally sustainable chemical treatments (biocides); especially in engineered systems associated with the marine environment. Recently, extracellular DNA (eDNA) was implicated as a critical structural polymer in marine biofilms. Additionally, an environmentally sustainable, multi-functional biocide was also introduced to manage corrosion and biofilm formation. To anticipate biofilm tolerance acquisition to chemical treatments and reduce biocide application quantities, the present research investigated eDNA as a target for biofilm dispersal and potential enhancement of biocide function. Results indicate that mature biofilm viability can be reduced by two-fold using reduced concentrations of the biocide alone (1 mM instead of the recommended 10 mM). Importantly, through the incorporation of an eDNA degradation stage, biocide function could be enhanced by a further ~90% (one further log reduction in viability). Biofilm architecture analysis post-treatment revealed that endonuclease targeting of the matrix allowed greater biocide penetration, leading to the observed viability reduction. Biofilm matrix eDNA is a promising target for biofilm dispersal and antimicrobial enhancement in clinical and engineered systems. |
| first_indexed | 2024-03-09T22:59:22Z |
| format | Article |
| id | doaj.art-c4749ec805d3457e852818f72d957743 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:22Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c4749ec805d3457e852818f72d9577432023-11-23T18:05:08ZengMDPI AGMicroorganisms2076-26072022-06-01106122710.3390/microorganisms10061227Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm DisruptionBenjamin Tuck0Elizabeth Watkin1Anthony Somers2Maria Forsyth3Laura L. Machuca4Curtin Corrosion Centre, WA School of Mines: Minerals, Energy and Chemical Engineering, Curtin University, Kent Street, Bentley, WA 6102, AustraliaCurtin Medical School, Curtin University, Kent Street, Bentley, WA 6102, AustraliaInstitute for Frontier Materials, Deakin University, Geelong, VIC 3217, AustraliaInstitute for Frontier Materials, Deakin University, Geelong, VIC 3217, AustraliaCurtin Corrosion Centre, WA School of Mines: Minerals, Energy and Chemical Engineering, Curtin University, Kent Street, Bentley, WA 6102, AustraliaBiofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to develop and apply environmentally sustainable chemical treatments (biocides); especially in engineered systems associated with the marine environment. Recently, extracellular DNA (eDNA) was implicated as a critical structural polymer in marine biofilms. Additionally, an environmentally sustainable, multi-functional biocide was also introduced to manage corrosion and biofilm formation. To anticipate biofilm tolerance acquisition to chemical treatments and reduce biocide application quantities, the present research investigated eDNA as a target for biofilm dispersal and potential enhancement of biocide function. Results indicate that mature biofilm viability can be reduced by two-fold using reduced concentrations of the biocide alone (1 mM instead of the recommended 10 mM). Importantly, through the incorporation of an eDNA degradation stage, biocide function could be enhanced by a further ~90% (one further log reduction in viability). Biofilm architecture analysis post-treatment revealed that endonuclease targeting of the matrix allowed greater biocide penetration, leading to the observed viability reduction. Biofilm matrix eDNA is a promising target for biofilm dispersal and antimicrobial enhancement in clinical and engineered systems.https://www.mdpi.com/2076-2607/10/6/1227extracellular DNAmicrobiologically influenced corrosionbiofilmextracellular polymeric substancesEPScorrosion inhibitor |
| spellingShingle | Benjamin Tuck Elizabeth Watkin Anthony Somers Maria Forsyth Laura L. Machuca Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption Microorganisms extracellular DNA microbiologically influenced corrosion biofilm extracellular polymeric substances EPS corrosion inhibitor |
| title | Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption |
| title_full | Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption |
| title_fullStr | Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption |
| title_full_unstemmed | Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption |
| title_short | Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption |
| title_sort | enhancing biocide efficacy targeting extracellular dna for marine biofilm disruption |
| topic | extracellular DNA microbiologically influenced corrosion biofilm extracellular polymeric substances EPS corrosion inhibitor |
| url | https://www.mdpi.com/2076-2607/10/6/1227 |
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