Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties
Salix integra Thunb., a fast-growing woody plant species, has been used for phytoremediation in recent years. However, little knowledge is available regarding indigenous soil microbial communities associated with the S. integra phytoextraction process. In this study, we used an Illumina MiSeq platfo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00924/full |
| _version_ | 1819227580895592448 |
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| author | Xiaoyun Niu Jian Zhou Xiaona Wang Xiaoyu Su Shaohua Du Yufei Zhu Jinyu Yang Dazhuang Huang |
| author_facet | Xiaoyun Niu Jian Zhou Xiaona Wang Xiaoyu Su Shaohua Du Yufei Zhu Jinyu Yang Dazhuang Huang |
| author_sort | Xiaoyun Niu |
| collection | DOAJ |
| description | Salix integra Thunb., a fast-growing woody plant species, has been used for phytoremediation in recent years. However, little knowledge is available regarding indigenous soil microbial communities associated with the S. integra phytoextraction process. In this study, we used an Illumina MiSeq platform to explore the indigenous microbial composition after planting S. integra at different lead (Pb) contamination levels: no Pb, low Pb treatment (Pb 500 mg kg–1), and high Pb treatment (Pb 1500 mg kg–1). At the same time, the soil properties and their relationship with the bacterial communities were analyzed. The results showed that Pb concentration was highest in the root reaching at 3159.92 ± 138.98 mg kg–1 under the high Pb treatment. Planting S. integra decreased the total Pb concentration by 84.61 and 29.24 mg kg–1, and increased the acid-soluble Pb proportion by 1.0 and 0.75% in the rhizosphere and bulk soil under the low Pb treatment compared with unplanted soil, respectively. However, it occurred only in the rhizosphere soil under the high Pb treatment. The bacterial community structure and microbial metabolism were related to Pb contamination levels and planting of S. integra, while the bacterial diversity was only affected by Pb contamination levels. The dominant microbial species were similar, but their relative abundance shifted in different treatments. Most of the specific bacterial assemblages whose relative abundances were promoted by root activity and/or Pb contamination were suitable for use in plant-microbial combination remediation, especially many genera coming from Proteobacteria. Redundancy analysis (RDA) showed available nitrogen and pH having a significant effect on the bacteria relating to phytoremediation. The results indicated that indigenous bacteria have great potential in the application of combined S. integra-microbe remediation of lead-contaminated soil by adjusting soil properties. |
| first_indexed | 2024-12-23T10:43:39Z |
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| id | doaj.art-c474af1593ef46f89abc27fcb4d03107 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T10:43:39Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c474af1593ef46f89abc27fcb4d031072022-12-21T17:50:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.00924512676Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil PropertiesXiaoyun Niu0Jian Zhou1Xiaona Wang2Xiaoyu Su3Shaohua Du4Yufei Zhu5Jinyu Yang6Dazhuang Huang7College of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, ChinaCollege of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, ChinaCollege of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, ChinaCollege of Forestry, Hebei Agricultural University, Baoding, ChinaCollege of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, ChinaCollege of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, ChinaCollege of Forestry, Hebei Agricultural University, Baoding, ChinaCollege of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, ChinaSalix integra Thunb., a fast-growing woody plant species, has been used for phytoremediation in recent years. However, little knowledge is available regarding indigenous soil microbial communities associated with the S. integra phytoextraction process. In this study, we used an Illumina MiSeq platform to explore the indigenous microbial composition after planting S. integra at different lead (Pb) contamination levels: no Pb, low Pb treatment (Pb 500 mg kg–1), and high Pb treatment (Pb 1500 mg kg–1). At the same time, the soil properties and their relationship with the bacterial communities were analyzed. The results showed that Pb concentration was highest in the root reaching at 3159.92 ± 138.98 mg kg–1 under the high Pb treatment. Planting S. integra decreased the total Pb concentration by 84.61 and 29.24 mg kg–1, and increased the acid-soluble Pb proportion by 1.0 and 0.75% in the rhizosphere and bulk soil under the low Pb treatment compared with unplanted soil, respectively. However, it occurred only in the rhizosphere soil under the high Pb treatment. The bacterial community structure and microbial metabolism were related to Pb contamination levels and planting of S. integra, while the bacterial diversity was only affected by Pb contamination levels. The dominant microbial species were similar, but their relative abundance shifted in different treatments. Most of the specific bacterial assemblages whose relative abundances were promoted by root activity and/or Pb contamination were suitable for use in plant-microbial combination remediation, especially many genera coming from Proteobacteria. Redundancy analysis (RDA) showed available nitrogen and pH having a significant effect on the bacteria relating to phytoremediation. The results indicated that indigenous bacteria have great potential in the application of combined S. integra-microbe remediation of lead-contaminated soil by adjusting soil properties.https://www.frontiersin.org/article/10.3389/fmicb.2020.00924/fullbacterial communitysoil propertiesPb contaminationplant-microbe combined remediationredundancy analysis |
| spellingShingle | Xiaoyun Niu Jian Zhou Xiaona Wang Xiaoyu Su Shaohua Du Yufei Zhu Jinyu Yang Dazhuang Huang Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties Frontiers in Microbiology bacterial community soil properties Pb contamination plant-microbe combined remediation redundancy analysis |
| title | Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties |
| title_full | Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties |
| title_fullStr | Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties |
| title_full_unstemmed | Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties |
| title_short | Indigenous Bacteria Have High Potential for Promoting Salix integra Thunb. Remediation of Lead-Contaminated Soil by Adjusting Soil Properties |
| title_sort | indigenous bacteria have high potential for promoting salix integra thunb remediation of lead contaminated soil by adjusting soil properties |
| topic | bacterial community soil properties Pb contamination plant-microbe combined remediation redundancy analysis |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00924/full |
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