Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni
Campylobacter jejuni is a Gram-negative foodborne bacterium that can cause mild to serious diseases in humans. A variety of stress conditions including exposure to formic acid, a weak organic acid, can cause C. jejuni to form viable but nonculturable cells (VBNC), which was proposed as a potential s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00183/full |
| _version_ | 1828339463405699072 |
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| author | Issmat I Kassem Kshipra eChandrashekhar Gireesh eRajashekara |
| author_facet | Issmat I Kassem Kshipra eChandrashekhar Gireesh eRajashekara |
| author_sort | Issmat I Kassem |
| collection | DOAJ |
| description | Campylobacter jejuni is a Gram-negative foodborne bacterium that can cause mild to serious diseases in humans. A variety of stress conditions including exposure to formic acid, a weak organic acid, can cause C. jejuni to form viable but nonculturable cells (VBNC), which was proposed as a potential survival mechanism. The inability to detect C. jejuni VBNC using standard culturing techniques may increase the risk of exposure to foods contaminated with this pathogen. However, little is known about the cellular mechanisms and triggers governing VBNC formation. Here, we discuss novel mechanisms that potentially affect VBNC formation in C. jejuni and emphasize the impact of formic acid on this process. Specifically, we highlight findings that show that impairing inorganic polyphosphate (poly-P) metabolism reduces the ability of C. jejuni to form VBNC in a medium containing formic acid. We also discuss the potential effect of poly-P and formate metabolism on energy homeostasis and cognate VBNC formation. The relationship between poly-P metabolism and VBNC formation under acid stress has only recently been identified and may represent a breakthrough in understanding this phenomenon and its impact on food safety. |
| first_indexed | 2024-04-13T22:43:05Z |
| format | Article |
| id | doaj.art-c475de023821436390eb78a44f365b87 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T22:43:05Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c475de023821436390eb78a44f365b872022-12-22T02:26:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-07-01410.3389/fmicb.2013.0018352989Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuniIssmat I Kassem0Kshipra eChandrashekhar1Gireesh eRajashekara2The Ohio State UniversityThe Ohio State UniversityThe Ohio State UniversityCampylobacter jejuni is a Gram-negative foodborne bacterium that can cause mild to serious diseases in humans. A variety of stress conditions including exposure to formic acid, a weak organic acid, can cause C. jejuni to form viable but nonculturable cells (VBNC), which was proposed as a potential survival mechanism. The inability to detect C. jejuni VBNC using standard culturing techniques may increase the risk of exposure to foods contaminated with this pathogen. However, little is known about the cellular mechanisms and triggers governing VBNC formation. Here, we discuss novel mechanisms that potentially affect VBNC formation in C. jejuni and emphasize the impact of formic acid on this process. Specifically, we highlight findings that show that impairing inorganic polyphosphate (poly-P) metabolism reduces the ability of C. jejuni to form VBNC in a medium containing formic acid. We also discuss the potential effect of poly-P and formate metabolism on energy homeostasis and cognate VBNC formation. The relationship between poly-P metabolism and VBNC formation under acid stress has only recently been identified and may represent a breakthrough in understanding this phenomenon and its impact on food safety.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00183/fullCampylobacter jejuniSurvivalEnergyFormate dehydrogenaseviable but nonculturable cells (VBNC)inorganic polyphosphate (poly-P) |
| spellingShingle | Issmat I Kassem Kshipra eChandrashekhar Gireesh eRajashekara Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni Frontiers in Microbiology Campylobacter jejuni Survival Energy Formate dehydrogenase viable but nonculturable cells (VBNC) inorganic polyphosphate (poly-P) |
| title | Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni |
| title_full | Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni |
| title_fullStr | Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni |
| title_full_unstemmed | Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni |
| title_short | Of energy and survival incognito: A relationship between viable but non-culturable cells (VBNC) formation and inorganic polyphosphate and formate metabolism in Campylobacter jejuni |
| title_sort | of energy and survival incognito a relationship between viable but non culturable cells vbnc formation and inorganic polyphosphate and formate metabolism in campylobacter jejuni |
| topic | Campylobacter jejuni Survival Energy Formate dehydrogenase viable but nonculturable cells (VBNC) inorganic polyphosphate (poly-P) |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00183/full |
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