Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8
In this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 105 Da. The structural characterization of EPS indicated that the EPS...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.644226/full |
| _version_ | 1818730629768937472 |
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| author | Junrui Wu Danli Yan Yumeng Liu Xue Luo Yang Li Chengxu Cao Mo Li Qi Han Cong Wang Rina Wu Lanwei Zhang |
| author_facet | Junrui Wu Danli Yan Yumeng Liu Xue Luo Yang Li Chengxu Cao Mo Li Qi Han Cong Wang Rina Wu Lanwei Zhang |
| author_sort | Junrui Wu |
| collection | DOAJ |
| description | In this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 105 Da. The structural characterization of EPS indicated that the EPS belonged to the class polysaccharide, mainly composed of glucan and also contained certain mannose residues that were found to be connected by α-1,6 glycosidic bonds. Moreover, the results demonstrated that EPS displayed a significant capacity to scavenge free radical to some extent, and this anti-oxidant potential was found to be concentration dependent. The results further revealed that EPS displayed a significant inhibitory potential on the growth of HepG2 cells by promoting apoptosis and induced cell cycle arrest in G1 and G2 phases. Overall, these results suggested that EPS can be explored as a possible anti-cancer agent. |
| first_indexed | 2024-12-17T23:04:50Z |
| format | Article |
| id | doaj.art-c476c2fd15944f229f2373895d92cd40 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T23:04:50Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c476c2fd15944f229f2373895d92cd402022-12-21T21:29:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-03-011210.3389/fmicb.2021.644226644226Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8Junrui Wu0Danli Yan1Yumeng Liu2Xue Luo3Yang Li4Chengxu Cao5Mo Li6Qi Han7Cong Wang8Rina Wu9Lanwei Zhang10College of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao, ChinaIn this study, a novel exopolysaccharide (EPS) was extracted from Leuconostoc mesenteroides Shen Nong’s (SN)-8 which can be obtained from Dajiang. After the purification step, EPS-8-2 was obtained with molecular weights of 1.46 × 105 Da. The structural characterization of EPS indicated that the EPS belonged to the class polysaccharide, mainly composed of glucan and also contained certain mannose residues that were found to be connected by α-1,6 glycosidic bonds. Moreover, the results demonstrated that EPS displayed a significant capacity to scavenge free radical to some extent, and this anti-oxidant potential was found to be concentration dependent. The results further revealed that EPS displayed a significant inhibitory potential on the growth of HepG2 cells by promoting apoptosis and induced cell cycle arrest in G1 and G2 phases. Overall, these results suggested that EPS can be explored as a possible anti-cancer agent.https://www.frontiersin.org/articles/10.3389/fmicb.2021.644226/fullLeuconostoc mesenteroidesexopolysaccharide (EPS)characterizationantitumor activityapoptosisproliferation |
| spellingShingle | Junrui Wu Danli Yan Yumeng Liu Xue Luo Yang Li Chengxu Cao Mo Li Qi Han Cong Wang Rina Wu Lanwei Zhang Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 Frontiers in Microbiology Leuconostoc mesenteroides exopolysaccharide (EPS) characterization antitumor activity apoptosis proliferation |
| title | Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 |
| title_full | Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 |
| title_fullStr | Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 |
| title_full_unstemmed | Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 |
| title_short | Purification, Structural Characteristics, and Biological Activities of Exopolysaccharide Isolated From Leuconostoc mesenteroides SN-8 |
| title_sort | purification structural characteristics and biological activities of exopolysaccharide isolated from leuconostoc mesenteroides sn 8 |
| topic | Leuconostoc mesenteroides exopolysaccharide (EPS) characterization antitumor activity apoptosis proliferation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.644226/full |
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