Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results
Alahmady Hamad Alsmman,1 Gamal Radwan,1 Mortada Ahmed Abozaid,1 Usama Ali Mohammed,1 Nesreen Gamal Abd Elhaleim2 1Department of Ophthalmology, 2Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt Purpose: The aim of this study was to detect the clinical and histologi...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-03-01
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| Series: | Clinical Ophthalmology |
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| Online Access: | https://www.dovepress.com/preoperative-subconjunctival-combined-injection-of-bevacizumab-and-mit-peer-reviewed-article-OPTH |
| _version_ | 1818845334522036224 |
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| author | Alsmman AH Radwan G Abozaid MA Mohammed UA Abd Elhaliem NG |
| author_facet | Alsmman AH Radwan G Abozaid MA Mohammed UA Abd Elhaliem NG |
| author_sort | Alsmman AH |
| collection | DOAJ |
| description | Alahmady Hamad Alsmman,1 Gamal Radwan,1 Mortada Ahmed Abozaid,1 Usama Ali Mohammed,1 Nesreen Gamal Abd Elhaleim2 1Department of Ophthalmology, 2Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt Purpose: The aim of this study was to detect the clinical and histological effects of preoperative subconjunctival injection of both bevacizumab and mitomycin C (MMC) 1 month before the surgical excision of primary pterygium using a bare sclera technique. Patients and methods: A total of 20 patients with primary pterygium underwent subconjunctival combined injection of 0.1 mL of MMC (0.1 mg/mL) and 0.1 mL of bevacizumab (1.25 mg/0.1 mL) 1 month before bare sclera excision of the pterygium. The excised pterygium tissues were examined histologically and immunohistologically by CD31 staining, and the patients were followed up clinically for at least 2 years. The excised pterygia of two patients without preoperative injection were used for histological comparison. Results: Clinically, there were no intraoperative or postoperative complications. No recurrence was noted during the follow-up period. Histologically, the previously injected pterygia showed a decreased number of epithelial cells and stromal fibroblasts. The latter were rounded or oval and swollen rather than spindle shaped, and some were degenerating or apoptotic. Collagen and elastic fibers were degenerated, distorted, and decreased in density, while blood capillaries were obliterated. There was a significant decrease in CD31-positive cells in previously injected pterygia. Conclusion: Preoperative subpterygium combined injection of bevacizumab and MMC is safe and effective in reducing the postoperative recurrence of primary pterygium. Histological and immunohistological changes in the form of decreased fibrovascular activity and degeneration of the extracellular matrix and nerve axons were noted. Keywords: subconjunctival bevacizumab, subconjunctival mitomycin C, histological changes, primary pterygium, CD31 |
| first_indexed | 2024-12-19T05:28:01Z |
| format | Article |
| id | doaj.art-c47a06f8c101479ca218918fa60a1633 |
| institution | Directory Open Access Journal |
| issn | 1177-5483 |
| language | English |
| last_indexed | 2024-12-19T05:28:01Z |
| publishDate | 2017-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical Ophthalmology |
| spelling | doaj.art-c47a06f8c101479ca218918fa60a16332022-12-21T20:34:20ZengDove Medical PressClinical Ophthalmology1177-54832017-03-01Volume 1149350131768Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological resultsAlsmman AHRadwan GAbozaid MAMohammed UAAbd Elhaliem NGAlahmady Hamad Alsmman,1 Gamal Radwan,1 Mortada Ahmed Abozaid,1 Usama Ali Mohammed,1 Nesreen Gamal Abd Elhaleim2 1Department of Ophthalmology, 2Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt Purpose: The aim of this study was to detect the clinical and histological effects of preoperative subconjunctival injection of both bevacizumab and mitomycin C (MMC) 1 month before the surgical excision of primary pterygium using a bare sclera technique. Patients and methods: A total of 20 patients with primary pterygium underwent subconjunctival combined injection of 0.1 mL of MMC (0.1 mg/mL) and 0.1 mL of bevacizumab (1.25 mg/0.1 mL) 1 month before bare sclera excision of the pterygium. The excised pterygium tissues were examined histologically and immunohistologically by CD31 staining, and the patients were followed up clinically for at least 2 years. The excised pterygia of two patients without preoperative injection were used for histological comparison. Results: Clinically, there were no intraoperative or postoperative complications. No recurrence was noted during the follow-up period. Histologically, the previously injected pterygia showed a decreased number of epithelial cells and stromal fibroblasts. The latter were rounded or oval and swollen rather than spindle shaped, and some were degenerating or apoptotic. Collagen and elastic fibers were degenerated, distorted, and decreased in density, while blood capillaries were obliterated. There was a significant decrease in CD31-positive cells in previously injected pterygia. Conclusion: Preoperative subpterygium combined injection of bevacizumab and MMC is safe and effective in reducing the postoperative recurrence of primary pterygium. Histological and immunohistological changes in the form of decreased fibrovascular activity and degeneration of the extracellular matrix and nerve axons were noted. Keywords: subconjunctival bevacizumab, subconjunctival mitomycin C, histological changes, primary pterygium, CD31https://www.dovepress.com/preoperative-subconjunctival-combined-injection-of-bevacizumab-and-mit-peer-reviewed-article-OPTHsubconjunctival bevacizumabsubconjunctival mitomycin Chistolog-ical changesprimary pterygiumCD31. |
| spellingShingle | Alsmman AH Radwan G Abozaid MA Mohammed UA Abd Elhaliem NG Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results Clinical Ophthalmology subconjunctival bevacizumab subconjunctival mitomycin C histolog-ical changes primary pterygium CD31. |
| title | Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results |
| title_full | Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results |
| title_fullStr | Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results |
| title_full_unstemmed | Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results |
| title_short | Preoperative subconjunctival combined injection of bevacizumab and mitomycin C before the surgical excision of primary pterygium: clinical and histological results |
| title_sort | preoperative subconjunctival combined injection of bevacizumab and mitomycin c before the surgical excision of primary pterygium clinical and histological results |
| topic | subconjunctival bevacizumab subconjunctival mitomycin C histolog-ical changes primary pterygium CD31. |
| url | https://www.dovepress.com/preoperative-subconjunctival-combined-injection-of-bevacizumab-and-mit-peer-reviewed-article-OPTH |
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