Electricity from lignocellulosic substrates by thermophilic Geobacillus species
Abstract Given our vast lignocellulosic biomass reserves and the difficulty in bioprocessing them without expensive pretreatment and fuel separation steps, the conversion of lignocellulosic biomass directly into electricity would be beneficial. Here we report the previously unexplored capabilities o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-72866-y |
| _version_ | 1819148641874477056 |
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| author | Namita Shrestha Abhilash Kumar Tripathi Tanvi Govil Rajesh Kumar Sani Meltem Urgun-Demirtas Venkateswaran Kasthuri Venkataramana Gadhamshetty |
| author_facet | Namita Shrestha Abhilash Kumar Tripathi Tanvi Govil Rajesh Kumar Sani Meltem Urgun-Demirtas Venkateswaran Kasthuri Venkataramana Gadhamshetty |
| author_sort | Namita Shrestha |
| collection | DOAJ |
| description | Abstract Given our vast lignocellulosic biomass reserves and the difficulty in bioprocessing them without expensive pretreatment and fuel separation steps, the conversion of lignocellulosic biomass directly into electricity would be beneficial. Here we report the previously unexplored capabilities of thermophilic Geobacillus sp. strain WSUCF1 to generate electricity directly from such complex substrates in microbial fuel cells. This process obviates the need for exogenous enzymes and redox mediator supplements. Cyclic voltammetry and chromatography studies revealed the electrochemical signatures of riboflavin molecules that reflect mediated electron transfer capabilities of strain WSUCF1. Proteomics and genomics analysis corroborated that WSUCF1 biofilms uses type-II NADH dehydrogenase and demethylmenaquinone methyltransferase to transfer the electrons to conducting anode via the redox active pheromone lipoproteins localized at the cell membrane. |
| first_indexed | 2024-12-22T13:48:57Z |
| format | Article |
| id | doaj.art-c47d24ebd4324053ba551b5829fc9e2f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T13:48:57Z |
| publishDate | 2020-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c47d24ebd4324053ba551b5829fc9e2f2022-12-21T18:23:44ZengNature PortfolioScientific Reports2045-23222020-10-011011910.1038/s41598-020-72866-yElectricity from lignocellulosic substrates by thermophilic Geobacillus speciesNamita Shrestha0Abhilash Kumar Tripathi1Tanvi Govil2Rajesh Kumar Sani3Meltem Urgun-Demirtas4Venkateswaran Kasthuri5Venkataramana Gadhamshetty6Civil and Environmental Engineering, South Dakota School of Mines and TechnologyDepartment of Biological and Chemical Engineering, South Dakota School of Mines and TechnologyDepartment of Biological and Chemical Engineering, South Dakota School of Mines and TechnologyDepartment of Biological and Chemical Engineering, South Dakota School of Mines and TechnologyEnergy Global Security Division, Argonne National LaboratoryBiotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of TechnologyCivil and Environmental Engineering, South Dakota School of Mines and TechnologyAbstract Given our vast lignocellulosic biomass reserves and the difficulty in bioprocessing them without expensive pretreatment and fuel separation steps, the conversion of lignocellulosic biomass directly into electricity would be beneficial. Here we report the previously unexplored capabilities of thermophilic Geobacillus sp. strain WSUCF1 to generate electricity directly from such complex substrates in microbial fuel cells. This process obviates the need for exogenous enzymes and redox mediator supplements. Cyclic voltammetry and chromatography studies revealed the electrochemical signatures of riboflavin molecules that reflect mediated electron transfer capabilities of strain WSUCF1. Proteomics and genomics analysis corroborated that WSUCF1 biofilms uses type-II NADH dehydrogenase and demethylmenaquinone methyltransferase to transfer the electrons to conducting anode via the redox active pheromone lipoproteins localized at the cell membrane.https://doi.org/10.1038/s41598-020-72866-y |
| spellingShingle | Namita Shrestha Abhilash Kumar Tripathi Tanvi Govil Rajesh Kumar Sani Meltem Urgun-Demirtas Venkateswaran Kasthuri Venkataramana Gadhamshetty Electricity from lignocellulosic substrates by thermophilic Geobacillus species Scientific Reports |
| title | Electricity from lignocellulosic substrates by thermophilic Geobacillus species |
| title_full | Electricity from lignocellulosic substrates by thermophilic Geobacillus species |
| title_fullStr | Electricity from lignocellulosic substrates by thermophilic Geobacillus species |
| title_full_unstemmed | Electricity from lignocellulosic substrates by thermophilic Geobacillus species |
| title_short | Electricity from lignocellulosic substrates by thermophilic Geobacillus species |
| title_sort | electricity from lignocellulosic substrates by thermophilic geobacillus species |
| url | https://doi.org/10.1038/s41598-020-72866-y |
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