Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes
In this work, we report an analytical procedure to investigate the lipid compositions of sheep and goat milk. This approach is based on an ion mobility–high-resolution mass spectrometric method to facilitate the identification of complex lipid species and their regiochemistry. A common triacylglycer...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/6/3535 |
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| author | Cristina Manis Paola Scano Viviana Garau Margherita Addis Ignazio Ibba Pierluigi Caboni |
| author_facet | Cristina Manis Paola Scano Viviana Garau Margherita Addis Ignazio Ibba Pierluigi Caboni |
| author_sort | Cristina Manis |
| collection | DOAJ |
| description | In this work, we report an analytical procedure to investigate the lipid compositions of sheep and goat milk. This approach is based on an ion mobility–high-resolution mass spectrometric method to facilitate the identification of complex lipid species and their regiochemistry. A common triacylglycerol profile was observed for sheep and goat milk samples, while a higher abundance of medium-chain fatty acids was observed at the sn-2 position for sheep milk. Furthermore, differences can be also observed in the levels of saturated and unsaturated fatty acids at the sn-2 position. In terms of lipid classes, goat milk showed higher levels of triacylglycerols, phosphatidylinositols and ether-linked phosphatidylethanolamines, while sheep milk showed higher levels of free fatty acids, lysophosphatidylethanolamines, lysophosphocholines and non-hydroxy fatty acid-dihydrosphingosine ceramides when compared with goat milk. |
| first_indexed | 2024-03-11T06:59:55Z |
| format | Article |
| id | doaj.art-c47dde24fe71416fa3ecd3e72912f41e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T06:59:55Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-c47dde24fe71416fa3ecd3e72912f41e2023-11-17T09:23:05ZengMDPI AGApplied Sciences2076-34172023-03-01136353510.3390/app13063535Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk LipidomesCristina Manis0Paola Scano1Viviana Garau2Margherita Addis3Ignazio Ibba4Pierluigi Caboni5Department of Life and Environmental Sciences, University of Cagliari, 09042 Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09042 Cagliari, ItalyScuola di Specializzazione in Farmacia Ospedaliera, Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23a, 07100 Sassari, ItalyAgris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040 Olmedo, ItalyAssociazione Regionale Allevatori, Laboratorio Analisi del Latte, Loc. Palloni, Nuraxinieddu, 09170 Oristano, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09042 Cagliari, ItalyIn this work, we report an analytical procedure to investigate the lipid compositions of sheep and goat milk. This approach is based on an ion mobility–high-resolution mass spectrometric method to facilitate the identification of complex lipid species and their regiochemistry. A common triacylglycerol profile was observed for sheep and goat milk samples, while a higher abundance of medium-chain fatty acids was observed at the sn-2 position for sheep milk. Furthermore, differences can be also observed in the levels of saturated and unsaturated fatty acids at the sn-2 position. In terms of lipid classes, goat milk showed higher levels of triacylglycerols, phosphatidylinositols and ether-linked phosphatidylethanolamines, while sheep milk showed higher levels of free fatty acids, lysophosphatidylethanolamines, lysophosphocholines and non-hydroxy fatty acid-dihydrosphingosine ceramides when compared with goat milk.https://www.mdpi.com/2076-3417/13/6/3535lipidomicstriacylglycerolslipid regiochemistryIM-QTOF-MS analysis |
| spellingShingle | Cristina Manis Paola Scano Viviana Garau Margherita Addis Ignazio Ibba Pierluigi Caboni Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes Applied Sciences lipidomics triacylglycerols lipid regiochemistry IM-QTOF-MS analysis |
| title | Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes |
| title_full | Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes |
| title_fullStr | Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes |
| title_full_unstemmed | Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes |
| title_short | Ion Mobility–Mass Spectrometry Approach for the Comparison of Sheep and Goat Milk Lipidomes |
| title_sort | ion mobility mass spectrometry approach for the comparison of sheep and goat milk lipidomes |
| topic | lipidomics triacylglycerols lipid regiochemistry IM-QTOF-MS analysis |
| url | https://www.mdpi.com/2076-3417/13/6/3535 |
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