The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children.
EV71 is one of major etiologic causes of hand-foot-mouth disease (HFMD) and leads to severe neurological complications in young children and infants. Recently inactivated EV71 vaccines have been developed by five manufactures and clinically show good safety and immunogenicity. However, the cross-neu...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3834186?pdf=render |
| _version_ | 1818313834956324864 |
|---|---|
| author | Qunying Mao Tong Cheng Fengcai Zhu Jingxin Li Yiping Wang Yanping Li Fan Gao Lisheng Yang Xin Yao Jie Shao Ningshao Xia Zhenglun Liang Junzhi Wang |
| author_facet | Qunying Mao Tong Cheng Fengcai Zhu Jingxin Li Yiping Wang Yanping Li Fan Gao Lisheng Yang Xin Yao Jie Shao Ningshao Xia Zhenglun Liang Junzhi Wang |
| author_sort | Qunying Mao |
| collection | DOAJ |
| description | EV71 is one of major etiologic causes of hand-foot-mouth disease (HFMD) and leads to severe neurological complications in young children and infants. Recently inactivated EV71 vaccines have been developed by five manufactures and clinically show good safety and immunogenicity. However, the cross-neutralizing activity of these vaccines remains unclear, and is of particular interest because RNA recombination is seen more frequently in EV71 epidemics.In this post-hoc study, sera from a subset of 119 infants and children in two clinical trials of EV71 subgenotype C4 vaccines (ClinicalTrials.gov Identifier: NCT01313715 and NCT01273246), were detected for neutralizing antibody (NTAb) titres with sera from infected patients as controls. Cytopathogenic effect method was employed to test NTAb against EV71 subgenotype B4, B5, C2, C4 and C5, which were prominent epidemic strains worldwide over the past decade. To validate the accuracy of the results, ELISpot assay was employed in parallel to detect NTAb in all the post-vaccine sera. After two-dose vaccination, 49 out of 53 participants in initially seronegative group and 52 out of 53 participants in initially seropositive group showed less than 4-fold differences in NTAb titers against five EV71 strains, whereas corresponding values among sera from pediatric patients recovering from EV71-induced HFMD and subclinically infected participants were 8/8 and 41/43, respectively. The geometric mean titers of participants against five subgenotypes EV71 all grew significantly after vaccinations, irrespective of the baseline NTAb titer. The relative fold increase in antibody titers (NTAb-FI) against B4, B5, C2, and C5 displayed a positive correlation to the NTAb-FI against C4.The results demonstrated broad cross-neutralizing activity induced by two C4 EV71 vaccines in healthy Chinese infants and children. However, the degree of induced cross-protective immunity, and the potential escape evolution for EV71 still need to be monitored and researched in future for these new vaccines. |
| first_indexed | 2024-12-13T08:40:03Z |
| format | Article |
| id | doaj.art-c47eaebab199449fb8647be28f9894c2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T08:40:03Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c47eaebab199449fb8647be28f9894c22022-12-21T23:53:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7959910.1371/journal.pone.0079599The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children.Qunying MaoTong ChengFengcai ZhuJingxin LiYiping WangYanping LiFan GaoLisheng YangXin YaoJie ShaoNingshao XiaZhenglun LiangJunzhi WangEV71 is one of major etiologic causes of hand-foot-mouth disease (HFMD) and leads to severe neurological complications in young children and infants. Recently inactivated EV71 vaccines have been developed by five manufactures and clinically show good safety and immunogenicity. However, the cross-neutralizing activity of these vaccines remains unclear, and is of particular interest because RNA recombination is seen more frequently in EV71 epidemics.In this post-hoc study, sera from a subset of 119 infants and children in two clinical trials of EV71 subgenotype C4 vaccines (ClinicalTrials.gov Identifier: NCT01313715 and NCT01273246), were detected for neutralizing antibody (NTAb) titres with sera from infected patients as controls. Cytopathogenic effect method was employed to test NTAb against EV71 subgenotype B4, B5, C2, C4 and C5, which were prominent epidemic strains worldwide over the past decade. To validate the accuracy of the results, ELISpot assay was employed in parallel to detect NTAb in all the post-vaccine sera. After two-dose vaccination, 49 out of 53 participants in initially seronegative group and 52 out of 53 participants in initially seropositive group showed less than 4-fold differences in NTAb titers against five EV71 strains, whereas corresponding values among sera from pediatric patients recovering from EV71-induced HFMD and subclinically infected participants were 8/8 and 41/43, respectively. The geometric mean titers of participants against five subgenotypes EV71 all grew significantly after vaccinations, irrespective of the baseline NTAb titer. The relative fold increase in antibody titers (NTAb-FI) against B4, B5, C2, and C5 displayed a positive correlation to the NTAb-FI against C4.The results demonstrated broad cross-neutralizing activity induced by two C4 EV71 vaccines in healthy Chinese infants and children. However, the degree of induced cross-protective immunity, and the potential escape evolution for EV71 still need to be monitored and researched in future for these new vaccines.http://europepmc.org/articles/PMC3834186?pdf=render |
| spellingShingle | Qunying Mao Tong Cheng Fengcai Zhu Jingxin Li Yiping Wang Yanping Li Fan Gao Lisheng Yang Xin Yao Jie Shao Ningshao Xia Zhenglun Liang Junzhi Wang The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children. PLoS ONE |
| title | The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children. |
| title_full | The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children. |
| title_fullStr | The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children. |
| title_full_unstemmed | The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children. |
| title_short | The cross-neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children. |
| title_sort | cross neutralizing activity of enterovirus 71 subgenotype c4 vaccines in healthy chinese infants and children |
| url | http://europepmc.org/articles/PMC3834186?pdf=render |
| work_keys_str_mv | AT qunyingmao thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT tongcheng thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT fengcaizhu thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT jingxinli thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT yipingwang thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT yanpingli thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT fangao thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT lishengyang thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT xinyao thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT jieshao thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT ningshaoxia thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT zhenglunliang thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT junzhiwang thecrossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT qunyingmao crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT tongcheng crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT fengcaizhu crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT jingxinli crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT yipingwang crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT yanpingli crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT fangao crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT lishengyang crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT xinyao crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT jieshao crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT ningshaoxia crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT zhenglunliang crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren AT junzhiwang crossneutralizingactivityofenterovirus71subgenotypec4vaccinesinhealthychineseinfantsandchildren |