Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process
Aquaporins (AQPs) are essential membrane proteins involved in seed maturation and germination, stomata movement, photosynthesis, and regulation of plant flowering processes. Pitaya flowers are open at night and wither at daybreak, which shows an obvious circadian rhythm. In this study, a comprehensi...
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/14/7689 |
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| author | Xiaoying Ye Yongshun Gao Canbin Chen Fangfang Xie Qingzhu Hua Zhike Zhang Rong Zhang Jietang Zhao Guibing Hu Yonghua Qin |
| author_facet | Xiaoying Ye Yongshun Gao Canbin Chen Fangfang Xie Qingzhu Hua Zhike Zhang Rong Zhang Jietang Zhao Guibing Hu Yonghua Qin |
| author_sort | Xiaoying Ye |
| collection | DOAJ |
| description | Aquaporins (AQPs) are essential membrane proteins involved in seed maturation and germination, stomata movement, photosynthesis, and regulation of plant flowering processes. Pitaya flowers are open at night and wither at daybreak, which shows an obvious circadian rhythm. In this study, a comprehensive genome-wide analysis of <i>AQPs</i> in <i>Hylocereus undantus</i> was conducted to screen key genes associated with flowering processes. A total of 33 <i>HuAQP</i> genes were identified from the <i>H. undantus</i> genome. The 33 <i>HuAQPs</i> were grouped into four subfamilies: 10 PIPs, 13 TIPs, 8 NIPs, and 2 SIPs, which were distributed on 9 out of 11 pitaya chromosomes (Chr) (except for Chr7 and Chr10). Results from expression profiles showed that <i>HuNIP6;1</i> may be involved in pitaya’s floral opening. <i>HuNIP6;1</i> was localized exclusively in the cell membrane. Overexpression of <i>HuNIP6;1</i> in <i>Arabidopsis thaliana</i> significantly promoted early flowering through regulating negative flowering regulators of <i>MJM30</i>, <i>COL9</i>, and <i>PRR5</i>, suggesting that <i>HuNIP6;1</i> plays key roles in regulating flowering time. The present study provides the first genome-wide analysis of the <i>AQP</i> gene family in pitaya and valuable information for utilization of <i>HuAQPs</i>. |
| first_indexed | 2024-03-10T09:36:38Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c486dfb436c74a869199ebe6fbd4199b2023-11-22T04:02:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214768910.3390/ijms22147689Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering ProcessXiaoying Ye0Yongshun Gao1Canbin Chen2Fangfang Xie3Qingzhu Hua4Zhike Zhang5Rong Zhang6Jietang Zhao7Guibing Hu8Yonghua Qin9Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaAquaporins (AQPs) are essential membrane proteins involved in seed maturation and germination, stomata movement, photosynthesis, and regulation of plant flowering processes. Pitaya flowers are open at night and wither at daybreak, which shows an obvious circadian rhythm. In this study, a comprehensive genome-wide analysis of <i>AQPs</i> in <i>Hylocereus undantus</i> was conducted to screen key genes associated with flowering processes. A total of 33 <i>HuAQP</i> genes were identified from the <i>H. undantus</i> genome. The 33 <i>HuAQPs</i> were grouped into four subfamilies: 10 PIPs, 13 TIPs, 8 NIPs, and 2 SIPs, which were distributed on 9 out of 11 pitaya chromosomes (Chr) (except for Chr7 and Chr10). Results from expression profiles showed that <i>HuNIP6;1</i> may be involved in pitaya’s floral opening. <i>HuNIP6;1</i> was localized exclusively in the cell membrane. Overexpression of <i>HuNIP6;1</i> in <i>Arabidopsis thaliana</i> significantly promoted early flowering through regulating negative flowering regulators of <i>MJM30</i>, <i>COL9</i>, and <i>PRR5</i>, suggesting that <i>HuNIP6;1</i> plays key roles in regulating flowering time. The present study provides the first genome-wide analysis of the <i>AQP</i> gene family in pitaya and valuable information for utilization of <i>HuAQPs</i>.https://www.mdpi.com/1422-0067/22/14/7689pitayaaquaporingenome-wide identification<i>HuNIP61</i>early flowering |
| spellingShingle | Xiaoying Ye Yongshun Gao Canbin Chen Fangfang Xie Qingzhu Hua Zhike Zhang Rong Zhang Jietang Zhao Guibing Hu Yonghua Qin Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process International Journal of Molecular Sciences pitaya aquaporin genome-wide identification <i>HuNIP6 1</i> early flowering |
| title | Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process |
| title_full | Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process |
| title_fullStr | Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process |
| title_full_unstemmed | Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process |
| title_short | Genome-Wide Identification of <i>Aquaporin</i> Gene Family in Pitaya Reveals an <i>HuNIP6;1</i> Involved in Flowering Process |
| title_sort | genome wide identification of i aquaporin i gene family in pitaya reveals an i hunip6 1 i involved in flowering process |
| topic | pitaya aquaporin genome-wide identification <i>HuNIP6 1</i> early flowering |
| url | https://www.mdpi.com/1422-0067/22/14/7689 |
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