Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry
Coccidiosis is a disease caused by intracellular protozoan parasites of the genus <i>Eimeria</i> that affect the intestinal tract of poultry. However, strain resistance and drug residue in the carcass have drawn the attention of the productive sector. The nanotechnology can improve the b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/2/392 |
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| author | Lana Flávia Baron Francisco Noé da Fonseca Shaiana Salete Maciag Franciana Aparecida Volpato Bellaver Adriana Mércia Guaratini Ibeli Marcos Antônio Zanella Mores Gabryelle Furtado de Almeida Silvia Stanisçuaski Guterres Ana Paula Almeida Bastos Karina Paese |
| author_facet | Lana Flávia Baron Francisco Noé da Fonseca Shaiana Salete Maciag Franciana Aparecida Volpato Bellaver Adriana Mércia Guaratini Ibeli Marcos Antônio Zanella Mores Gabryelle Furtado de Almeida Silvia Stanisçuaski Guterres Ana Paula Almeida Bastos Karina Paese |
| author_sort | Lana Flávia Baron |
| collection | DOAJ |
| description | Coccidiosis is a disease caused by intracellular protozoan parasites of the genus <i>Eimeria</i> that affect the intestinal tract of poultry. However, strain resistance and drug residue in the carcass have drawn the attention of the productive sector. The nanotechnology can improve the biological effect of drugs, reducing of administered doses and toxic effects. Due to this, toltrazuril-load polymeric nanoparticles based on Eudragit<sup>®</sup> S100 (NCt) or poly-ε-caprolactone (LNCt) were developed to prevent coccidiosis in broilers. Nanoformulations were produced and showed homogeneous particle diameter distribution in the nanometer range (<i>z</i>-average and D (4.3) < 200 nm), negative zeta potential (<−8.93 mV), drug content ~100%, and encapsulation efficiency >90%. Cell viability assays using avian fibroblasts showed that LNCt presented no relevant toxicity up to 72 h. LNCt was then prophylactically administrated to chicken followed by challenge with <i>Eimeria</i> oocysts. The evaluation of the small intestine and cecum showed that the treatment with LNCt (3.5 mg/kg/day) in drinking water reduced the lesion scores and oocysts excretion, similar to the reference medicine containing toltrazuril (Baycox<sup>®</sup>, 7 mg/kg/day). The current study shows the potential protective use of nanoencapsulating anticoccidial drugs as a promising approach for the control of coccidiosis in poultry. |
| first_indexed | 2024-03-09T21:14:19Z |
| format | Article |
| id | doaj.art-c48709faed9e46638d4e1b1aff1f7fca |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:14:19Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-c48709faed9e46638d4e1b1aff1f7fca2023-11-23T21:38:33ZengMDPI AGPharmaceutics1999-49232022-02-0114239210.3390/pharmaceutics14020392Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in PoultryLana Flávia Baron0Francisco Noé da Fonseca1Shaiana Salete Maciag2Franciana Aparecida Volpato Bellaver3Adriana Mércia Guaratini Ibeli4Marcos Antônio Zanella Mores5Gabryelle Furtado de Almeida6Silvia Stanisçuaski Guterres7Ana Paula Almeida Bastos8Karina Paese9Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, BrazilEmbrapa Suínos e Aves, BR 153 Km 110 s/n, Concórdia 89715-899, SC, BrazilCampus CEDETEG, Universidade Estadual do Centro-Oeste do Paraná, Al. Elio Antonio Dalla Vecchia, 838, Guarapuava 85040-167, PR, BrazilInstituto Federal Catarinense, Campus Concórdia, Rd. SC 283 s/n, Concórdia 89703-720, SC, BrazilEmbrapa Suínos e Aves, BR 153 Km 110 s/n, Concórdia 89715-899, SC, BrazilEmbrapa Suínos e Aves, BR 153 Km 110 s/n, Concórdia 89715-899, SC, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, BrazilEmbrapa Suínos e Aves, BR 153 Km 110 s/n, Concórdia 89715-899, SC, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, BrazilCoccidiosis is a disease caused by intracellular protozoan parasites of the genus <i>Eimeria</i> that affect the intestinal tract of poultry. However, strain resistance and drug residue in the carcass have drawn the attention of the productive sector. The nanotechnology can improve the biological effect of drugs, reducing of administered doses and toxic effects. Due to this, toltrazuril-load polymeric nanoparticles based on Eudragit<sup>®</sup> S100 (NCt) or poly-ε-caprolactone (LNCt) were developed to prevent coccidiosis in broilers. Nanoformulations were produced and showed homogeneous particle diameter distribution in the nanometer range (<i>z</i>-average and D (4.3) < 200 nm), negative zeta potential (<−8.93 mV), drug content ~100%, and encapsulation efficiency >90%. Cell viability assays using avian fibroblasts showed that LNCt presented no relevant toxicity up to 72 h. LNCt was then prophylactically administrated to chicken followed by challenge with <i>Eimeria</i> oocysts. The evaluation of the small intestine and cecum showed that the treatment with LNCt (3.5 mg/kg/day) in drinking water reduced the lesion scores and oocysts excretion, similar to the reference medicine containing toltrazuril (Baycox<sup>®</sup>, 7 mg/kg/day). The current study shows the potential protective use of nanoencapsulating anticoccidial drugs as a promising approach for the control of coccidiosis in poultry.https://www.mdpi.com/1999-4923/14/2/392Avian coccidiosis<i>Eimeria</i> spp.toltrazurilpolymeric nanocapsulesEudragit<sup>®</sup> S100poly-ε-caprolactone |
| spellingShingle | Lana Flávia Baron Francisco Noé da Fonseca Shaiana Salete Maciag Franciana Aparecida Volpato Bellaver Adriana Mércia Guaratini Ibeli Marcos Antônio Zanella Mores Gabryelle Furtado de Almeida Silvia Stanisçuaski Guterres Ana Paula Almeida Bastos Karina Paese Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry Pharmaceutics Avian coccidiosis <i>Eimeria</i> spp. toltrazuril polymeric nanocapsules Eudragit<sup>®</sup> S100 poly-ε-caprolactone |
| title | Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry |
| title_full | Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry |
| title_fullStr | Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry |
| title_full_unstemmed | Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry |
| title_short | Toltrazuril-Loaded Polymeric Nanocapsules as a Promising Approach for the Preventive Control of Coccidiosis in Poultry |
| title_sort | toltrazuril loaded polymeric nanocapsules as a promising approach for the preventive control of coccidiosis in poultry |
| topic | Avian coccidiosis <i>Eimeria</i> spp. toltrazuril polymeric nanocapsules Eudragit<sup>®</sup> S100 poly-ε-caprolactone |
| url | https://www.mdpi.com/1999-4923/14/2/392 |
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