Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i>
<i>Pyrrhoderma noxium</i> is a plant fungal pathogen that induces the disease of brown root rot in a large variety of tree species. It is currently infecting many of the amenity trees within Brisbane City of Queensland, Australia. Steering away from harmful chemical fungicides, biologica...
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MDPI AG
2023-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1373 |
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| author | Cherrihan Adra Trong D. Tran Keith Foster Russell Tomlin D. İpek Kurtböke |
| author_facet | Cherrihan Adra Trong D. Tran Keith Foster Russell Tomlin D. İpek Kurtböke |
| author_sort | Cherrihan Adra |
| collection | DOAJ |
| description | <i>Pyrrhoderma noxium</i> is a plant fungal pathogen that induces the disease of brown root rot in a large variety of tree species. It is currently infecting many of the amenity trees within Brisbane City of Queensland, Australia. Steering away from harmful chemical fungicides, biological control agents offer environmentally friendly alternatives. Streptomycetes are known for their production of novel bioactive secondary metabolites with biocontrol potential, particularly, streptomycete symbionts isolated from unique ecological niches. In this study, 37 termite gut-associated actinomycete isolates were identified using molecular methods and screened against <i>P. noxium</i>. A majority of the isolates belonged to the genus <i>Streptomyces</i>, and 15 isolates exhibited strong antifungal activity with up to 98.5% mycelial inhibition of the fungal pathogen. MS/MS molecular networking analysis of the isolates’ fermentation extracts revealed several chemical classes with polyketides being among the most abundant. Most of the metabolites, however, did not have matches to the GNPS database, indicating potential novel antifungal compounds in the active extracts obtained from the isolates. Pathway enrichment and overrepresentation analyses revealed pathways relating to polyketide antibiotic production, among other antibiotic pathways, further confirming the biosynthetic potential of the termite gut-associated streptomycetes with biocontrol potential against <i>P. noxium</i>. |
| first_indexed | 2024-03-10T23:06:48Z |
| format | Article |
| id | doaj.art-c48a684a3c9845a1b44f080603a9696c |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:48Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c48a684a3c9845a1b44f080603a9696c2023-11-19T09:16:52ZengMDPI AGAntibiotics2079-63822023-08-01129137310.3390/antibiotics12091373Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i>Cherrihan Adra0Trong D. Tran1Keith Foster2Russell Tomlin3D. İpek Kurtböke4School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore BC, QLD 4558, AustraliaSchool of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore BC, QLD 4558, AustraliaBrisbane City Council, Program, Planning and Integration, Brisbane Square, Level 10, 266 George Street, Brisbane, QLD 4000, AustraliaBrisbane City Council, Program, Planning and Integration, Brisbane Square, Level 10, 266 George Street, Brisbane, QLD 4000, AustraliaSchool of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore BC, QLD 4558, Australia<i>Pyrrhoderma noxium</i> is a plant fungal pathogen that induces the disease of brown root rot in a large variety of tree species. It is currently infecting many of the amenity trees within Brisbane City of Queensland, Australia. Steering away from harmful chemical fungicides, biological control agents offer environmentally friendly alternatives. Streptomycetes are known for their production of novel bioactive secondary metabolites with biocontrol potential, particularly, streptomycete symbionts isolated from unique ecological niches. In this study, 37 termite gut-associated actinomycete isolates were identified using molecular methods and screened against <i>P. noxium</i>. A majority of the isolates belonged to the genus <i>Streptomyces</i>, and 15 isolates exhibited strong antifungal activity with up to 98.5% mycelial inhibition of the fungal pathogen. MS/MS molecular networking analysis of the isolates’ fermentation extracts revealed several chemical classes with polyketides being among the most abundant. Most of the metabolites, however, did not have matches to the GNPS database, indicating potential novel antifungal compounds in the active extracts obtained from the isolates. Pathway enrichment and overrepresentation analyses revealed pathways relating to polyketide antibiotic production, among other antibiotic pathways, further confirming the biosynthetic potential of the termite gut-associated streptomycetes with biocontrol potential against <i>P. noxium</i>.https://www.mdpi.com/2079-6382/12/9/1373<i>Streptomyces</i>streptomycetes<i>Pyrrhoderma noxium</i>termitestermite gut symbiosisantifungal compounds |
| spellingShingle | Cherrihan Adra Trong D. Tran Keith Foster Russell Tomlin D. İpek Kurtböke Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i> Antibiotics <i>Streptomyces</i> streptomycetes <i>Pyrrhoderma noxium</i> termites termite gut symbiosis antifungal compounds |
| title | Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i> |
| title_full | Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i> |
| title_fullStr | Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i> |
| title_full_unstemmed | Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i> |
| title_short | Untargeted MS-Based Metabolomic Analysis of Termite Gut-Associated Streptomycetes with Antifungal Activity against <i>Pyrrhoderma noxium</i> |
| title_sort | untargeted ms based metabolomic analysis of termite gut associated streptomycetes with antifungal activity against i pyrrhoderma noxium i |
| topic | <i>Streptomyces</i> streptomycetes <i>Pyrrhoderma noxium</i> termites termite gut symbiosis antifungal compounds |
| url | https://www.mdpi.com/2079-6382/12/9/1373 |
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