Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China
<i>Hydnobolites</i> is an ectomycorrhizal fungal genus with hypogeous ascomata in the family <i>Pezizaceae</i> (<i>Pezizales</i>). Molecular analyses of <i>Hydnobolites</i> using both single (ITS) and concatenated gene datasets (ITS-nLSU) showed a tota...
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/12/1302 |
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| author | Shan-Ping Wan Lan-Lan Huang Meng-Jin Cui Cheng-Jin Yu Wei Liu Rui Wang Xiao-Fei Shi Fu-Qiang Yu |
| author_facet | Shan-Ping Wan Lan-Lan Huang Meng-Jin Cui Cheng-Jin Yu Wei Liu Rui Wang Xiao-Fei Shi Fu-Qiang Yu |
| author_sort | Shan-Ping Wan |
| collection | DOAJ |
| description | <i>Hydnobolites</i> is an ectomycorrhizal fungal genus with hypogeous ascomata in the family <i>Pezizaceae</i> (<i>Pezizales</i>). Molecular analyses of <i>Hydnobolites</i> using both single (ITS) and concatenated gene datasets (ITS-nLSU) showed a total of 223 sequences, including 92 newly gained sequences from Chinese specimens. Phylogenetic results based on these two datasets revealed seven distinct phylogenetic clades. Among them, the ITS phylogenetic tree confirmed the presence of at least 42 phylogenetic species in <i>Hydnobolites</i>. Combined the morphological observations with molecular analyses, five new species of <i>Hydnobolites translucidus</i> sp. nov., <i>H. subrufus</i> sp. nov., <i>H. lini</i> sp. nov., <i>H. sichuanensis</i> sp. nov. and <i>H. tenuiperidius</i> sp. nov., and one new record species of <i>H. cerebriformis</i> Tul., were illustrated from Southwest China. Macro- and micro-morphological analyses of ascomata revealed a few, but diagnostic differences between the <i>H. cerebriformis</i> complex, while the similarities of the ITS sequences ranged from 94.4 to 97.2% resulting in well-supported clades. |
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| language | English |
| last_indexed | 2024-03-09T16:14:15Z |
| publishDate | 2022-12-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-c48b2b80dc524da2a21a5a2ae06d91d72023-11-24T16:00:09ZengMDPI AGJournal of Fungi2309-608X2022-12-01812130210.3390/jof8121302Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from ChinaShan-Ping Wan0Lan-Lan Huang1Meng-Jin Cui2Cheng-Jin Yu3Wei Liu4Rui Wang5Xiao-Fei Shi6Fu-Qiang Yu7College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming 650201, ChinaThe Germplasm Bank of Wild Species, Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, ChinaThe Germplasm Bank of Wild Species, Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, China<i>Hydnobolites</i> is an ectomycorrhizal fungal genus with hypogeous ascomata in the family <i>Pezizaceae</i> (<i>Pezizales</i>). Molecular analyses of <i>Hydnobolites</i> using both single (ITS) and concatenated gene datasets (ITS-nLSU) showed a total of 223 sequences, including 92 newly gained sequences from Chinese specimens. Phylogenetic results based on these two datasets revealed seven distinct phylogenetic clades. Among them, the ITS phylogenetic tree confirmed the presence of at least 42 phylogenetic species in <i>Hydnobolites</i>. Combined the morphological observations with molecular analyses, five new species of <i>Hydnobolites translucidus</i> sp. nov., <i>H. subrufus</i> sp. nov., <i>H. lini</i> sp. nov., <i>H. sichuanensis</i> sp. nov. and <i>H. tenuiperidius</i> sp. nov., and one new record species of <i>H. cerebriformis</i> Tul., were illustrated from Southwest China. Macro- and micro-morphological analyses of ascomata revealed a few, but diagnostic differences between the <i>H. cerebriformis</i> complex, while the similarities of the ITS sequences ranged from 94.4 to 97.2% resulting in well-supported clades.https://www.mdpi.com/2309-608X/8/12/1302Ascomycotahypogeous fungi<i>Pezizaceae</i>phylogenytaxonomy |
| spellingShingle | Shan-Ping Wan Lan-Lan Huang Meng-Jin Cui Cheng-Jin Yu Wei Liu Rui Wang Xiao-Fei Shi Fu-Qiang Yu Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China Journal of Fungi Ascomycota hypogeous fungi <i>Pezizaceae</i> phylogeny taxonomy |
| title | Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China |
| title_full | Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China |
| title_fullStr | Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China |
| title_full_unstemmed | Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China |
| title_short | Phylogenetic Analyses of <i>Hydnobolites</i> and New Species from China |
| title_sort | phylogenetic analyses of i hydnobolites i and new species from china |
| topic | Ascomycota hypogeous fungi <i>Pezizaceae</i> phylogeny taxonomy |
| url | https://www.mdpi.com/2309-608X/8/12/1302 |
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