Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions
Background: Liquid biopsy is a rapidly growing field, for it may provide a minimally invasive way to acquire pathological data for personalized medicine. This study developed a systemic strategy to discover an effective salivary biomarker for early detection of patients with head-neck squamous carci...
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MDPI AG
2021-08-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/8/1411 |
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| author | Ann-Joy Cheng Guo-Rung You Che-Jui Lee Ya-Ching Lu Shang-Ju Tang Yi-Fang Huang Yu-Chen Huang Li-Yu Lee Kang-Hsing Fan Yen-Chao Chen Shiang-Fu Huang Joseph Tung-Chieh Chang |
| author_facet | Ann-Joy Cheng Guo-Rung You Che-Jui Lee Ya-Ching Lu Shang-Ju Tang Yi-Fang Huang Yu-Chen Huang Li-Yu Lee Kang-Hsing Fan Yen-Chao Chen Shiang-Fu Huang Joseph Tung-Chieh Chang |
| author_sort | Ann-Joy Cheng |
| collection | DOAJ |
| description | Background: Liquid biopsy is a rapidly growing field, for it may provide a minimally invasive way to acquire pathological data for personalized medicine. This study developed a systemic strategy to discover an effective salivary biomarker for early detection of patients with head-neck squamous carcinoma (HNSC) and oral precancer lesion (OPC). Methods: A total of 10 miRNAs were examined in parallel with multiple independent cohorts. These included a training set of salivary samples from HNSC patients, the TCGA-HNSC and GSE31277 cohorts to differentiate miRNAs between tumor and normal tissues, and groups of salivary samples from healthy individuals, patients with HNSC and OPC. Results: The combined results from the salivary training set and the TCGA-HNSC cohort showed that four miRNAs (miR-148b, miR-155, miR-196b, and miR-31) consistently increased in HNSC patients. Further integration with the GSE31277 cohort, two miRNAs (miR-31 and miR-196b) maintained at high significances. Further assessment showed that salivary miR-196b was a prominent diagnostic biomarker, as it remarkably discriminated between healthy individuals and patients with HNSC (<i>p</i> < 0.0001, AUC = 0.767, OR = 5.64) or OPC (<i>p</i> < 0.0001, AUC = 0.979, OR = 459). Conclusion: Salivary miR-196b could be an excellent biomarker for diagnosing OPC and early detection of HNSC. This molecule may be used for early screening high-risk groups of HNSC. |
| first_indexed | 2024-03-10T08:53:56Z |
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| id | doaj.art-c48b873e7a1241cdbabfd70291e44de1 |
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| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T08:53:56Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-c48b873e7a1241cdbabfd70291e44de12023-11-22T07:20:07ZengMDPI AGDiagnostics2075-44182021-08-01118141110.3390/diagnostics11081411Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer LesionsAnn-Joy Cheng0Guo-Rung You1Che-Jui Lee2Ya-Ching Lu3Shang-Ju Tang4Yi-Fang Huang5Yu-Chen Huang6Li-Yu Lee7Kang-Hsing Fan8Yen-Chao Chen9Shiang-Fu Huang10Joseph Tung-Chieh Chang11Department of Radiation Oncology and Proton Therapy Center, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of General Dentistry, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Oral and Maxillofacial Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Pathology, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Radiation Oncology and Proton Therapy Center, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Radiation Oncology, Keelung Chang Gung Memorial Hospital, Keelung 20401, TaiwanDepartment of Radiation Oncology and Proton Therapy Center, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Radiation Oncology and Proton Therapy Center, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, TaiwanBackground: Liquid biopsy is a rapidly growing field, for it may provide a minimally invasive way to acquire pathological data for personalized medicine. This study developed a systemic strategy to discover an effective salivary biomarker for early detection of patients with head-neck squamous carcinoma (HNSC) and oral precancer lesion (OPC). Methods: A total of 10 miRNAs were examined in parallel with multiple independent cohorts. These included a training set of salivary samples from HNSC patients, the TCGA-HNSC and GSE31277 cohorts to differentiate miRNAs between tumor and normal tissues, and groups of salivary samples from healthy individuals, patients with HNSC and OPC. Results: The combined results from the salivary training set and the TCGA-HNSC cohort showed that four miRNAs (miR-148b, miR-155, miR-196b, and miR-31) consistently increased in HNSC patients. Further integration with the GSE31277 cohort, two miRNAs (miR-31 and miR-196b) maintained at high significances. Further assessment showed that salivary miR-196b was a prominent diagnostic biomarker, as it remarkably discriminated between healthy individuals and patients with HNSC (<i>p</i> < 0.0001, AUC = 0.767, OR = 5.64) or OPC (<i>p</i> < 0.0001, AUC = 0.979, OR = 459). Conclusion: Salivary miR-196b could be an excellent biomarker for diagnosing OPC and early detection of HNSC. This molecule may be used for early screening high-risk groups of HNSC.https://www.mdpi.com/2075-4418/11/8/1411miR-196bsalivary biomarkerhead-neck canceroral precancer lesiondiagnosisearly detection |
| spellingShingle | Ann-Joy Cheng Guo-Rung You Che-Jui Lee Ya-Ching Lu Shang-Ju Tang Yi-Fang Huang Yu-Chen Huang Li-Yu Lee Kang-Hsing Fan Yen-Chao Chen Shiang-Fu Huang Joseph Tung-Chieh Chang Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions Diagnostics miR-196b salivary biomarker head-neck cancer oral precancer lesion diagnosis early detection |
| title | Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions |
| title_full | Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions |
| title_fullStr | Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions |
| title_full_unstemmed | Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions |
| title_short | Systemic Investigation Identifying Salivary miR-196b as a Promising Biomarker for Early Detection of Head-Neck Cancer and Oral Precancer Lesions |
| title_sort | systemic investigation identifying salivary mir 196b as a promising biomarker for early detection of head neck cancer and oral precancer lesions |
| topic | miR-196b salivary biomarker head-neck cancer oral precancer lesion diagnosis early detection |
| url | https://www.mdpi.com/2075-4418/11/8/1411 |
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