Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability
The endocannabinoid system possesses neuromodulatory functions by influencing the release of various neurotransmitters, including γ-aminobutyric acid (GABA) and glutamate. A functional interaction between endocannabinoids and the serotonergic system has already been suggested. Previously, we showed...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-09-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnbeh.2015.00235/full |
| _version_ | 1819209137705189376 |
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| author | Beat eLutz Martin eHäring Vanessa eEnk Alejandro eAparisi Rey Sebastian eLoch Inigo eRuiz De Azua Krisztina eMonory Tillmann eWeber Dusan eBartsch |
| author_facet | Beat eLutz Martin eHäring Vanessa eEnk Alejandro eAparisi Rey Sebastian eLoch Inigo eRuiz De Azua Krisztina eMonory Tillmann eWeber Dusan eBartsch |
| author_sort | Beat eLutz |
| collection | DOAJ |
| description | The endocannabinoid system possesses neuromodulatory functions by influencing the release of various neurotransmitters, including γ-aminobutyric acid (GABA) and glutamate. A functional interaction between endocannabinoids and the serotonergic system has already been suggested. Previously, we showed that cannabinoid type 1 (CB1) receptor mRNA and protein are localized in serotonergic neurons of the raphe nuclei, implying that the endocannabinoid system can modulate serotonergic functions. In order to substantiate the physiological role of the CB1 receptor in serotonergic neurons of the raphe nuclei, we generated serotonergic (5-HT) neuron-specific CB1 receptor-deficient mice, using the Cre/loxP system with a tamoxifen-inducible Cre recombinase under the control of the regulatory sequences of the tryptophan hydroxylase 2 gene (TPH2-CreERT2), thus, restricting the recombination to 5-HT neurons of the central nervous system. Applying several different behavioral paradigms, we revealed that mice lacking the CB1 receptor in serotonergic neurons are more anxious and less sociable than control littermates. Thus, we were able to show that functional CB1 receptor signaling in central serotonergic neurons modulates distinct behaviors in mice. |
| first_indexed | 2024-12-23T05:50:30Z |
| format | Article |
| id | doaj.art-c48efe2446d64f95aaf35891c4e1d35c |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-12-23T05:50:30Z |
| publishDate | 2015-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-c48efe2446d64f95aaf35891c4e1d35c2022-12-21T17:57:59ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532015-09-01910.3389/fnbeh.2015.00235155948Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociabilityBeat eLutz0Martin eHäring1Vanessa eEnk2Alejandro eAparisi Rey3Sebastian eLoch4Inigo eRuiz De Azua5Krisztina eMonory6Tillmann eWeber7Dusan eBartsch8University Medical Center of the Johannes Gutenberg University MainzUniversity Medical Center of the Johannes Gutenberg University MainzUniversity Medical Center of the Johannes Gutenberg University MainzUniversity Medical Center of the Johannes Gutenberg University MainzUniversity Medical Center of the Johannes Gutenberg University MainzUniversity Medical Center of the Johannes Gutenberg University MainzUniversity Medical Center of the Johannes Gutenberg University MainzCentral Institute of Mental HealthCentral Institute of Mental HealthThe endocannabinoid system possesses neuromodulatory functions by influencing the release of various neurotransmitters, including γ-aminobutyric acid (GABA) and glutamate. A functional interaction between endocannabinoids and the serotonergic system has already been suggested. Previously, we showed that cannabinoid type 1 (CB1) receptor mRNA and protein are localized in serotonergic neurons of the raphe nuclei, implying that the endocannabinoid system can modulate serotonergic functions. In order to substantiate the physiological role of the CB1 receptor in serotonergic neurons of the raphe nuclei, we generated serotonergic (5-HT) neuron-specific CB1 receptor-deficient mice, using the Cre/loxP system with a tamoxifen-inducible Cre recombinase under the control of the regulatory sequences of the tryptophan hydroxylase 2 gene (TPH2-CreERT2), thus, restricting the recombination to 5-HT neurons of the central nervous system. Applying several different behavioral paradigms, we revealed that mice lacking the CB1 receptor in serotonergic neurons are more anxious and less sociable than control littermates. Thus, we were able to show that functional CB1 receptor signaling in central serotonergic neurons modulates distinct behaviors in mice.http://journal.frontiersin.org/Journal/10.3389/fnbeh.2015.00235/fullAnxietyRaphe NucleiSerotoninsociabilityCB1 receptor |
| spellingShingle | Beat eLutz Martin eHäring Vanessa eEnk Alejandro eAparisi Rey Sebastian eLoch Inigo eRuiz De Azua Krisztina eMonory Tillmann eWeber Dusan eBartsch Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability Frontiers in Behavioral Neuroscience Anxiety Raphe Nuclei Serotonin sociability CB1 receptor |
| title | Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability |
| title_full | Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability |
| title_fullStr | Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability |
| title_full_unstemmed | Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability |
| title_short | Cannabinoid type-1 receptor signaling in central serotonergic neurons regulates anxiety-like behavior and sociability |
| title_sort | cannabinoid type 1 receptor signaling in central serotonergic neurons regulates anxiety like behavior and sociability |
| topic | Anxiety Raphe Nuclei Serotonin sociability CB1 receptor |
| url | http://journal.frontiersin.org/Journal/10.3389/fnbeh.2015.00235/full |
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