Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain
Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience. Recent advances in tissue clearing and light sheet microsco...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Neuroanatomy |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fnana.2019.00002/full |
| _version_ | 1811236213448769536 |
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| author | Daniel Normen Düring Daniel Normen Düring Daniel Normen Düring Mariana Diales Rocha Falk Dittrich Manfred Gahr Richard Hans Robert Hahnloser Richard Hans Robert Hahnloser |
| author_facet | Daniel Normen Düring Daniel Normen Düring Daniel Normen Düring Mariana Diales Rocha Falk Dittrich Manfred Gahr Richard Hans Robert Hahnloser Richard Hans Robert Hahnloser |
| author_sort | Daniel Normen Düring |
| collection | DOAJ |
| description | Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience. Recent advances in tissue clearing and light sheet microscopy have re-addressed this challenge and blossomed into a plethora of protocols with diverse advantages and disadvantages. While refractive index matching achieves near perfect transparency and allows for imaging at large depths, the resolution of cleared brains is usually limited to the micrometer range. Moreover, the often long and harsh tissue clearing protocols hinder preservation of native fluorescence and antigenicity. Here we image large expanded brain volumes of zebra finch brain tissue in commercially available light sheet microscopes. Our expansion light sheet microscopy (ExLSM) approach presents a viable alternative to many clearing and imaging methods because it improves on tissue processing times, fluorophore compatibility, and image resolution. |
| first_indexed | 2024-04-12T12:05:01Z |
| format | Article |
| id | doaj.art-c48f0775357a4a14b723d5e1c39bdb34 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-12T12:05:01Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-c48f0775357a4a14b723d5e1c39bdb342022-12-22T03:33:44ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292019-01-011310.3389/fnana.2019.00002428416Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird BrainDaniel Normen Düring0Daniel Normen Düring1Daniel Normen Düring2Mariana Diales Rocha3Falk Dittrich4Manfred Gahr5Richard Hans Robert Hahnloser6Richard Hans Robert Hahnloser7Institute of Neuroinformatics, University of Zürich/ETH Zürich, Zurich, SwitzerlandNeuroscience Center Zurich (ZNZ), Zurich, SwitzerlandDepartment of Behavioral Neurobiology, Max Planck Institute for Ornithology, Seewiesen, GermanyDepartment of Behavioral Neurobiology, Max Planck Institute for Ornithology, Seewiesen, GermanyDepartment of Behavioral Neurobiology, Max Planck Institute for Ornithology, Seewiesen, GermanyDepartment of Behavioral Neurobiology, Max Planck Institute for Ornithology, Seewiesen, GermanyInstitute of Neuroinformatics, University of Zürich/ETH Zürich, Zurich, SwitzerlandNeuroscience Center Zurich (ZNZ), Zurich, SwitzerlandExpansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience. Recent advances in tissue clearing and light sheet microscopy have re-addressed this challenge and blossomed into a plethora of protocols with diverse advantages and disadvantages. While refractive index matching achieves near perfect transparency and allows for imaging at large depths, the resolution of cleared brains is usually limited to the micrometer range. Moreover, the often long and harsh tissue clearing protocols hinder preservation of native fluorescence and antigenicity. Here we image large expanded brain volumes of zebra finch brain tissue in commercially available light sheet microscopes. Our expansion light sheet microscopy (ExLSM) approach presents a viable alternative to many clearing and imaging methods because it improves on tissue processing times, fluorophore compatibility, and image resolution.https://www.frontiersin.org/article/10.3389/fnana.2019.00002/fullexpansion microscopytissue clearingsongbirdspine morphologylight sheet microscopylarge volume imaging |
| spellingShingle | Daniel Normen Düring Daniel Normen Düring Daniel Normen Düring Mariana Diales Rocha Falk Dittrich Manfred Gahr Richard Hans Robert Hahnloser Richard Hans Robert Hahnloser Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain Frontiers in Neuroanatomy expansion microscopy tissue clearing songbird spine morphology light sheet microscopy large volume imaging |
| title | Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain |
| title_full | Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain |
| title_fullStr | Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain |
| title_full_unstemmed | Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain |
| title_short | Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain |
| title_sort | expansion light sheet microscopy resolves subcellular structures in large portions of the songbird brain |
| topic | expansion microscopy tissue clearing songbird spine morphology light sheet microscopy large volume imaging |
| url | https://www.frontiersin.org/article/10.3389/fnana.2019.00002/full |
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