Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation
Recent studies have shown that the metal adaptation of Actinobacteria offers a rich source of metal inducible environmentally relevant bio-compounds and molecules. These interact through biosorption towards the unique cell walls or via metal chelating activity of metallophors with trace elements, he...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340920306338 |
| _version_ | 1818212094830444544 |
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| author | Ringo Schwabe Christoph Helmut Rudi Senges Julia Elisabeth Bandow Thomas Heine Henry Lehmann Oliver Wiche Michael Schlömann Gloria Levicán Dirk Tischler |
| author_facet | Ringo Schwabe Christoph Helmut Rudi Senges Julia Elisabeth Bandow Thomas Heine Henry Lehmann Oliver Wiche Michael Schlömann Gloria Levicán Dirk Tischler |
| author_sort | Ringo Schwabe |
| collection | DOAJ |
| description | Recent studies have shown that the metal adaptation of Actinobacteria offers a rich source of metal inducible environmentally relevant bio-compounds and molecules. These interact through biosorption towards the unique cell walls or via metal chelating activity of metallophors with trace elements, heavy metals and even with lanthanides to overcome limitations and toxic concentrations. Herein, the purpose is to investigate the adaptation potential of Gordonia rubripertincta CWB2 in dependence of the rare earths and to determine if we can utilize promising metallophore metal affinities for metal separation from aquatic solutions. For details on data interpretation and applicability of siderophores we refer to the related article entitled “Cultivation dependent formation of siderophores by Gordonia rubripertincta CWB2” [1].The respective workflow comprises a metal adaptation method to demonstrate effects on bacterial growth, pH, metallophore production, and metabolic change. All this was evaluated by LC-MS/MS and effects on biosorption of rare earths was verified by ICP-MS. Furthermore, we were able to carry out batch metal adsorption and desorption studies of metallophores entrapped in inorganic polymers of tetramethoxysilane (TMOS) to determine metal chelating capacities and selective enrichment effects from model solutions. The adaptation potential of strain CWB2 at increased erbium and manganese concentrations was verified by increased chelating activity on agar plates, in liquid assays and demonstrated by the successful enrichment of erbium by metallophore-functionalized TMOS-polymers from an aquatic model solution. Furthermore, the number of detected compounds in dependency of rare earths differ in spectral counts and diversity compared to the wild type. Finally, the biosorption of rare earths for the selected adaptation was increased significantly up to 2-fold compared to the wild-type. Overall a holistic approach to metal stress was utilised, integrating a bacterial erbium adaptation, metal chelating, biosorption of lanthanides and immobilization as well as enrichment of metals using metallophore functionalized inorganic TMOS polymers for separation of metals from aquatic model solutions. |
| first_indexed | 2024-12-12T05:42:56Z |
| format | Article |
| id | doaj.art-c4911868d9e84124b2de1f2819617a9a |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-12T05:42:56Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-c4911868d9e84124b2de1f2819617a9a2022-12-22T00:35:52ZengElsevierData in Brief2352-34092020-08-0131105739Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptationRingo Schwabe0Christoph Helmut Rudi Senges1Julia Elisabeth Bandow2Thomas Heine3Henry Lehmann4Oliver Wiche5Michael Schlömann6Gloria Levicán7Dirk Tischler8Institute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany; Institute of Biosciences, Biology and Ecology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany; Corresponding authorsApplied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, GermanyApplied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, GermanyInstitute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, GermanyInstitute of Informatic, TU Bergakademie Freiberg, Bernhard-von-Cotta Straße 2, 09599 Freiberg, GermanyInstitute of Biosciences, Biology and Ecology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, GermanyInstitute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, GermanyLaboratorio de Microbiología Básica y Aplicada, Facultad de Química y Biología, Universidad de Santiago, ChileInstitute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany; Microbial Biotechnology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany; Corresponding authorsRecent studies have shown that the metal adaptation of Actinobacteria offers a rich source of metal inducible environmentally relevant bio-compounds and molecules. These interact through biosorption towards the unique cell walls or via metal chelating activity of metallophors with trace elements, heavy metals and even with lanthanides to overcome limitations and toxic concentrations. Herein, the purpose is to investigate the adaptation potential of Gordonia rubripertincta CWB2 in dependence of the rare earths and to determine if we can utilize promising metallophore metal affinities for metal separation from aquatic solutions. For details on data interpretation and applicability of siderophores we refer to the related article entitled “Cultivation dependent formation of siderophores by Gordonia rubripertincta CWB2” [1].The respective workflow comprises a metal adaptation method to demonstrate effects on bacterial growth, pH, metallophore production, and metabolic change. All this was evaluated by LC-MS/MS and effects on biosorption of rare earths was verified by ICP-MS. Furthermore, we were able to carry out batch metal adsorption and desorption studies of metallophores entrapped in inorganic polymers of tetramethoxysilane (TMOS) to determine metal chelating capacities and selective enrichment effects from model solutions. The adaptation potential of strain CWB2 at increased erbium and manganese concentrations was verified by increased chelating activity on agar plates, in liquid assays and demonstrated by the successful enrichment of erbium by metallophore-functionalized TMOS-polymers from an aquatic model solution. Furthermore, the number of detected compounds in dependency of rare earths differ in spectral counts and diversity compared to the wild type. Finally, the biosorption of rare earths for the selected adaptation was increased significantly up to 2-fold compared to the wild-type. Overall a holistic approach to metal stress was utilised, integrating a bacterial erbium adaptation, metal chelating, biosorption of lanthanides and immobilization as well as enrichment of metals using metallophore functionalized inorganic TMOS polymers for separation of metals from aquatic model solutions.http://www.sciencedirect.com/science/article/pii/S2352340920306338MetallophoreHeavy metalsRare earth elementsDissolutionChelating agentACTINOBACTERIA |
| spellingShingle | Ringo Schwabe Christoph Helmut Rudi Senges Julia Elisabeth Bandow Thomas Heine Henry Lehmann Oliver Wiche Michael Schlömann Gloria Levicán Dirk Tischler Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation Data in Brief Metallophore Heavy metals Rare earth elements Dissolution Chelating agent ACTINOBACTERIA |
| title | Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation |
| title_full | Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation |
| title_fullStr | Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation |
| title_full_unstemmed | Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation |
| title_short | Data on metal-chelating, -immobilisation and biosorption properties by Gordonia rubripertincta CWB2 in dependency on rare earth adaptation |
| title_sort | data on metal chelating immobilisation and biosorption properties by gordonia rubripertincta cwb2 in dependency on rare earth adaptation |
| topic | Metallophore Heavy metals Rare earth elements Dissolution Chelating agent ACTINOBACTERIA |
| url | http://www.sciencedirect.com/science/article/pii/S2352340920306338 |
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