Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging
Diastolic dysfunction of the heart and decreased compliance of the vasculature and lungs (i.e., increased organ tissue stiffness) are known features of obesity and the metabolic syndrome. Similarly, cardiac diastolic dysfunction is associated with aging. Elevation of the enzyme transglutaminase 2 (T...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-09-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.560019/full |
| _version_ | 1819019372976406528 |
|---|---|
| author | Krishna C. Penumatsa Ines Falcão-Pires Sara Leite Adelino Leite-Moreira Chinmayee D. Bhedi Sabina Nasirova Jing Ma Jing Ma Roy L. Sutliff Roy L. Sutliff Barry L. Fanburg |
| author_facet | Krishna C. Penumatsa Ines Falcão-Pires Sara Leite Adelino Leite-Moreira Chinmayee D. Bhedi Sabina Nasirova Jing Ma Jing Ma Roy L. Sutliff Roy L. Sutliff Barry L. Fanburg |
| author_sort | Krishna C. Penumatsa |
| collection | DOAJ |
| description | Diastolic dysfunction of the heart and decreased compliance of the vasculature and lungs (i.e., increased organ tissue stiffness) are known features of obesity and the metabolic syndrome. Similarly, cardiac diastolic dysfunction is associated with aging. Elevation of the enzyme transglutaminase 2 (TG2) leads to protein cross-linking and enhanced collagen synthesis and participates as a candidate pathway for development of tissue stiffness. With these observations in mind we hypothesized that TG2 may be elevated in tissues of a rat model of obesity/metabolic syndrome (the ZSF 1 rat) and a mouse model of aging, i.e., the senescent SAMP8 mouse. In the experiments reported here, TG2 expression and activity were found for the first time to be spontaneously elevated in organs from both the ZSF1 rat and the SAMP8 mouse. These observations are consistent with a hypothesis that a TG2-related pathway may participate in the known tissue stiffness associated with cardiac diastolic dysfunction in these two rodent models. The potential TG2 pathway needs better correlation with physiologic dysfunction and may eventually provide novel therapeutic insights to improve tissue compliance. |
| first_indexed | 2024-12-21T03:34:17Z |
| format | Article |
| id | doaj.art-c493d84ab73b45c896c1a9b34ef67050 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T03:34:17Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-c493d84ab73b45c896c1a9b34ef670502022-12-21T19:17:23ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-09-011110.3389/fphys.2020.560019560019Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and AgingKrishna C. Penumatsa0Ines Falcão-Pires1Sara Leite2Adelino Leite-Moreira3Chinmayee D. Bhedi4Sabina Nasirova5Jing Ma6Jing Ma7Roy L. Sutliff8Roy L. Sutliff9Barry L. Fanburg10Pulmonary Critical Care and Sleep Division, Department of Medicine, Tufts Medical Center, Boston, MA, United StatesFaculty of Medicine of the University of Porto, Cardiovascular Research and Development Center, Porto, PortugalFaculty of Medicine of the University of Porto, Cardiovascular Research and Development Center, Porto, PortugalFaculty of Medicine of the University of Porto, Cardiovascular Research and Development Center, Porto, PortugalPulmonary Critical Care and Sleep Division, Department of Medicine, Tufts Medical Center, Boston, MA, United StatesPulmonary Critical Care and Sleep Division, Department of Medicine, Tufts Medical Center, Boston, MA, United StatesDepartment of Medicine, Atlanta Veterans Affairs and Emory University Medical Centers, Atlanta, GA, United StatesDepartment of Medicine, Emory University, Atlanta, GA, United StatesDepartment of Medicine, Atlanta Veterans Affairs and Emory University Medical Centers, Atlanta, GA, United StatesDepartment of Medicine, Emory University, Atlanta, GA, United StatesPulmonary Critical Care and Sleep Division, Department of Medicine, Tufts Medical Center, Boston, MA, United StatesDiastolic dysfunction of the heart and decreased compliance of the vasculature and lungs (i.e., increased organ tissue stiffness) are known features of obesity and the metabolic syndrome. Similarly, cardiac diastolic dysfunction is associated with aging. Elevation of the enzyme transglutaminase 2 (TG2) leads to protein cross-linking and enhanced collagen synthesis and participates as a candidate pathway for development of tissue stiffness. With these observations in mind we hypothesized that TG2 may be elevated in tissues of a rat model of obesity/metabolic syndrome (the ZSF 1 rat) and a mouse model of aging, i.e., the senescent SAMP8 mouse. In the experiments reported here, TG2 expression and activity were found for the first time to be spontaneously elevated in organs from both the ZSF1 rat and the SAMP8 mouse. These observations are consistent with a hypothesis that a TG2-related pathway may participate in the known tissue stiffness associated with cardiac diastolic dysfunction in these two rodent models. The potential TG2 pathway needs better correlation with physiologic dysfunction and may eventually provide novel therapeutic insights to improve tissue compliance.https://www.frontiersin.org/article/10.3389/fphys.2020.560019/fulltransglutaminase 2heartlungobesitymetabolic syndromeaging |
| spellingShingle | Krishna C. Penumatsa Ines Falcão-Pires Sara Leite Adelino Leite-Moreira Chinmayee D. Bhedi Sabina Nasirova Jing Ma Jing Ma Roy L. Sutliff Roy L. Sutliff Barry L. Fanburg Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging Frontiers in Physiology transglutaminase 2 heart lung obesity metabolic syndrome aging |
| title | Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging |
| title_full | Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging |
| title_fullStr | Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging |
| title_full_unstemmed | Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging |
| title_short | Increased Transglutaminase 2 Expression and Activity in Rodent Models of Obesity/Metabolic Syndrome and Aging |
| title_sort | increased transglutaminase 2 expression and activity in rodent models of obesity metabolic syndrome and aging |
| topic | transglutaminase 2 heart lung obesity metabolic syndrome aging |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.560019/full |
| work_keys_str_mv | AT krishnacpenumatsa increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT inesfalcaopires increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT saraleite increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT adelinoleitemoreira increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT chinmayeedbhedi increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT sabinanasirova increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT jingma increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT jingma increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT roylsutliff increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT roylsutliff increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging AT barrylfanburg increasedtransglutaminase2expressionandactivityinrodentmodelsofobesitymetabolicsyndromeandaging |