Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability

The antioxidant activity of the aqueous extracts from grapefruit seed and peel was evaluated. The seeds and peels were transformed into flour and the flavonoids were extracted by means of organic solvents in the following flour/solvent ratios: 05:25; 1:25 and 2:25. Then, single extractions with 25mL...

Full description

Bibliographic Details
Main Authors: Mario José Moreno Álvarez, Carmen Ysabel Guarán, Douglas Rafael Belén Camacho, David García-Pantaleón, Carlos Alberto Medina Martínez
Format: Article
Language:English
Published: Consejo Superior de Investigaciones Científicas 2007-12-01
Series:Grasas y Aceites
Subjects:
Online Access:http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/446
_version_ 1819298036720861184
author Mario José Moreno Álvarez
Carmen Ysabel Guarán
Douglas Rafael Belén Camacho
David García-Pantaleón
Carlos Alberto Medina Martínez
author_facet Mario José Moreno Álvarez
Carmen Ysabel Guarán
Douglas Rafael Belén Camacho
David García-Pantaleón
Carlos Alberto Medina Martínez
author_sort Mario José Moreno Álvarez
collection DOAJ
description The antioxidant activity of the aqueous extracts from grapefruit seed and peel was evaluated. The seeds and peels were transformed into flour and the flavonoids were extracted by means of organic solvents in the following flour/solvent ratios: 05:25; 1:25 and 2:25. Then, single extractions with 25mL of methanol 85% (v/v) were produced. Afterward, methanolic extracts were mixed in proportion 1:1 (v/v) with petroleum ether and stirred until the appearance of two sharp phases. Subsequently, the aqueous phase was extracted with ethyl acetate and two phases were separated. Doses of every phase in proportion of 0.01%, 0.75% and 0.10%, were added to soybean oil. Samples were stored in an air stove at 60°C ± 1°C and the peroxide value was determined every 24 h for the 600h of study. Significant differences were found between treatments (P< 0.05). An antioxidant effect was shown independently of the metabolite source, flour weight/extraction volume ratio or extract concentration (P < 0.05). The major antioxidant effect showed by the treatments were with 0.01% aqueous extract from 0.5:25 seed flour/solvent ratio, and 0.75% aqueous extract from 0.5:25 peel flour/solvent ratio. No treatment showed pro-oxidant effects. In conclusion, the incorporation of flavonoid extracts into deodorized soybean oil permitted the confirmation of its antioxidant performance.
first_indexed 2024-12-24T05:23:31Z
format Article
id doaj.art-c496b9f10945477193554c22ce10daee
institution Directory Open Access Journal
issn 0017-3495
1988-4214
language English
last_indexed 2024-12-24T05:23:31Z
publishDate 2007-12-01
publisher Consejo Superior de Investigaciones Científicas
record_format Article
series Grasas y Aceites
spelling doaj.art-c496b9f10945477193554c22ce10daee2022-12-21T17:13:23ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42142007-12-0158435135810.3989/gya.2007.v58.i4.446443Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stabilityMario José Moreno Álvarez0Carmen Ysabel Guarán1Douglas Rafael Belén Camacho2David García-Pantaleón3Carlos Alberto Medina Martínez4Universidad Simón Rodríguez, Laboratorio de BiomoléculasUniversidad Simón Rodríguez, Laboratorio de BiomoléculasUniversidad Simón Rodríguez, Laboratorio de BiomoléculasUniversidad Simón Rodríguez, Laboratorio de BiomoléculasUniversidad Simón Rodríguez, Laboratorio de BiomoléculasThe antioxidant activity of the aqueous extracts from grapefruit seed and peel was evaluated. The seeds and peels were transformed into flour and the flavonoids were extracted by means of organic solvents in the following flour/solvent ratios: 05:25; 1:25 and 2:25. Then, single extractions with 25mL of methanol 85% (v/v) were produced. Afterward, methanolic extracts were mixed in proportion 1:1 (v/v) with petroleum ether and stirred until the appearance of two sharp phases. Subsequently, the aqueous phase was extracted with ethyl acetate and two phases were separated. Doses of every phase in proportion of 0.01%, 0.75% and 0.10%, were added to soybean oil. Samples were stored in an air stove at 60°C ± 1°C and the peroxide value was determined every 24 h for the 600h of study. Significant differences were found between treatments (P< 0.05). An antioxidant effect was shown independently of the metabolite source, flour weight/extraction volume ratio or extract concentration (P < 0.05). The major antioxidant effect showed by the treatments were with 0.01% aqueous extract from 0.5:25 seed flour/solvent ratio, and 0.75% aqueous extract from 0.5:25 peel flour/solvent ratio. No treatment showed pro-oxidant effects. In conclusion, the incorporation of flavonoid extracts into deodorized soybean oil permitted the confirmation of its antioxidant performance.http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/446antioxidantsflavonoidsgrapefruitlipidic oxidationoilssoybean
spellingShingle Mario José Moreno Álvarez
Carmen Ysabel Guarán
Douglas Rafael Belén Camacho
David García-Pantaleón
Carlos Alberto Medina Martínez
Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
Grasas y Aceites
antioxidants
flavonoids
grapefruit
lipidic oxidation
oils
soybean
title Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
title_full Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
title_fullStr Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
title_full_unstemmed Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
title_short Effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
title_sort effect of flavonoid extracts from grapefruit seeds and peel flour on soybean oil stability
topic antioxidants
flavonoids
grapefruit
lipidic oxidation
oils
soybean
url http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/446
work_keys_str_mv AT mariojosemorenoalvarez effectofflavonoidextractsfromgrapefruitseedsandpeelflouronsoybeanoilstability
AT carmenysabelguaran effectofflavonoidextractsfromgrapefruitseedsandpeelflouronsoybeanoilstability
AT douglasrafaelbelencamacho effectofflavonoidextractsfromgrapefruitseedsandpeelflouronsoybeanoilstability
AT davidgarciapantaleon effectofflavonoidextractsfromgrapefruitseedsandpeelflouronsoybeanoilstability
AT carlosalbertomedinamartinez effectofflavonoidextractsfromgrapefruitseedsandpeelflouronsoybeanoilstability