Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
Objective: To investigate the effects of Ganjiang granule (GG) on cecal microflora and serum biochemical components in rats with constipation-predominant irritable bowel syndrome (IBS–C). Methods: Twenty-four Sprague–Dawley rats were randomly divided into four groups: control, model, GG, and probiot...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754820300314 |
| _version_ | 1797764340045053952 |
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| author | Lujiao Gao Xin Niu Tingli Niu Xuan Wang Xiaoyan Lu Jiewei Hong Qianjin Feng Xuezhi Yang Roberto González |
| author_facet | Lujiao Gao Xin Niu Tingli Niu Xuan Wang Xiaoyan Lu Jiewei Hong Qianjin Feng Xuezhi Yang Roberto González |
| author_sort | Lujiao Gao |
| collection | DOAJ |
| description | Objective: To investigate the effects of Ganjiang granule (GG) on cecal microflora and serum biochemical components in rats with constipation-predominant irritable bowel syndrome (IBS–C). Methods: Twenty-four Sprague–Dawley rats were randomly divided into four groups: control, model, GG, and probiotics. Rats in the model, GG, and probiotics groups received 3 °C tap water intragastrically; rats in the GG group were treated with GG; rats in the probiotics group were treated with probiotics. For all rats, enzyme-linked immunosorbent assay and colorimetry were used to assess serum biochemical components related to gastrointestinal function; 16S rRNA high-throughput sequencing was used to analyze the cecal microflora. Results: The serum level of 5-hydroxytryptamine (HT) was higher in the model group than in the control group (Z = −2.082, P = .037). The model group exhibited changes in cecal microflora: the relative abundances of Lactobacillus decreased (Z = −2.882, P = .004) and Dorea increased (t = −3.030, P = .023), compared with the control group. The GG and probiotics groups exhibited normal serum levels of 5-HT. The GG and probiotics groups exhibited improved serum levels of gastrin; the probiotics group exhibited an improved serum level of vasoactive intestinal peptide. Compared with the model group, The GG group exhibited greater relative abundance of Ruminococcus (Z = −2.402, P = .016); the probiotics group exhibited greater relative abundance of SMB53 (Z = −2.823, P = .005) and lower relative abundances of Desulfovibrio (Z = −2.823, P = .005) and Facklamia (Z = −2.608, P = .009). Conclusion: The effects of GG on IBS-C may be related to regulation of the serum level of 5-HT, as well as elevated relative abundance of beneficial bacteria in cecal microflora. |
| first_indexed | 2024-03-12T19:54:18Z |
| format | Article |
| id | doaj.art-c49a349bb98e4dbeb3dab1bc0ad514ac |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T19:54:18Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-c49a349bb98e4dbeb3dab1bc0ad514ac2023-08-02T02:55:07ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482020-04-0172148153Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndromeLujiao Gao0Xin Niu1Tingli Niu2Xuan Wang3Xiaoyan Lu4Jiewei Hong5Qianjin Feng6Xuezhi Yang7Roberto González8School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China; Corresponding author.Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, 100078, ChinaGraduate School, Shanxi University of Chinese Medicine, Taiyuan, 030619, ChinaBeijing University of Chinese Medicine Libraries, Beijing, 100029, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, ChinaShanxi Modern Chinese Medicine Engineering Laboratory, Shanxi University of Chinese Medicine, Taiyuan, 030619, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, ChinaNational Cancerology Institute of Mexico, National Polytechnic University of Mexico, Mexico City, 07738, MexicoObjective: To investigate the effects of Ganjiang granule (GG) on cecal microflora and serum biochemical components in rats with constipation-predominant irritable bowel syndrome (IBS–C). Methods: Twenty-four Sprague–Dawley rats were randomly divided into four groups: control, model, GG, and probiotics. Rats in the model, GG, and probiotics groups received 3 °C tap water intragastrically; rats in the GG group were treated with GG; rats in the probiotics group were treated with probiotics. For all rats, enzyme-linked immunosorbent assay and colorimetry were used to assess serum biochemical components related to gastrointestinal function; 16S rRNA high-throughput sequencing was used to analyze the cecal microflora. Results: The serum level of 5-hydroxytryptamine (HT) was higher in the model group than in the control group (Z = −2.082, P = .037). The model group exhibited changes in cecal microflora: the relative abundances of Lactobacillus decreased (Z = −2.882, P = .004) and Dorea increased (t = −3.030, P = .023), compared with the control group. The GG and probiotics groups exhibited normal serum levels of 5-HT. The GG and probiotics groups exhibited improved serum levels of gastrin; the probiotics group exhibited an improved serum level of vasoactive intestinal peptide. Compared with the model group, The GG group exhibited greater relative abundance of Ruminococcus (Z = −2.402, P = .016); the probiotics group exhibited greater relative abundance of SMB53 (Z = −2.823, P = .005) and lower relative abundances of Desulfovibrio (Z = −2.823, P = .005) and Facklamia (Z = −2.608, P = .009). Conclusion: The effects of GG on IBS-C may be related to regulation of the serum level of 5-HT, as well as elevated relative abundance of beneficial bacteria in cecal microflora.http://www.sciencedirect.com/science/article/pii/S2095754820300314Ganjiang granuleProbioticsConstipation-predominant irritable bowel syndromeCecal microflora16S rRNA high-throughput sequencing |
| spellingShingle | Lujiao Gao Xin Niu Tingli Niu Xuan Wang Xiaoyan Lu Jiewei Hong Qianjin Feng Xuezhi Yang Roberto González Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome Journal of Traditional Chinese Medical Sciences Ganjiang granule Probiotics Constipation-predominant irritable bowel syndrome Cecal microflora 16S rRNA high-throughput sequencing |
| title | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
| title_full | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
| title_fullStr | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
| title_full_unstemmed | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
| title_short | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
| title_sort | ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation predominant irritable bowel syndrome |
| topic | Ganjiang granule Probiotics Constipation-predominant irritable bowel syndrome Cecal microflora 16S rRNA high-throughput sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2095754820300314 |
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