Generation of human female reproductive tract epithelium from human embryonic stem cells.
BACKGROUND: Recent studies have identified stem/progenitor cells in human and mouse uterine epithelium, which are postulated to be responsible for tissue regeneration and proliferative disorders of human endometrium. These progenitor cells are thought to be derived from Müllerian duct (MD), the prim...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3115988?pdf=render |
| _version_ | 1818597408938917888 |
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| author | Louie Ye Robyn Mayberry Camden Y Lo Kara L Britt Edouard G Stanley Andrew G Elefanty Caroline E Gargett |
| author_facet | Louie Ye Robyn Mayberry Camden Y Lo Kara L Britt Edouard G Stanley Andrew G Elefanty Caroline E Gargett |
| author_sort | Louie Ye |
| collection | DOAJ |
| description | BACKGROUND: Recent studies have identified stem/progenitor cells in human and mouse uterine epithelium, which are postulated to be responsible for tissue regeneration and proliferative disorders of human endometrium. These progenitor cells are thought to be derived from Müllerian duct (MD), the primordial female reproductive tract (FRT). METHODOLOGY/PRINCIPAL FINDINGS: We have developed a model of human reproductive tract development in which inductive neonatal mouse uterine mesenchyme (nMUM) is recombined with green fluorescent protein (GFP)-tagged human embryonic stem cells (hESCs); GFP-hESC (ENVY). We demonstrate for the first time that hESCs can be differentiated into cells with a human FRT epithelial cell phenotype. hESC derived FRT epithelial cells emerged from cultures containing MIXL1(+) mesendodermal precursors, paralleling events occurring during normal organogenesis. Following transplantation, nMUM treated embryoid bodies (EBs) generated epithelial structures with a typical MD phenotype that expressed the MD markers PAX2, HOXA10. Functionally, the hESCs derived FRT epithelium responded to exogenous estrogen by proliferating and secreting uterine-specific glycodelin A (GdA). CONCLUSIONS/SIGNIFICANCE: These data show nMUM can induce differentiation of hESC to form the FRT epithelium. This may provide a model to study early developmental events of the human FRT. |
| first_indexed | 2024-12-16T11:47:20Z |
| format | Article |
| id | doaj.art-c49d54c8c7014be3a2210e55d76248e3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T11:47:20Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c49d54c8c7014be3a2210e55d76248e32022-12-21T22:32:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2113610.1371/journal.pone.0021136Generation of human female reproductive tract epithelium from human embryonic stem cells.Louie YeRobyn MayberryCamden Y LoKara L BrittEdouard G StanleyAndrew G ElefantyCaroline E GargettBACKGROUND: Recent studies have identified stem/progenitor cells in human and mouse uterine epithelium, which are postulated to be responsible for tissue regeneration and proliferative disorders of human endometrium. These progenitor cells are thought to be derived from Müllerian duct (MD), the primordial female reproductive tract (FRT). METHODOLOGY/PRINCIPAL FINDINGS: We have developed a model of human reproductive tract development in which inductive neonatal mouse uterine mesenchyme (nMUM) is recombined with green fluorescent protein (GFP)-tagged human embryonic stem cells (hESCs); GFP-hESC (ENVY). We demonstrate for the first time that hESCs can be differentiated into cells with a human FRT epithelial cell phenotype. hESC derived FRT epithelial cells emerged from cultures containing MIXL1(+) mesendodermal precursors, paralleling events occurring during normal organogenesis. Following transplantation, nMUM treated embryoid bodies (EBs) generated epithelial structures with a typical MD phenotype that expressed the MD markers PAX2, HOXA10. Functionally, the hESCs derived FRT epithelium responded to exogenous estrogen by proliferating and secreting uterine-specific glycodelin A (GdA). CONCLUSIONS/SIGNIFICANCE: These data show nMUM can induce differentiation of hESC to form the FRT epithelium. This may provide a model to study early developmental events of the human FRT.http://europepmc.org/articles/PMC3115988?pdf=render |
| spellingShingle | Louie Ye Robyn Mayberry Camden Y Lo Kara L Britt Edouard G Stanley Andrew G Elefanty Caroline E Gargett Generation of human female reproductive tract epithelium from human embryonic stem cells. PLoS ONE |
| title | Generation of human female reproductive tract epithelium from human embryonic stem cells. |
| title_full | Generation of human female reproductive tract epithelium from human embryonic stem cells. |
| title_fullStr | Generation of human female reproductive tract epithelium from human embryonic stem cells. |
| title_full_unstemmed | Generation of human female reproductive tract epithelium from human embryonic stem cells. |
| title_short | Generation of human female reproductive tract epithelium from human embryonic stem cells. |
| title_sort | generation of human female reproductive tract epithelium from human embryonic stem cells |
| url | http://europepmc.org/articles/PMC3115988?pdf=render |
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